Search tips
Search criteria

Results 1-1 (1)

Clipboard (0)
Year of Publication
Document Types
jtitle_s:("Age (dodr)")
1.  Effects of oxygen, growth state, and senescence on the antioxidant responses of WI-38 fibroblasts 
Age  2010;32(4):435-449.
Mitotically active, growth-arrested cells and proliferatively senescent cultures of human fetal lung fibroblasts (WI-38) were exposed to six different oxygen tensions for various lengths of time and then analyzed to determine the responses of their antioxidant defense system. Glutathione (GSH) concentration increased as a function of ambient oxygen tension in early passage cultures; the effect was larger in exponentially growing cultures than in those in a state of contact-inhibited growth arrest, but was absent in senescent cells. Conversely, the activity of glutathione disulfide reductase was greater in growth-arrested cultures than in mitotically active cells irrespective of oxygen tension. Glucose-6-phosphate dehydrogenase was lowest in log-phase cells exposed to different oxygen tensions for 24 h and in senescent cells. Both hypoxia and hyperoxia depressed selenium-dependent glutathione peroxidase activity in early passage cultures, while the activity of the enzyme progressively declined with increasing oxygen in senescent cells. The GSH S-transferase activity was unresponsive to changes in ambient oxygen tension in either young or senescent cultures. Manganese-containing superoxide dismutase (MnSOD) activity was unaffected by oxygen tension, but was elevated in young confluent cultures as compared with cultures in log-phase growth. MnSOD activity was significantly higher in senescent cultures than in early passage cultures and was also responsive to increased oxygen tension in senescent cultures. Copper–zinc-containing superoxide dismutases activity was not affected by oxygen tension or the passage of time, but it declined in senescent cultures.
PMCID: PMC2980593  PMID: 20473639
Oxygen; Antioxidant defenses; Senescence; WI-38; Fibroblasts; Glucose-6-phosphate dehydrogenase; Superoxide dismutase; Glutathione; GSSG reductase; Glutathione peroxidase; Reactive oxygen species

Results 1-1 (1)