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Akgün, Asiye (1)
Chung, Hae Young (1)
Devaney, Jan (1)
Erden Inal, Mine (1)
Finckh, Barbara (1)
Hong, Seong Eui (1)
Jacobson, Arnold (1)
Johnson, W. Thomas (1)
Kahraman, Ahmet (1)
Kim, Chul Hong (1)
Kohlschütter, Alfried (1)
Levy, Wayne C. (1)
Madden, Kenneth M. (1)
Newman, Samuel M. (1)
Park, Dae Ui (1)
Philips, Neena (1)
Stangneth, Birgit (1)
Stratton, John R. (1)
Stuerenburg, Hans-Joerg (1)
Yu, Byung Pal (1)
Year of Publication
The effects of exogenous glutathione on reduced glutathione level, glutathione peroxidase and glutathione reductase activities of rats with different ages and gender after whole-body Γ-irradiation
Erden Inal, Mine
Age-and gender-related changes on reduced glutathione (GSH) level, glutathione peroxidase (GPx) and glutathione reductase (GR) activities in the liver of rat exposed to different dose of whole-body g-ray irradiation were determined. In addition, the effect of administration of exogenous GSH on endogenous GSH levels, GPx and GR activities was investigated. For this aim, male and female rats aged 1 and 5 moths were divided into two groups as g-ray and g-ray+GSH. Both groups were again divided into four groups as irradiated with 2, 4, 6 and 8 Gy doses. GSH level and GPx activity did not change with age while GR activity was decreased with age. Gender-dependent changes in GPx and GR activities were observed, but GSH values were not affect by sex. GSH levels, GPx and GR activities were not observed dose-associated changes of g-irradiation. GSH level and GPx activity in the 8Gy group were increased by GSH. GR activities of old male rats were found to be increased by glutathione in the 6 and 8Gy groups. These results indicate that radiation and administration of exogenous GSH affect gender-and age-dependent GSH level, GPx and GR activities in the rats.
Beneficial regulation of type I collagen and matrixmetalloproteinase-1 expression by estrogen, progesterone, and its combination in skin fibroblasts.
There is impaired wound healing and loss of type I collagen in skin aging, which can be improved by topical estrogen in vivo. The goal of this study was to determine the effects of estrogen, and progesterone and a combination of estrogen and progesterone as well, on the proliferation and the expression of type I collagen and matrixmetalloprotienase-1 (MMP-1, degrades collagen) in dermal fibroblasts (cells that synthesize collagen and MMP-1) in-vitro. Estrogen, progesterone, and its combination similarly and significantly inhibited cell proliferation and MMP-1 protein levels, and simultaneously stimulated type I collagen expression in the fibroblasts, indicating beneficial modulation.
Copper deficiency: A potential model for determining the role of mitochondria in cardiac aging
Johnson, W. Thomas
Newman, Samuel M.
Heart mitochondria experience age-related declines in cytochrome c oxidase (CCO) activity and increases in the generation of reactive oxygen species (ROS) that may contribute to loss of cardiac function and the development of disease that occur with advancing age. In a manner similar to aging, copper deficiency also suppresses heart CCO activity and has cardiovascular consequences related to increased peroxidation. Food restriction is often used as a tool to study oxidative mechanisms of aging and the present study examines the potential of copper deficiency to model the role of mitochondria in cardiac aging by determining if the effect of food restriction on CCO activity and oxidative stress in heart mitochondria parallels its effect on cardiac mitochondria during aging. Overall, copper deficiency severely inhibited CCO activity and increased both Mn superoxide dismutase (MnSOD) and glutathione peroxidase (GPX) in isolated heart mitochondria. However, a 20% reduction in food intake by copper-deficient rats increased CCO activity by 65% and decreased MnSOD activity by 25% but had no effect in rats fed adequate copper. Copper deficiency also reduced the carbonyl content of 80–100 kDa mitochondrial proteins, but the reduction in carbonyl content was unaffected by food restriction. Food restriction did, however, completely prevent the enlargement of cardiac mitochondria in copper-deficient rats. Together, these findings indicate that copper deficiency induces mitochondrial antioxidant enzyme activity and hypertrophy in cardiac tissue in response to reduced CCO activity and that food restriction may counteract these changes by reducing oxidative stress. Because the action of food restriction on CCO activity and mitochondrially generated oxidative stress are similar in copper deficiency and aging, copper deficiency may serve as a short-term model for studying the potential roles of mitochondria in cardiac aging.
AgingDB: A database for oxidative stress and calorie restriction in the study of aging
Park, Dae Ui
Kim, Chul Hong
Hong, Seong Eui
Yu, Byung Pal
Chung, Hae Young
Aging can be characterized in all living organisms as the inevitable biological changes that occur with advancing age. The aging process is time-dependent and leads to functional declines and increased incidences of disease. The underlying pathphysiologic processes of aging may best be explained using several interacting biological processes: genomic activity, oxidative stress, and age-related disease processes, all of which modify the rate and progression of aging. In this report, we describe a database, termed AgingDB, used to retrieve information on the biomolecules known to be modulated during the aging process and by the life-prolonging action of caloric restriction (CR). To enhance the usefulness of AgingDB, we include data collected from studies of CR’s anti-oxidative action on gene expression, oxidative stress, and many chronic age-related diseases. We organized AgingDB into two sections A) apoptosis and the various mitochondrial biomolecules that play a role in aging; B) nuclear transcription factors known to be_sensitive to oxidative environment. AgingDB features an imagemap of biomolecular signal pathways and visualized information that includes protein-protein interactions of biomolecules. Authorized users can submit a new biomolecule or edit an existing biomolecule to reflect latest developments. By making available the most update information through AgingDB, we expect to assist researchers who are exploring the molecular basis of age-related changes modified by the life-prolonging action of CR. For the reader’s convenience and accessibility, AgingDB is freely available at http://agingdb.bio.pusan.ac.kr/.
The effect of aging on phenylephrine response in normal subjects
Madden, Kenneth M.
Levy, Wayne C.
Stratton, John R.
INTRODUCTION: With aging, cardiac responses to β-adrenergic stimulation decline but the responses to α1-stimulation are less clear. Moreover, whether aging, in the absence of disease, influences the left ventricular response to an increase in afterload is unclear. This study examined the effect of aging on heart rate (HR), blood pressure (BP), cardiac index (CI) and several left ventricular contractility measurements during α 1-stimulation with a phenylephrine infusion. METHODS: Subjects were rigorously screened to be normal by history, physical, blood tests, ECG, ETT and echocardiogram. Twelve young (mean 26 years, all male) and 15 aged (69 years, 11 males) subjects were studied during 10 minute infusions of phenylephrine at 0.5 and 1.0 mcg/ kg/min. HR, BP and radionuclide ventriculographic cardiac volumes were measured. RESULTS: Systolic BP increased more in the aged than in the young (22 vs. 13%, p=0.003), while heart rate (16 vs. 21%, p=0.05) fell less. Contractile responses to phenylephrine, including EF, stroke volume index (SVI), stroke work index and left ventricular contractility index were not altered with aging. Systemic vascular resistance (SVR) was higher at baseline and at each infusion rate, but there was no age-associate change in the response to PE. CONCLUSIONS: In a healthy normal aged population, a preserved SVI response in the setting of a higher baseline SVR results in an increased SBP response to α1-stimulation. Contractile responses to increased afterload are not altered with aging. Age-associated differences in the response to α1-stimulation are small and are explained by altered baroreflex sensitivity and a stiffer vasculature.
Influence of diagnostic categories, age, and gender on antioxidative defense and lipid peroxidation in skeletal muscle of patients with neuromuscular diseases
The influence of diagnostic categories, age, and gender on parameters of oxidative stress measured in 102 patients with neuromuscular diseases and 11 control subjects was assessed using a stepwise multiple linear regression model. Antioxidative enzyme activities, lipophilic antioxidants, and lipid peroxidation were analyzed in muscle biopsies.
Mitochondrial myopathies and amyotrophic lateral sclerosis (ALS) are thought to be particularly susceptible to increased oxidative stress. In our study, mitochondrial myopathies emerged as a positive predictor of malondialdehyde (p < 0.05) and ALS as a negative predictor of alpha-tocopherol (p < 0.05). Although the primary atrophic process in ALS is not in muscle but in motoneurons, this finding could have therapeutic implications, as such patients might benefit from antioxidant supplementation.
In our study age emerged as a negative predictor of the coenzyme Q10 concentration (p < 0.003), whereas the percentage of reduced coenzyme Q10 remained unchanged. Age emerged as a positive predictor of the activities of catalase (p < 0.01) and superoxide dismutase (p < 0.002), probably reflecting an enzymatic upregulation that compensates for the loss of coenzyme Q10. The increased activities of catalase and superoxide dismutase in females compared to males indicate a higher antioxidative potential in female muscle. Whether this increase contributes to a higher life expectancy of women remains to be investigated.
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