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Archives of Disease in Childhood (1)
BMJ : British Medical Journal (1)
Journal of Clinical Pathology (1)
Molecular and Cellular Biology (1)
Hodgkin, J (1)
Hornsey, V S (1)
Kondo, K (1)
Morley, David (1)
Parkin, M (1)
Prowse, C V (1)
Waterston, A (1)
Waterston, R H (1)
Waterston, Tony (1)
Waterston, Y G (1)
Year of Publication
Battle of the bottle
BMJ : British Medical Journal
Inequalities in child health.
Archives of Disease in Childhood
Differential expression of five tRNA(UAGTrp) amber suppressors in Caenorhabditis elegans.
, R H
Molecular and Cellular Biology
Caenorhabditis elegans has 12 tRNA(UGGTrp) genes as defined by Southern analysis. In order to evaluate the function of the individual members of this multigene family, we sought to recover amber (UAG)-suppressing mutations from reversion experiments with animals carrying amber mutations in a nervous system-affecting gene (unc-13) or a sex-determining gene (tra-3). Revertants were analyzed by Southern blot, exploiting the fact that the CCA to CTA change at the anticodon creates a new XbaI site. Five different members of the tRNATrp gene family were identified as suppressors: sup-7 X, sup-5 III, sup-24 IV, sup-28 X, and sup-29 IV. All five suppressor genes were sequenced and found to encode identical tRNA(UAGTrp) molecules with a single base change (CCA to CTA) at the anticodon compared with their wild-type counterparts. The flanking sequences had only limited homology. The relative expression of these five genes was determined by measuring the efficiencies of suppressers against amber mutations in genes affecting the nervous system, hypodermis, muscle, and sex determination. The results of these cross-suppression tests showed that the five members of the tRNA(Trp) gene family were differentially regulated in a tissue- or development stage-specific manner.
Artificial factor VIII deficient plasma: preparation using monoclonal antibodies and its use in one stage coagulation assays.
Hornsey, V S
, Y G
Prowse, C V
Journal of Clinical Pathology
Monoclonal antibodies to factor VIII antigen (VIII:Ag) and von Willebrand factor (vWf:Ag) were immobilised on Sephacryl S-1000 and tested for their ability to deplete normal human citrated plasma of factor VIII. A combination of two antibodies to VIII:Ag and one antibody to vWf:Ag was required to produce plasma containing less than 0.01 IU/ml. Its performance in the one stage coagulation assay of VIII:C was equivalent to that of congenital VIII deficient plasma for the assay of normal and haemophilic plasma and factor VIII concentrates. Storage of freeze dried aliquots of this product at -20 degrees C, +4 degrees C, and 37 degrees C showed that it could be used as a substrate for at least six months when stored at temperatures +4 degrees C and below.
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