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jtitle_s:("Age (dorer)")
1.  Resveratrol, but not dihydroresveratrol, induces premature senescence in primary human fibroblasts 
Age  2011;33(4):555-564.
Resveratrol, trans-3,5,4′-trihydroxystilbene, is a polyphenolic compound which has been reported to mimic the gene expression patterns seen in whole animals undergoing dietary restriction. The mechanism of action of resveratrol remains poorly understood, but modulation of both cellular proliferation and apoptosis has been proposed as important routes by which the molecule may exert its effects. This study reports the effects of both resveratrol and dihydroresveratrol (a primary in vivo metabolite) on the proliferative capacity of human primary fibroblasts. No generalised reduction in the growth fraction was observed when fibroblasts derived from three different tissues were treated with resveratrol at concentrations of 10 μm or less. However, concentrations above 25 μm produced a dose-dependent reduction in proliferation. This loss of the growth fraction was paralleled by an increase in the senescent fraction as determined by staining for senescence associated beta galactosidase and dose recovery studies conducted over a 7-day period. Entry into senescence in response to treatment with resveratrol could be blocked by a 30-min preincubation with the p38 MAP kinase inhibitor SB203580. No effects on proliferation were observed when cells were treated with dihydroresveratrol at concentrations of up to 100 μm.
doi:10.1007/s11357-010-9201-5
PMCID: PMC3220406  PMID: 21318333
Resveratrol; Cellular senescence; Ageing; MRC5; HCA2; Ek1.Br; p38 MAP kinase
2.  Back to the future? Transatlantic collaboration on ageing research 
Age  2010;31(4):257-259.
doi:10.1007/s11357-009-9127-y
PMCID: PMC2813044  PMID: 21119825
3.  Chemical changes in aging Drosophila melanogaster 
Age  2009;31(4):343-351.
The “Green Theory” of aging proposes that organismal lifespan is limited by the failure to repair molecular damage generated by a broad range of metabolic processes. Two specific predictions arise from this: (1) that these processes will produce a wide variety of stable but dysfunctional compounds that increase in concentration with age, and (2) that organisms maintained under conditions that extend lifespan will display a reduced rate of accumulation of such “molecular rubbish”. To test these predictions, novel analytical techniques were developed to investigate the accumulation of damaged compounds in Drosophila melanogaster. Simple preparative techniques were developed to produce digests of whole D. melanogaster for use in three-dimensional (3D) fluorimetry and 1H NMR spectrometry. Cohorts of Drosophila maintained under normal conditions showed an age-related increase in signals consistent with damage whereas those maintained under conditions of low temperature and dietary restriction did not. 1H NMR revealed distinct age-associated spectral changes that will facilitate the identification of novel compounds that both increase and decrease during aging in this species. These findings are consistent with the predictions of the “Green Theory”.
doi:10.1007/s11357-009-9105-4
PMCID: PMC2813051  PMID: 19585275
Drosophila melanogaster; Aging; Green theory; Oxidative damage; Glycation; Spectroscopy

Results 1-3 (3)