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1.  Rapid generation of random mutant libraries 
Bioengineered Bugs  2010;1(5):337-340.
A simple and efficient method utilizing in vivo recombination to create recombinant libraries incorporating the products of PCR amplification is described. This will be especially useful for generating large pools of randomly mutagenized clones after error-prone PCR mutagenesis. Here we investigate various parameters to optimize this approach and we demonstrate that as little as 1 pmole of PCR fragment can generate a library with greater than 104 clones in a single transformation without ligation.
doi:10.4161/bbug.1.5.12942
PMCID: PMC3037583  PMID: 21326833
in vivo cloning; lambda red proteins; error prone PCR; mutant libraries; directed evolution

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