Objective: To evaluate amniotic fluid glucose, matrix metalloproteinase (MMP)-9, interleukin (IL)-6, and IL-12 for
diagnosing subclinical chorioamnionitis in women with preterm labor.
Methods: Forty-four women in preterm labor at 22–35 weeks gestation with suspected subclinical
chorioamnionitis underwentamniocentesis.Amniotic fluid analysis included Gram stain, culture, and determination
of glucose, MMP-9, IL-6, and IL-12 concentrations. Median values of these analytes were compared using the
Mann-Whitney U test. Sensitivity, specificity, and positive and negative predictive values were calculated for tests
using a positive amniotic fluid culture or delivery within 24 hours as the key outcome variables
Results: Amniotic fluid concentrations of glucose, MMP-9, and IL-6 correlated closely with positive culture or
delivery within 24 hours. IL-12 concentrations did not correlate with either a positive culture or delivery within
Conclusions: Amniotic fluid glucose, MMP-9, and IL-6 reliably predict microbial invasion of the amniotic cavity or
imminent delivery. IL-12 values did not correlate with amniotic fluid culture results or imminent delivery.