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1.  Virulence Determinants in the PB2 Gene of a Mouse-Adapted H9N2 Virus 
Journal of Virology  2014;89(1):877-882.
The molecular bases of adaptation and pathogenicity of H9N2 influenza virus in mammals are largely unknown. Here, we show that a mouse-adapted PB2 gene with a phenylalanine-to-leucine mutation (F404L) mainly contributes to enhanced polymerase activity, replication, and pathogenicity of H9N2 in mice and also increases the virulence of the H5N1 and 2009 pandemic H1N1 influenza viruses. Therefore, we defined a novel pathogenic determinant, providing further insights into the pathogenesis of influenza viruses in mammals.
doi:10.1128/JVI.01775-14
PMCID: PMC4301167  PMID: 25339773
2.  Complete Genome Sequence of Feline Panleukopenia Virus Strain HRB-CS1, Isolated from a Domestic Cat in Northeastern China 
Genome Announcements  2015;3(2):e01556-14.
Here, we report the complete genome sequence of feline panleukopenia virus (FPLV) strain HRB-CS1, isolated from a dead domestic cat showing enteric symptoms in China in 2014. The genome of HRB-CS1 was sequenced and analyzed, which will help to understand the genetic characteristics and evolution of FPLV in China.
doi:10.1128/genomeA.01556-14
PMCID: PMC4384158  PMID: 25814618
3.  Complete Genome Sequence of Feline Calicivirus Strain HRB-SS from a Cat in Heilongjiang Province, Northeastern China 
Genome Announcements  2014;2(5):e00698-14.
Here, we report the complete genome sequence of feline calicivirus (FCV) strain HRB-SS isolated in 2014 from a cat in Heilongjiang Province of northeastern China. The virus genome is 7,705 bases, excluding the poly(A) tail. This analysis will help to understand the genetic characteristics and evolution of FCV in China.
doi:10.1128/genomeA.00698-14
PMCID: PMC4155582  PMID: 25189577
4.  Coinfection of Dermacentor silvarum Olenev (Acari: Ixodidae) by Coxiella-Like, Arsenophonus-Like, and Rickettsia-Like Symbionts 
We report that multiple symbionts coexist in Dermacentor silvarum. Based on 16S rRNA gene sequence analyses, we prove that Coxiella-like and Arsenophonus-like symbionts, with 95.6% and 96.7% sequence similarity to symbionts in the closest taxon, respectively, are novel. Moreover, we also provide evidence that the Coxiella-like symbiont appears to be the primary symbiont.
doi:10.1128/AEM.03575-12
PMCID: PMC3623253  PMID: 23354701
5.  Complete Genome Sequence of a GII.17 Norovirus Isolated from a Rhesus Monkey in China 
Genome Announcements  2016;4(5):e00904-16.
The previously silent GII.17 norovirus was found to be the predominant genotype causing major epidemics in China in the 2014–2015 winter epidemic season. We report here the complete genomic sequence of a GII.17 norovirus (mky/GII.17/KM1509/CHN/2015) that infected rhesus monkeys at a monkey farm in southwestern China.
doi:10.1128/genomeA.00904-16
PMCID: PMC5017216  PMID: 27609911
6.  Development of an Enzyme-Linked Immunosorbent Assay Based on Fusion VP2332-452 Antigen for Detecting Antibodies against Aleutian Mink Disease Virus 
Journal of Clinical Microbiology  2016;54(2):439-442.
For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452 ELISA may be a preferable method for detecting antibodies against AMDV.
doi:10.1128/JCM.02625-15
PMCID: PMC4733201  PMID: 26582828
7.  Changes in the Length of the Neuraminidase Stalk Region Impact H7N9 Virulence in Mice 
Journal of Virology  2016;90(4):2142-2149.
The neuraminidase stalk of the newly emerged H7N9 influenza virus possesses a 5-amino-acid deletion. This study focuses on characterizing the biological functions of H7N9 with varied neuraminidase stalk lengths. Results indicate that the 5-amino-acid deletion had no impact on virus infectivity or replication in vitro or in vivo compared to that of a virus with a full-length stalk, but enhanced virulence in mice was observed for H7N9 encoding a 19- to 20-amino-acid deletion, suggesting that N9 stalk length impacts virulence in mammals, as N1 stalk length does.
doi:10.1128/JVI.02553-15
PMCID: PMC4734013  PMID: 26656694
8.  Unidirectionally progressive resection of lower left lung carcinoma under video-associated thoracoscopy 
Journal of Thoracic Disease  2015;7(12):2371-2375.
General anesthesia is adopted through double-lumen endotracheal intubation, one-lung ventilation on the contralateral, and intravenous injection. The patient took a 90 degree decubitus on his contralateral side. The operative incisions: the observation port was made in the mid-axillary line of the 7th intercostal section, a second horizontal incision of 4 cm as the main operation port at the 4th intercostal space between the anterior axillary line and the midclavicular line, and a 3rd incision of 1.5 cm as the secondary operation hole at the 9th intercostal space between the axillary line and the bottom scapular line. The surgeons were on the ventral side of the patient, and operated with endoscope apparatus in front of the monitor screen.
doi:10.3978/j.issn.2072-1439.2015.12.49
PMCID: PMC4703662  PMID: 26793362
Video-assisted thoracic surgery (VATS); lung adenocarcinoma; lower left lobe lobectomy
9.  Identification of novel microtubule-binding proteins by taxol-mediated microtubule stabilization and mass spectrometry analysis 
Thoracic Cancer  2015;6(5):649-654.
Microtubule-binding proteins (MBPs) are structurally and functionally diverse regulators of microtubule-mediated cellular processes. Alteration of MBPs has been implicated in the pathogenesis of human diseases, including cancer. MBPs can stabilize or destabilize microtubules or move along microtubules to transport various cargoes. In addition, MBPs can control microtubule dynamics through direct interaction with microtubules or coordination with other proteins. To better understand microtubule structure and function, it is necessary to identify additional MBPs. In this study, we isolated microtubules and MBPs from mammalian cells by a taxol-based method and then profiled a panel of MBPs by mass spectrometry. We discovered a number of previously uncharacterized MBPs, including several membrane-associated proteins and proteins involved in post-translational modifications, in addition to several structural components. These results support the notion that microtubules have a wide range of functions and may undergo more exquisite regulation than previously recognized.
doi:10.1111/1759-7714.12284
PMCID: PMC4567012  PMID: 26445615
Mass spectrometry; microtubule; microtubule-binding protein; taxol; tubulin
10.  Antibacterial Properties of Magnesium In Vitro and in an In Vivo Model of Implant-Associated Methicillin-Resistant Staphylococcus aureus Infection 
Antimicrobial Agents and Chemotherapy  2014;58(12):7586-7591.
Periprosthetic infection remains a challenging clinical complication. We investigated the antibacterial properties of pure (99.9%) magnesium (Mg) in vitro and in an in vivo rat model of implant-related infection. Mg was highly effective against methicillin-resistant Staphylococcus aureus-induced osteomyelitis and improved new peri-implant bone formation. Bacterial icaA and agr RNAIII transcription levels were also assessed to characterize the mechanism underlying the antibacterial properties of the Mg implant.
doi:10.1128/AAC.03936-14
PMCID: PMC4249557  PMID: 25288077
11.  In Vitro Potential of Lycosin-I as an Alternative Antimicrobial Drug for Treatment of Multidrug-Resistant Acinetobacter baumannii Infections 
Antimicrobial Agents and Chemotherapy  2014;58(11):6999-7002.
The resistance of multidrug-resistant Acinetobacter baumannii (MDRAB) isolates to most traditional antibiotics results in huge challenges for infection therapy. We investigated the in vitro activities of both l- and d-lycosin-I against MDRAB. These two compounds displayed high antibacterial activities and rapid bactericidal effects against MDRAB. Moreover, the compounds retained their activity even at high salt (Mg2+ or Ca2+) concentrations. These results demonstrate the potential of lycosin-I to be developed as a new antibiotic.
doi:10.1128/AAC.03279-14
PMCID: PMC4249444  PMID: 25199777
12.  Discovery of a Potent, Covalent BTK Inhibitor for B-Cell Lymphoma 
ACS Chemical Biology  2014;9(5):1086-1091.
BTK is a member of the TEC family of non-receptor tyrosine kinases whose deregulation has been implicated in a variety of B-cell-related diseases. We have used structure-based drug design in conjunction with kinome profiling and cellular assays to develop a potent, selective, and irreversible BTK kinase inhibitor, QL47, which covalently modifies Cys481. QL47 inhibits BTK kinase activity with an IC50 of 7 nM, inhibits autophosphorylation of BTK on Tyr223 in cells with an EC50 of 475 nM, and inhibits phosphorylation of a downstream effector PLCγ2 (Tyr759) with an EC50 of 318 nM. In Ramos cells QL47 induces a G1 cell cycle arrest that is associated with pronounced degradation of BTK protein. QL47 inhibits the proliferation of B-cell lymphoma cancer cell lines at submicromolar concentrations.
doi:10.1021/cb4008524
PMCID: PMC4027949  PMID: 24556163
13.  Statistical characteristics of reset switching in Cu/HfO2/Pt resistive switching memory 
A major challenge of resistive switching memory (resistive random access memory (RRAM)) for future application is how to reduce the fluctuation of the resistive switching parameters. In this letter, with a statistical methodology, we have systematically analyzed the reset statistics of the conductive bridge random access memory (CBRAM) with a Cu/HfO2/Pt structure which displays bipolar switching property. The experimental observations show that the distributions of the reset voltage (Vreset) and reset current (Ireset) are greatly influenced by the initial on-state resistance (Ron) which is closely related to the size of the conductive filament (CF) before the reset process. The reset voltage increases and the current decreases with the on-state resistance, respectively, according to the scatter plots of the experimental data. Using resistance screening method, the statistical data of the reset voltage and current are decomposed into several ranges and the distributions of them in each range are analyzed by the Weibull model. Both the Weibull slopes of the reset voltage and current are demonstrated to be independent of the on-state resistance which indicates that no CF dissolution occurs before the reset point. The scale factor of the reset voltage increases with on-state resistance while that of the reset current decreases with it. These behaviors are fully in consistency with the thermal dissolution model, which gives an insight on the physical mechanism of the reset switching. Our work has provided an inspiration on effectively reducing the variation of the switching parameters of RRAM devices.
doi:10.1186/1556-276X-9-694
PMCID: PMC4493841  PMID: 26089007
RRAM; Statistics; Conductive filament; Weibull model; Thermal dissolution
14.  A Study on the Protective Effect of Silybum Marianum Extract on Hepatic Ischemia-Reperfusion Injury 
The objective of the study was to study the protective effect of Silybum marianum extract on hepatic ischemia-reperfusion injury. Rats were randomly divided into five groups; namely Silybum marianum extract high-, medium-, and low-dose protection groups, model group and control group. Hepatic ischemia-reperfusion injury model was prepared. Serum or plasma AST, ALT, MDA, TNF-α, IL-1β, IL-6 levels were measured. The results revealed that after liver injury, AST, ALT, MDA, TNF-α, IL-1β, and IL-6 levels significantly increased in succession, showing significant differences. We concluded that inflammatory cytokines participate in liver injury and that Silybum marianum extract can reduce the production of inflammatory cytokines, and thus can have a protective effect on hepatic ischemia and reperfusion.
PMCID: PMC3847421  PMID: 24311841
Silybum marianum; hepatic ischemia-reperfusion injury; protective effect
15.  Genome Sequence of Martelella sp. Strain AD-3, a Moderately Halophilic Polycyclic Aromatic Hydrocarbon-Degrading Bacterium 
Genome Announcements  2014;2(1):e01189-13.
Martelella sp. strain AD-3, enriched from a petroleum-contaminated site with high salinity, can efficiently degrade polycyclic aromatic hydrocarbons. Here, we report the 4.75-Mb genome sequence of strain AD-3 with its genetic feature of helping to remediate environmental organic pollutants.
doi:10.1128/genomeA.01189-13
PMCID: PMC3894287  PMID: 24435873
16.  Novel Variants of Clade 2.3.4 Highly Pathogenic Avian Influenza A(H5N1) Viruses, China 
Emerging Infectious Diseases  2013;19(12):2021-2024.
We characterized 7 highly pathogenic avian influenza A(H5N1) viruses isolated from poultry in China during 2009–2012 and found that they belong to clade 2.3.4 but do not fit within the 3 defined subclades. Antigenic drift in subtype H5N1 variants may reduce the efficacy of vaccines designed to control these viruses in poultry.
doi:10.3201/eid1912.130340
PMCID: PMC3840869  PMID: 24274396
influenza; avian influenza virus; clade 2.3.4; China; clade; influenza virus; flu; H5; H5N1; highly pathogenic; viruses; avian; AIV; phylogeny; chickens; birds; HPAI
17.  Transplantation of umbilical cord and bone marrow-derived mesenchymal stem cells in a patient with relapsing-remitting multiple sclerosis 
Cell Adhesion & Migration  2013;7(5):404-407.
There is currently great interest in the use of mesenchymal stem cells as a therapy for multiple sclerosis with potential to both ameliorate inflammatory processes as well as improve regeneration and repair. Although most clinical studies have used autologous bone marrow-derived mesenchymal stem cells, other sources such as allogeneic umbilical cord-derived cells may provide a more accessible and practical supply of cells for transplantation. In this case report we present the treatment of aggressive multiple sclerosis with multiple allogenic human umbilical cord-derived mesenchymal stem cell and autologous bone marrow-derived mesenchymal stem cells over a 4 y period. The treatments were tolerated well with no significant adverse events. Clinical and radiological disease appeared to be suppressed following the treatments and support the expansion of mesenchymal stem cell transplantation into clinical trials as a potential novel therapy for patients with aggressive multiple sclerosis.
doi:10.4161/cam.26941
PMCID: PMC3903683  PMID: 24192520
mesenchymal stem cell; multiple sclerosis; stem cells; MS; axon; myelination; regeneration; transplantation
18.  Full Genome Sequence of a Natural Reassortant H9N2 Avian Influenza Virus Isolated from Domestic Ducks in Jiangsu Province, China 
Genome Announcements  2013;1(4):e00463-13.
In this study, the complete genomic sequence of a novel reassortant H9N2 avian influenza virus (AIV) from domestic ducks in eastern China was reported. Phylogenetic analysis showed that seven of the eight genes were all highly homologous to the chicken-origin H9N2 viruses, whereas the PB2 gene was homologous to the human-origin H1N1 virus, which suggested that domestic ducks might play a key role in the genetic reassortment and evolution of H9N2 AIVs in eastern China.
doi:10.1128/genomeA.00463-13
PMCID: PMC3715665  PMID: 23868123
19.  Genome Sequence of a Novel H10N9 Avian Influenza Virus Isolated from Chickens in a Live Poultry Market in Eastern China 
Genome Announcements  2013;1(4):e00386-13.
An H10N9 avian influenza virus (AIV) strain, A/Chicken/Jiangsu/RD5/2013, was isolated in China. The hemagglutinin (HA) and neuraminidase (NA) genes in this strain originated from H10N1 and H7N9 AIVs, respectively, and the other genes derived from H7N3 AIVs. Sequence analysis implies that the H10N9 AIV may be an NA gene donor for the human H7N9 influenza viruses.
doi:10.1128/genomeA.00386-13
PMCID: PMC3695435  PMID: 23814107
20.  Genome Sequence of a Novel Reassortant H3N2 Avian Influenza Virus from Domestic Mallard Ducks in Eastern China 
Genome Announcements  2013;1(2):e00221-12.
The H3 subtype avian influenza virus (AIV) can provide genes for human influenza virus through gene reassortment, which raises great concerns in terms of its potential threat to human health. Here, we report the complete genome sequence of a novel H3N2 AIV isolated from domestic ducks in the Jiangsu province of eastern China in 2004, which is a natural recombinant virus whose genes are derived from H3N8, H5N1, H5N2, H11N2, H4N6, and H1N1 AIVs. This genome will help to understand the epidemiology and molecular characteristics of H3N2 influenza virus in eastern China.
doi:10.1128/genomeA.00221-12
PMCID: PMC3624687  PMID: 23580713
21.  Risk Perceptions for Avian Influenza Virus Infection among Poultry Workers, China  
Emerging Infectious Diseases  2013;19(2):313-316.
To determine risk for avian influenza virus infection, we conducted serologic surveillance for H5 and H9 subtypes among poultry workers in Beijing, China, 2009–2010, and assessed workers’ understanding of avian influenza. We found that poultry workers had considerable risk for infection with H9 subtypes. Increasing their knowledge could prevent future infections.
doi:10.3201/eid1902.120251
PMCID: PMC3563274  PMID: 23343592
Avian influenza; influenza; viruses; poultry workers; serologic survey; knowledge; attitudes; practices
22.  Correlation between imaging features of Pneumocystis Jiroveci Pneumonitis (PCP), CD4+ T lymphocyte count, and plasma HIV viral load: A study in 50 consecutive AIDS patients 
Purpose
To investigate the imaging manifestations of Pneumocystis Jiroveci Pneumonitis (PCP) in AIDS patients, and the correlation between imaging features, CD4+ lymphocyte count, and plasma HIV viral load.
Materials and methods
A total of consecutive 50 AIDS patients with PCP were reviewed retrospectively. Chest CT manifestations, CD4+ lymphocyte count, and plasma HIV viral load were analyzed to investigate their correlation.
Results
PCP chest CT manifestations included ground-glass opacities dominated in 28 cases (28/50, 56%), lung cysts dominated in 10 cases (10/50, 20%), consolidation dominated in 6 cases (6/50, 12%), interstitial lesion dominated in 3 cases (3/50, 6%), and mixed lesions in 3 cases (3/50, 6%). In these 50 patients, CD4+ lymphocyte count ranged from 2 to 373 cells/µL. Plasma HIV viral load ranged from 500 to 5.28×107 copies/mL. CD4+ lymphocyte count in ground-glass opacities dominated patients was higher than that of lung cyst dominated patients (P<0.05). Plasma virus load of lung cysts dominated PCP patients was higher than that of consolidation dominated patients (P<0.05).
Conclusions
The typical chest imaging features of PCP in AIDS patients included lung ground-glass opacities and lung cysts. The chest imaging features were correlated with CD4+ T lymphocyte count and plasma HIV viral load.
doi:10.3978/j.issn.2223-4292.2012.05.04
PMCID: PMC3508599  PMID: 23256070
Acquired Immunodeficiency Syndrome (AIDS); Pneumocystis Jiroveci Pneumonitis; Tomography; X-ray computed; CD4+ lymphocyte counts; Virus load
23.  Protective actions of salvianolic acid A on hepatocyte injured by peroxidation in vitro 
doi:10.3748/wjg.v6.i3.402
PMCID: PMC4688763  PMID: 11819609
Salvianolic acid A; hepatocyte; carbon tetrachloride; liver injury; lipid peroxidation; water soluble vitamin E; ALT; AST; superoxide dismutase; malondiadehyde; catalase; lactase dehydrogenase; glutathione peroxidase; glutathione
24.  Clinical significance of Fas antigen expression in gastric carcinoma 
doi:10.3748/wjg.v5.i1.90
PMCID: PMC4688514  PMID: 11819398
stomach neoplasms/patholo-gy; Fas antigen; lymphatic metastasis; prognosis; immunohistochemistry
25.  Complete Sequence of the FII Plasmid p42-2, Carrying blaCTX-M-55, oqxAB, fosA3, and floR from Escherichia coli 
We sequenced a novel conjugative multidrug resistance IncF plasmid, p42-2, isolated from Escherichia coli strain 42-2, previously identified in China. p42-2 is 106,886 bp long, composed of a typical IncFII-type backbone (∼54 kb) and one distinct acquired DNA region spanning ∼53 kb, harboring 12 antibiotic resistance genes [blaCTX-M-55, oqxA, oqxB, fosA3, floR, tetA(A), tetA(R), strA, strB, sul2, aph(3′)-II, and ΔblaTEM-1]. The spread of these multidrug resistance determinants on the same plasmid is of great concern and, because of coresistance to antibiotics from different classes, is therapeutically challenging.
doi:10.1128/AAC.00475-16
PMCID: PMC4914668  PMID: 27067314

Results 1-25 (986)