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Applied Microbiology (1)
Canadian Journal of Comparative Medicine (1)
Canadian Medical Association Journal (1)
Barnick, J. A. (1)
Boyd, E. M. (1)
Brashear, D. A. (1)
Carey, P. F. (1)
Liu, C. K. (1)
Liu, O. C. (1)
Liu, S. J. (1)
Ryu, E. (1)
Seraichekas, H. R. (1)
Year of Publication
Viral Depuration by Assaying Individual Shellfish1
Seraichekas, H. R.
Brashear, D. A.
Barnick, J. A.
Carey, P. F.
, O. C.
A study was carried out to further evaluate the practicability of viral depuration by assaying individual shellfish. The Northern quahaug and a strain of the type 1 attenuated poliovirus were used as the working model. Two types of depuration systems were employed: the small experimental tanks and a pilot-size tank with a capacity of approximately 24 bushels (836 liters) of shellfish. Volumes of the individual shellfish samples were found uniform throughout the experiments when a prior selection for the weight of the shellfish was made. There was also no significant difference in volumes of the individual samples during the course of depuration (24 to 96 hr). Under controlled hydrographic conditions, however, the uptake of virus in individual shellfish varied considerably. In general, the individual variability reached 10- to 100-fold. This wide variation would explain the variability of viral contents obtained in pooled samples during depuration as reported previously. During a later phase of depuration, although a great majority of shellfish were free of the virus, a few still harbored minimal amounts of contaminants. The presence of virus in some of the shellfish after various periods of depuration would, theoretically, be obscured by the pooling of the sampled shellfish. Further examination of the negative samples by assaying larger quantities than those routinely used revealed that a few still contained virus. To simulate naturally polluted shellfish as closely as technically possible, shellfish were polluted with minimal amounts of virus. The shellfish were cleansed more rapidly by the depuration process than were those polluted with more virus. Since the naturally polluted shellfish were shown to contain less virus than those studied in the laboratory, it is anticipated that the former type of shellfish may be cleansed more readily by this process within a reasonable period of time. Justification for a field trial of depuration in this country is presented.
Studies on the Susceptibility of Water Buffaloes to Leptospira
, C. K.
Canadian Journal of Comparative Medicine
Eight mature farming type, Taiwan, water buffaloes were inoculate with L. australis A while six received L. canicola. Before inoculation all animals were negative to the microscopic-agglutination test (agglutinationlysis test) using the above species as antigen.
No sign of clinical leptospirosis was observed although four animals developed temperatures.
Cultures made from buffalo blood, kidneys and urine and from blood of guinea pigs inoculated with kidney emulsion and urine from the inoculated buffalo were all negative for leptospiral organisms.
Blood samples drawn from the water buffalo 2, 3 and 4 weeks post inoculation were negative to the microscopic-agglutination test except for one animal. Blood from the animal taken two weeks post-inoculation was positive at 1:100 dilution with L. australis A antigen but that taken at 3 and 4 weeks was negative.
Toxicity of starch administered by mouth.
Boyd, E. M.
, S. J.
Canadian Medical Association Journal
Results 1-3 (3)
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