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1.  Genomic Screening with RNAi: Results and Challenges 
RNA interference (RNAi) is an effective tool for genome-scale, high-throughput analysis of gene function. In the past five years, a number of genome-scale RNAi high-throughput screens (HTSs) have been done in both Drosophila and mammalian cultured cells to study diverse biological processes, including signal transduction, cancer biology, and host cell responses to infection. Results from these screens have led to the identification of new components of these processes and, importantly, have also provided insights into the complexity of biological systems, forcing new and innovative approaches to understanding functional networks in cells. Here, we review the main findings that have emerged from RNAi HTS and discuss technical issues that remain to be improved, in particular the verification of RNAi results and validation of their biological relevance. Furthermore, we discuss the importance of multiplexed and integrated experimental data analysis pipelines to RNAi HTS.
doi:10.1146/annurev-biochem-060408-092949
PMCID: PMC3564595  PMID: 20367032
bioinformatics; cell biology; Drosophila; high-throughput screening
2.  Realizing the Promise of RNAi High Throughput Screening 
Developmental cell  2010;18(4):506-507.
Recently reporting in Nature, Collinet et al. describes the application of quantitative multiparametric methods to a genome-wide RNAi screen for regulators of endocytosis. The study illustrates the power of this approach beyond the identification of new endocytic components to providing insights into the design principles of the endocytic system.
doi:10.1016/j.devcel.2010.04.005
PMCID: PMC2868582  PMID: 20412765

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