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1.  Practical applications of a monoclonal antibody (NDOG2) against placental alkaline phosphatase in ovarian cancer. 
A monoclonal antibody (NDOG2) against placental alkaline phosphatase (PLAP) in ovarian cancer has been used in three ways by the Bristol University Department of Obstetrics & Gynaecology. First, in an indirect immunoperoxidase technique, NDOG2 demonstrated positive standing in 64% of 56 ovarian carcinomas as well as in 25% of 44 benign tumours. The majority of these positive tumours were serous cystadenocarcinomas or serous cystadenomas and there was considerable variation in the expression of this antigen from tumour to tumour. NDOG2 was also used as the basis of two serum assays and, when labelled with 123-iodine (123I), in radioimmunoscintigraphy (RIS) to monitor patients' response to therapy. The first serum assay measures the enzymic activity of PLAP and the second recognizes the antigenicity of the molecules. Assay 2 proved more useful in that it predicted the course of the disease in 45% of patients followed up, whereas Assay 1 was only of use in 25% of cases. RIS proved to be a useful imaging technique and was at least as sensitive as conventional imaging techniques. The common causes of false-positive and false-negative results are described.
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PMCID: PMC1289996  PMID: 3906119
2.  Reduced microfilament organization in adenovirus type 5-infected rat embryo cells: a function of early region 1a. 
Journal of Virology  1985;55(3):644-650.
The actin microfilament organization in rat embryo cells was examined by fluorescence microscopy with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and by electron microscopy, after mock infection or infection with adenovirus type 5 (Ad5). Infected cells showed severely reduced numbers of actin microfilaments and stress fibers, detectable early after infection. Mutants defective in Ad5 early genes were used to show that reduced microfilament organization was a function of the Ad5 transformation early gene 1a (E1a) and did not require expression of any other viral gene. The product of the E1a 13s mRNA was essential for the effect, although the 12s mRNA product appeared to contribute. Ad5 infection of the cells had no observable effect on total cell actin levels or on the ratio of monomeric to polymeric actin. E1a, therefore, affected only the higher-order organization of actin.
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PMCID: PMC255031  PMID: 2991587
4.  Parents' understanding of coeliac disease and diet. 
Archives of Disease in Childhood  1985;60(7):672-674.
Parents of 50 children with coeliac disease were questioned about their knowledge of the disorder and the child's dietary compliance. Twenty of 50 parents admitted that compliance was less than strict. This correlated with poor knowledge of the disease which in turn was related to the family's social status. Membership of the Coeliac Society (23 of 50) correlated with both parental understanding and dietary compliance. Greater emphasis on dietary compliance and education of parents and patients is required.
PMCID: PMC1777284  PMID: 4026368
5.  Economics of an aging population. 
Throughout this century, as in many other countries, the proportion of the British population in the older age groups has increased. The effect this has on the economy is discussed. Topics covered include the determinants of the economic status of old people; the reasons for the choice of retirement ages; the burden of the aged on younger generations; the costs of pension schemes; the disincentive effects of pensions on savings; and poverty in old age.
PMCID: PMC1052414  PMID: 4009105

Results 1-6 (6)