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issn:1567-133
1.  Expression of cadherin10, a type II classic cadherin gene, in the nervous system of the embryonic zebrafish 
Gene expression patterns : GEP  2006;6(7):703-710.
Cadherins are cell surface adhesion molecules that play important roles in development of tissues and organs. In this study we analyzed expression pattern of cadherin10, a member of the type II classic cadherin subfamily, in the embryonic zebrafish using in situ hybridization methods. cadherin10 message (cdh10) is first and transiently expressed by the notochord. In the developing nervous system, cdh10 was first detected in a subset of the cranial ganglia, then in restricted brain regions and neural retina. As development proceeds, cdh10 expression domain and/or expression levels increased in the embryonic nervous system. Our results show that cdh10 expression in the zebrafish developing nervous system is both spatially and temporally regulated.
doi:10.1016/j.modgep.2005.12.009
PMCID: PMC2562320  PMID: 16488669
zebrafish; development; cell adhesion molecules; brain; cranial and lateral line ganglia
2.  Cloning and expression analysis of cadherin7 in the central nervous system of the embryonic zebrafish 
Gene expression patterns : GEP  2006;7(1-2):15-22.
Cadherin cell adhesion molecules exhibit unique expression patterns during development of the vertebrate central nervous system. In this study we obtained a full-length cDNA of a novel zebrafish cadherin using reverse transcriptase-polymerase chain reaction (RT-PCR) and 5′ and 3′ rapid amplification of cDNA ends (RACE). The deduced amino acid sequence of this molecule is most similar to the published amino acid sequences of chicken and mammalian cadherin7 (Cdh7), a member of the type II cadherin subfamily. cadherin7 message (cdh7) expression in embryonic zebrafish was studied using in situ hybridization and RT-PCR methods. cdh7 expression begins at about 12 hours post fertilization (hpf) in a small patch in the anterior neural keel, and along the midline of the posterior neural keel. By 24 hpf, cdh7 expression in the brain shows a distinct segmental pattern that reflects the neuromeric organization of the brain, while its expression domain in the spinal cord is continuous, but confined to the middle region of the spinal cord. As development proceeds, cdh7 expression is detected in more regions of the brain, including the major visual structures in the fore- and midbrains, while its expression domain in the hindbrain becomes more restricted, and its expression in the spinal cord becomes undetectable. cdh7 expression becomes reduced in 3-day old embryos. Our results show that cdh7 expression in the zebrafish developing central nervous system is both spatially and temporally regulated.
doi:10.1016/j.modgep.2006.05.002
PMCID: PMC1716651  PMID: 16774849
zebrafish; development; cell adhesion molecules; brain; spinal cord; visual system

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