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author:("Cui, shengmui")
1.  Identification and Characterization of Shiga Toxin Type 2 Variants in Escherichia coli Isolates from Animals, Food, and Humans▿  
Applied and Environmental Microbiology  2008;74(18):5645-5652.
There is considerable heterogeneity among the Shiga toxin type 2 (Stx2) toxins elaborated by Shiga toxin-producing Escherichia coli (STEC). One such Stx2 variant, the Stx2d mucus-activatable toxin (Stx2dact), is rendered more toxic by the action of elastase present in intestinal mucus, which cleaves the last two amino acids of the A2 portion of the toxin A subunit. We screened 153 STEC isolates from food, animals, and humans for the gene encoding Stx2dact by using a novel one-step PCR procedure. This method targeted the region of stx2dact that encodes the elastase recognition site. The presence of stx2dact was confirmed by DNA sequencing of the complete toxin genes. Seven STEC isolates from cows (four isolates), meat (two isolates), and a human (one isolate) that carried the putative stx2dact gene were identified; all were eae negative, and none was the O157:H7 serotype. Three of the isolates (CVM9322, CVM9557, and CVM9584) also carried stx1, two (P1332 and P1334) carried stx1 and stx2c, and one (CL-15) carried stx2c. One isolate, P1130, harbored only stx2dact. The Vero cell cytotoxicities of supernatants from P1130 and stx1 deletion mutants of CVM9322, CVM9557, and CVM9584 were increased 13- to 30-fold after treatment with porcine elastase. Thus, Stx2dact-producing strains, as detected by our one-step PCR method, can be isolated not only from humans, as previously documented, but also from food and animals. The latter finding has important public health implications based on a recent report from Europe of a link between disease severity and infection with STEC isolates that produce Stx2dact.
doi:10.1128/AEM.00503-08
PMCID: PMC2547040  PMID: 18658282
2.  Ciprofloxacin-Resistant Salmonella enterica Serotype Typhimurium, China 
Emerging Infectious Diseases  2008;14(3):493-495.
We characterized 44 Salmonella enterica serotype Typhimurium isolates from Tongji Hospital outpatients in Wuhan, China, May 2002–October 2005. All 31 ciprofloxacin-resistant isolates were also resistant to >8 other antimicrobial drugs and carried >2 mutations in GyrA and 1 mutation in ParC. Class 1 integrons were identified in 37 isolates.
doi:10.3201/eid1403.070857
PMCID: PMC2570801  PMID: 18325271
Salmonella Typhimurium; outpatients; ciprofloxacin; resistance; dispatch

Results 1-2 (2)