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1.  The yggH Gene of Escherichia coli Encodes a tRNA (m7G46) Methyltransferase 
Journal of Bacteriology  2003;185(10):3238-3243.
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-l-methionine-dependent formation of N7-methylguanosine at position 46 (m7G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m7G46) methyltransferase activity.
doi:10.1128/JB.185.10.3238-3243.2003
PMCID: PMC154064  PMID: 12730187

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