Enter Your Search:
Results 1-5 (5)
Go to page number:
Clear All Filters
Applied and Environmental Microbiology (1)
British Journal of Cancer (1)
Journal of Bacteriology (1)
Nucleic Acids Research (1)
Transactions of the American Ophthalmological Society (1)
Bollag, J M (1)
Calavan, E C (1)
Green, W R (1)
Javaherian, K (1)
Lee, I M (1)
Liu, H Y (1)
Liu, L F (1)
Liu, R. (1)
Liu, S Y (1)
Liu, Y P (1)
Marsden, E R (1)
Minard, R D (1)
Mudd, J B (1)
Sadeghi, M (1)
Salmon, S. E. (1)
Wong, V G (1)
Year of Publication
Asymmetric diphenol formation by a fungal laccase.
Bollag, J M
, S Y
Minard, R D
Applied and Environmental Microbiology
A laccase isolated from the fungus Rhizoctonia praticola catalyzed the cross-coupling of two differently halogenated phenols. When 2,4-dichlorophenol and 4-bromo-2-chlorophenol were incubated together with the enzyme, three dimers were formed and isolated by thin-layer chromatography. The molecular weights of these compounds were determined by mass spectrometry as 322, 410, and 366, which correspond with the respective dimers of each of the phenols and with a hybrid formed from both, tentatively assigned the structure 3,3',5'-trichloro-5-bromo-2,2'-diphenol. Gas chromatography-mass spectrometry analysis of these products and of their methylated derivatives lent support to these structural assignments.
Direct "wet" staining of tumour or haematopoietic colonies in agar culture.
Salmon, S. E.
British Journal of Cancer
Comparison of the membrane composition of Spiroplasma citri and the corn stunt Spiroplasma.
Mudd, J B
Lee, I M
, H Y
Calavan, E C
Journal of Bacteriology
Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenine dinucleotide diaphorase, and adenosine triphosphatase.
Experimental intraocular malignancy: the effect of intracameral perfusion.
Wong, V G
Green, W R
, Y P
Marsden, E R
Transactions of the American Ophthalmological Society
Transplantable Brown-Pearce carcinoma was adapted successfully in the rabbit anterior chamber. Regression of tumor growth was attained on tri-weekly perfusion of the AC with 10 micromolar of methotrexate. Tumor cyclic nucleotide phosphodiesterase (PDE) and protein activator were found to be markedly depressed during the course of chemotherapy and the PDE cAMP/cGMP ratio was similarly altered. Corroborative light and electron-microscopic studies showed specific alterations of intracellular organelles in relation to MTX and tumor cell death. These findings suggest that metabolic pathways of cyclic nucleotides are important biochemical modulators of neoplastic cells. The method of intraocular perfusion precludes systemic toxic effects and avoids compromising the animals' immunocompetence.
Nonhistone proteins HMG1 and HMG2 unwind DNA double helix.
, L F
Nucleic Acids Research
In a previous communication we have shown that both HMG1 and HMG2 nonhistone proteins change the DNA helical structure and the binding of HMG1 and HMG2 to DNA induces a net unwinding equivalent of DNA double helix (Javaherian, K.,
, L. F. and Wang, J. C. (1978) Science, 199, 1345-1346). Employing melting absorption technique, we now show that in the presence of salt HMG1 and HMG2 destabilize DNA whereas in the absence of salt, they both stabilize DNA molecules. Consequently the folded structure of HMG must play an important role in melting DNA. Furthermore, by measuring topological winding number using competition unwinding experiments, we conclude that HMG1 has a higher affinity for a single-stranded DNA relative to double-stranded DNA. These results together suggest that HMG1 and HMG2 unwind DNA double helix by local denaturation of the DNA base pairs. The net unwinding angles have been measured to be 22 degrees and 26 degrees per molecule of HMG1 and HMG2 respectively.
Results 1-5 (5)
Go to page number:
Remove citation from clipboard
Add citation to clipboard
This will clear all selections from your clipboard. Do you wish proceed?
Clipboard is full! Please remove an item and try again.
PubMed Central Canada is a service of the
Canadian Institutes of Health Research
(CIHR) working in partnership with the National Research Council's
Canada Institute for Scientific and Technical Information
in cooperation with the
National Center for Biotechnology Information
U.S. National Library of Medicine
(NCBI/NLM). It includes content provided to the
PubMed Central International archive
by participating publishers.