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1.  Study on Optimisation of Extraction Process of Tanshinone IIA and Its Mechanism of Induction of Gastric Cancer SGC7901 Cell Apoptosis 
The objective of this paper was to investigate the extraction process of tanshinone IIA and its mechanism of induction of gastric cancer SGC7901 cell apoptosis. Extraction process of tanshinone IIA was optimised by orthogonal experimental method, and its effect on gastric cancer SGC7901 cell apoptosis was observed using MTT assay and electron microscopy. The optimum extraction process of tanshinone IIA was as follows: addition of a 10-fold amount of 80% ethanol, one-time extraction, and extraction time of 45 minutes. The study concluded that tanshinone IIA can induce apoptosis of gastric cancer SGC7901 cells.
PMCID: PMC3847383  PMID: 24311868
Tanshinone IIA; MTT assay; gastric cancer SGC7901 cells
2.  A Study on the Protective Effect of Silybum Marianum Extract on Hepatic Ischemia-Reperfusion Injury 
The objective of the study was to study the protective effect of Silybum marianum extract on hepatic ischemia-reperfusion injury. Rats were randomly divided into five groups; namely Silybum marianum extract high-, medium-, and low-dose protection groups, model group and control group. Hepatic ischemia-reperfusion injury model was prepared. Serum or plasma AST, ALT, MDA, TNF-α, IL-1β, IL-6 levels were measured. The results revealed that after liver injury, AST, ALT, MDA, TNF-α, IL-1β, and IL-6 levels significantly increased in succession, showing significant differences. We concluded that inflammatory cytokines participate in liver injury and that Silybum marianum extract can reduce the production of inflammatory cytokines, and thus can have a protective effect on hepatic ischemia and reperfusion.
PMCID: PMC3847421  PMID: 24311841
Silybum marianum; hepatic ischemia-reperfusion injury; protective effect
3.  The Efficacy of Crude Extract of Aloe Secundiflora on Candida Albicans 
In-vitro studies on the efficacy of crude extracts of Aloe secundiflora on Candida albicans was conducted. Five mature leaves of Aloe secundiflora were collected and the crude extract was prepared, then autoclaved. The extract was then tested on Candida albicans grown on solid media. The results from these studies revealed complete inhibition of Candida albicans growth caused by Aloe secundiflora crude extract on solid media. The findings from this study suggest that the crude extract of Aloe secundiflora can inhibit the growth of Candida albicans. Further studies are required to establish the in-vivo activity of the crude extract, the active ingredient, dosage and safety of Aloe secundiflora, before recommending for clinical use.
PMCID: PMC2816467  PMID: 20606782
Aloe secundiflora; alternative medicine; opportunistic infections; crude extract
4.  Cytotoxic Activity of Selected Nigerian Plants 
Cancer is one of the most prominent human diseases which has stimulated scientific and commercial interest in the discovery of new anticancer agents from natural sources. The current study investigates the cytotoxic activity of ethanolic extracts of sixteen Nigerian plants used locally for the treatment of cancer using the MTT assay on the HeLa cell line. Sapium ellipticum leaves showed activity comparable to the reference compound Cisplatin and greater cytotoxic activity than Combretum paniculatum, Celosia trigyna, Drymaria cordata, Cyathula achyranthoides and Cyathula prostata. Justica extensa, Pupalia lappacea, Hedranthera barteri leaves, Alternanthera sessilis, Ethulia conyzoides leaves, Combretum zenkeri root, Sapium ellipticum stembark and Lannea nigritana stembark showed very low activity while Combretum molle, Adenanthera parvoniana and Lannea acida showed no activity. The results justify the use of Sapium, Combretum, Celosia, Drymaria and Cyathula in traditional treatment of cancer.
PMCID: PMC2816471  PMID: 20606772
Medicinal plants; Cytotoxicity; Cancer; Sapium; Combretum
5.  Antidiabetic Activity of Gladiolus Psittascinus in Alloxan Induced Diabetic Rats 
The methanol extract of Gladiolus psittascinus bulb was evaluated for its antidiabetic activities in alloxan-induced diabetic rats. Blood glucose levels of the glucose loaded and alloxan-induced diabetic rats were estimated over 180 minutes using the O-toluidine and glucose-oxidase methods. The methanol extract at 1g/kg dose exhibited 16.2% decrease in blood glucose level in the glucose loaded rats and a peak effect of 78.9% in the alloxan-induced diabetic rats. The extract exhibited significant blood glucose lowering effects in the oral glucose tolerance test and type 2 diabetic rats. This study shows a possible beneficial effect of Gladiolus psittascinus in the management of non-insulin dependent diabetes (NIDDM).
PMCID: PMC2816533  PMID: 20161931
Gladiolus psittascinus; hypoglycaemic activity; diabetes mellitus
6.  Screening of Traditionally Used Endemic Soqotraen Plants for Cytotoxic Activity 
Thirty extracts obtained from 10 endemic plant species belonging to 8 plant families used in the traditional medicine in Socotra have been tested for cytotoxic activity against FL-cells. Extracts of Eureiandra balfourii and Commiphora ornifolia showed the strongest activity against FL-cells with IC50 < 10 µg/ml and 39.3 µg/ml respectively.
PMCID: PMC2816517  PMID: 20161922
Cytotoxicity; Soqotra; Fl-cells
7.  Naphthaquinones of Alkanna Orientalis (L.) BOISS 
The roots of Alkanna orientalis (L.) Boiss yielded α- methyl-n-butyl alkannin (compound 1) and alkannin acetate (compound 2). The compounds were identified by UV, MS, 1H NMR and 13C NMR. Quantitative determination of α- methyl-n-butyl alkannin and alkannin acetate in Alkanna orientalis (L) Boiss roots was established by TLC densitometry.
PMCID: PMC2816436  PMID: 20162072
Alkanna orientalis; naphthaquinones; NMR; α- methyl-n-butyl alkannin; alkannin acetate; TLC densitometry

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