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1.  Measurement of striated muscle fibre diameters using interactive computer-aided microscopy 
Journal of Clinical Pathology  1982;35(11):1268-1271.
We have measured muscle fibre diameters using two methods of interactive computer-aided microscopy. They are simple to perform, reproducible and more convenient than manual methods of measurement. The technique is of general application to histological measurement.
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PMCID: PMC497940  PMID: 7142435
2.  Measurement in jejunal biopsies by computer-aided microscopy. 
Journal of Clinical Pathology  1980;33(3):254-261.
A method of morphometric analysis of jejunal mucosa using computer-aided microscopy is described. It is based on the technique of interactive computer graphics with definition by the observer of areas and lengths for measurement, but it also includes automatic measurements based on grey-level segmentation. The analyses are performed rapidly and efficiently. The technique is applicable to the analysis of biopsy tissue from many sites.
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PMCID: PMC1146049  PMID: 7381024
3.  Reading chest radiographs for pneumoconiosis by computer. 
Computer programs for measuring simple pneumoconiosis in radiographs are described and assessed. The 36 films studied had been read by 11 skilled human observers and a 'radiological score' of pneumoconiotic severity was therefore available for each film. The computer assigns to each square grid of side 3-6 mm a measure which reflects the unevenness of the density distribution in that grid. The 'computed score' is defined as the mean diversity over all relevant grids in both lung fields. On the set of 36 films the correlation between radiological score and computed score was 0-88. By contrast, the correlation between the score assigned by a single observer and the average of the scores assigned by the other 10 was in the range 0-95 to 0-98. The program can use the computed score to classify a film into one of the four major International Labour Office (ILO) U/C categories, the success rate of this process being 80% compared with those quoted by other workers in the range 45%-65%. If the films used in this study be typical, then the program described may form the basis of an automatic method for measuring pneumoconiosis in epidemiological work.
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PMCID: PMC1008075  PMID: 1103955
4.  Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain 
Annals of the Rheumatic Diseases  2013;74(1):252-259.
Objectives
Blockade of transient receptor potential vanilloid 1 (TRPV1) with systemic antagonists attenuates osteoarthritis (OA) pain behaviour in rat models, but on-target-mediated hyperthermia has halted clinical trials. The present study investigated the potential for targeting TRPV1 receptors within the OA joint in order to produce analgesia.
Methods
The presence of TRPV1 receptors in human synovium was detected using western blotting and immunohistochemistry. In a rat model of OA, joint levels of an endogenous ligand for TRPV1, 12-hydroxy-eicosatetraenoic acid (12-HETE), were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Effects of peripheral administration of the TRPV1 receptor antagonist JNJ-17203212 on afferent fibre activity, pain behaviour and core body temperature were investigated. Effects of a spinal administration of JNJ-17203212 on dorsal horn neuronal responses were studied.
Results
We demonstrate increased TRPV1 immunoreactivity in human OA synovium, confirming the diseased joint as a potential therapeutic target for TRPV1-mediated analgesia. In a model of OA pain, we report increased joint levels of 12-HETE, and the sensitisation of joint afferent neurones to mechanical stimulation of the knee. Local administration of JNJ-17203212 reversed this sensitisation of joint afferents and inhibited pain behaviour (weight-bearing asymmetry), to a comparable extent as systemic JNJ-17203212, in this model of OA pain, but did not alter core body temperature. There was no evidence for increased TRPV1 function in the spinal cord in this model of OA pain.
Conclusions
Our data provide a clinical and mechanistic rationale for the future investigation of the therapeutic benefits of intra-articular administration of TRPV1 antagonists for the treatment of OA pain.
doi:10.1136/annrheumdis-2013-203413
PMCID: PMC4283626  PMID: 24152419
Osteoarthritis; Synovitis; Knee Osteoarthritis

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