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1.  Use of a Novel Method to Find Substrates of Protein Kinase C Delta Identifies M2 Pyruvate Kinase 
Protein kinase C (PKC) family members have been implicated in numerous cellular processes. However, identifying the substrates of each PKC isozyme remains a challenge. Here, we describe a method using two dimensional (2-D) isoelectric focusing gel electrophoresis to identify substrates of delta PKC (δPKC) in MCF-7 breast carcinoma cells. We show that M2 pyruvate kinase is a substrate of δPKC, and further characterize the interaction between M2 pyruvate kinase and δPKC in MCF-7 cells by immunoprecipitation. δPKC activation in vitro or in cells did not appear to alter the enzyme activity or polymerization of M2 pyruvate kinase.
PMCID: PMC1931518  PMID: 17337233
Protein Kinase C; Signal transduction; M2-type pyruvate kinase; Heat shock protein 27

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