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Breast Cancer : Basic and Clinical Research (1)
Microporous and mesoporous materials : the official journal of the International Zeolite Association (1)
Lei, Chenghong (2)
Yu, Yuehua (2)
Chen, Baowei (1)
Chrisler, William B. (1)
Hu, Dehong (1)
Jin, Hongjun (1)
Li, Xiaolin (1)
Liu, Jun (1)
Qi, Wen (1)
Shin, Yongsoon (1)
Xiong, Yijia (1)
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Enzymatic conversion of CO2 to bicarbonate in functionalized mesoporous silica
Microporous and mesoporous materials : the official journal of the International Zeolite Association
We report here a concept converting carbon dioxide to biocarbonate in a biomimetic nanoconfiguration. Carbonic anhydrase (CA), the fastest enzyme that can covert carbon dioxide to bicarbonate, can be spontaneously entrapped in carboxylic acid group-functionalized mesoporous silica (HOOC-FMS) with super-high loading density (up to 0.5 mg of protein/mg of FMS) in sharp contrast to normal porous silica. The binding of CA to HOOC-FMS resulted in a partial conformational change comparing to the enzyme free in solution, but it can be overcome with increased protein loading density. The higher the protein loading density, the less conformational change, hence the higher enzymatic activity and the higher enzyme immobilization efficiency (up to >60%). The released enzyme still displayed the native conformational structure and the same high enzymatic activity as that prior to the enzyme entrapment, indicating that the conformational change resulted from the electrostatic interaction of CA with HOOC-FMS was not permanent. This work may provide a new approach converting carbon dioxide to biocarbonate that can be integrated with the other part of biosynthesis process for the assimilation of carbon dioxide.
carbonic anhydrase; functionalized mesopoorous silica; enzymatic activity; carbon dioxide; biocarbonate
Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing
Chrisler, William B.
Breast Cancer : Basic and Clinical Research
Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness.
microRNA-10b; breast cancer metastasis; nanoparticles
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