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1.  Cell shape and the microenvironment regulate nuclear translocation of NF-κB in breast epithelial and tumor cells 
Molecular Systems Biology  2015;11(3):0790.
Although a great deal is known about the signaling events that promote nuclear translocation of NF-κB, how cellular biophysics and the microenvironment might regulate the dynamics of this pathway is poorly understood. In this study, we used high-content image analysis and Bayesian network modeling to ask whether cell shape and context features influence NF-κB activation using the inherent variability present in unperturbed populations of breast tumor and non-tumor cell lines. Cell–cell contact, cell and nuclear area, and protrusiveness all contributed to variability in NF-κB localization in the absence and presence of TNFα. Higher levels of nuclear NF-κB were associated with mesenchymal-like versus epithelial-like morphologies, and RhoA-ROCK-myosin II signaling was critical for mediating shape-based differences in NF-κB localization and oscillations. Thus, mechanical factors such as cell shape and the microenvironment can influence NF-κB signaling and may in part explain how different phenotypic outcomes can arise from the same chemical cues.
doi:10.15252/msb.20145644
PMCID: PMC4380925  PMID: 25735303
Bayesian; breast cancer; morphology; NF-κB; RhoA
2.  A direct look at RNAi screens 
doi:10.1038/msb.2012.14
PMCID: PMC3397413  PMID: 22531120

Results 1-2 (2)