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15.  P02-08. Enhancement of HIV-1 DNA vaccine immunogenicity by BCG-PSN, a novel adjuvant 
Sun, J | Liu, Y | Li, D | Hou, J | Xu, Z | Fan, W | Fu, J | Liu, Y | Shao, Y
Retrovirology  2009;6(Suppl 3):P13.
PMCID: PMC2767615
16.  Short- and long-term effects from the communication between carbon-irradiated cancer cells and non-irradiated human cells: implication for radiotherapy and space radiation 
Journal of Radiation Research  2014;55(Suppl 1):i52-i53.
Heavy ions are one of the most effective treatment for cancer patient and the common types of radiation in space, such as carbon ions [ 1, 2]. However, the mechanism underlying the response between carbon-irradiated cancer cells and neighboring bystander normal cells remains unclear. Using the layered tissue culture strategy [ 3], human glioblastoma (T98G) cells were exposed to high linear energy transfer (LET) carbon ions (LET ∼76 keV/µm) with a single dose of 6 Gy or dose divided into three fractions given at consecutive day (2 Gy × 3 days) at Heavy Ion Medical Accelerator in Chiba. Within 10 min after irradiation, carbon-irradiated T98G cells were trypsinzied and co-cultured with human skin fibroblasts (NB1RGB) in the presence or absence of gap junction inhibitor (18-α-glycyrrhetinic: AGA). During this time, irradiated T98G cells and bystander NB1RGB cells were grown on either side of an insert with 3 µm pores. Following 4 h post-irradiation, bystander NB1RGB cells were then harvested and assayed for clonogenic survival and micronucleus (MN) formation or allowed to grow for 10 weeks and assayed for MN formation. Relative to control, both single- and fractionation irradiation showed that the bystander NB1RGB cells that co-cultured with carbon-irradiated T98G cells exhibited reduced cloning efficiency and associated with increased MN formation. In contrast, treatment with AGA showed a significant increase in survival and decrease in MN formation in bystander cells. These indicated that the role of gap junction intercellular communication (GJIC) in the propagation of bystander response from the communication between carbon-irradiated cancer cells and bystander normal cells. Furthermore, the progeny of bystander cells that were co-cultured with carbon-irradiated T98G cells for 10 weeks showed that there was induction of MN formation in both cases. Interestingly, the level of MN formation is reduced if there is the inhibition of GJIC. This supports that a role of GJIC in the propagation of stressful effects in the progeny of bystander cells. Our results provide the clear evidence that the bystander effect and genomic instability depend on intercellular communication and GJIC may be a critical mediator in the observed effects.
PMCID: PMC3941514  PMID: 24585991
gap junction intercellular communication; bystander effect; genomic instability; heavy ion radiotherapy; space radiation
18.  Our strategy to achieve and document reproducible computing 
BMC Bioinformatics  2013;14(Suppl 17):A19.
PMCID: PMC3853654
19.  Feature selection and prediction with a Markov blanket structure learning algorithm 
BMC Bioinformatics  2013;14(Suppl 17):A3.
PMCID: PMC3853162
20.  A powerful association for comorbidity analysis based on score based test 
BMC Bioinformatics  2013;14(Suppl 17):A4.
PMCID: PMC3853221
23.  Effect of the pregnant mice with Graves’ disease on thyroid function in the offspring 
PMCID: PMC3850494
Graves' disease; animal model; thyroid autoantibodies; breast milk; offspring; thyroid function

Results 1-25 (248)