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Briefings in Functional Genomics (1)
Developmental dynamics : an official publication of the American Association of Anatomists (1)
Esquela-Kerscher, Aurora (2)
Jo, Jeanyoung (1)
Schulman, Betsy R. Maller (1)
Slack, Frank J. (1)
Year of Publication
A growing molecular toolbox for the functional analysis of microRNAs in Caenorhabditis elegans
Briefings in Functional Genomics
With the growing number of microRNAs (miRNAs) being identified each year, more innovative molecular tools are required to efficiently characterize these small RNAs in living animal systems. Caenorhabditis elegans is a powerful model to study how miRNAs regulate gene expression and control diverse biological processes during development and in the adult. Genetic strategies such as large-scale miRNA deletion studies in nematodes have been used with limited success since the majority of miRNA genes do not exhibit phenotypes when individually mutated. Recent work has indicated that miRNAs function in complex regulatory networks with other small RNAs and protein-coding genes, and therefore the challenge will be to uncover these functional redundancies. The use of miRNA inhibitors such as synthetic antisense 2′-O-methyl oligoribonucleotides is emerging as a promising in vivo approach to dissect out the intricacies of miRNA regulation.
microRNA; Caenorhabditis elegans; miRNA inhibitors; antisense 2′-O-methyl oligoribonucleotides
Reciprocal Expression of lin-41 and the microRNAs let-7 and mir-125 During Mouse Embryogenesis
Schulman, Betsy R. Maller
Slack, Frank J.
Developmental dynamics : an official publication of the American Association of Anatomists
In C. elegans, heterochronic genes control the timing of cell fate determination during development. Two heterochronic genes, let-7 and lin-4, encode microRNAs (miRNAs) that down-regulate a third heterochronic gene lin-41 by binding to complementary sites in its 3’UTR. let-7 and lin-4 are conserved in mammals. Here we report the cloning and sequencing of mammalian lin-41 orthologs. We find that mouse and human lin-41 genes contain predicted conserved complementary sites for let-7 and the lin-4 ortholog, mir-125, in their 3’UTRs. Mouse lin-41 (Mlin-41) is temporally expressed in developing mouse embryos, most dramatically in the limb buds. Mlin-41 is down-regulated during mid-embryogenesis at the time when mouse let-7c and mir-125 RNA levels are up-regulated. Our results suggest that mammalian lin-41 is temporally regulated by miRNAs in order to direct key developmental events such as limb formation.
microRNAs; lin-41; let-7; mir-125; developmental timing; mouse embryogenesis; expression pattern; limb development; heterochronic gene
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