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Clinical and Vaccine Immunology : CVI (1)
The American Journal of Tropical Medicine and Hygiene (1)
Aleixo, José Antonio Guimarães (1)
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Highly Virulent Leptospira borgpetersenii Strain Characterized in the Hamster Model
Diniz, Juliana Alcoforado
Félix, Samuel Rodrigues
Seixas Neto, Amilton Clair Pinto
Vasconcellos, Flávia Aleixo
Grassmann, André Alex
Aleixo, José Antonio Guimarães
da Silva, Éverton Fagonde
The American Journal of Tropical Medicine and Hygiene
A recent study by our group reported the isolation and partial serological and molecular characterization of four Leptospira borgpetersenii serogroup Ballum strains. Here, we reproduced experimental leptospirosis in golden Syrian hamsters (Mesocricetus auratus) and carried out standardization of lethal dose 50% (LD50) of one of these strains (4E). Clinical disease features and histopathologic analyses of tissue lesions were also observed. As results, strain 4E induced lethality in the hamster model with inocula lower than 10 leptospires, and histopathological examination of animals showed typical lesions found in severe leptospirosis. Gross pathological findings were peculiar; animals that died early had more chance of presenting severe jaundice and less chance of presenting pulmonary hemorrhages (P < 0.01). L. borgpetersenii serogroup Ballum has had a considerable growth in human leptospirosis cases in recent years. This strain has now been thoroughly characterized and can be used in more studies, especially evaluations of vaccine candidates.
Subunit Approach to Evaluation of the Immune Protective Potential of Leptospiral Antigens ▿
Félix, Samuel R.
Hartwig, Daiane D.
Argondizzo, Ana Paula C.
Silva, Éverton F.
Seixas, Fabiana K.
Neto, Amilton C. P. Seixas
Medeiros, Marco A.
Clinical and Vaccine Immunology : CVI
Leptospirosis is the most widespread zoonosis in the world. Current vaccines are based on whole-cell preparations that cause severe side effects and do not induce satisfactory immunity. In light of the leptospiral genome sequences recently made available, several studies aimed at identification of protective recombinant immunogens have been performed; however, few such immunogens have been identified. The aim of this study was to evaluate 27 recombinant antigens to determine their potential to induce an immune response protective against leptospirosis in the hamster model. Experiments were conducted with groups of female hamsters immunized with individual antigen preparations. Hamsters were then challenged with a lethal dose of Leptospira interrogans. Thirteen antigens induced protective immune responses; however, only recombinant proteins LIC10325 and LIC13059 induced significant protection against mortality. These results have important implications for the development of an efficacious recombinant subunit vaccine against leptospirosis.
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