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1.  Synthesis of c-di-GMP Analogs with Thiourea, Urea, Carbodiimide, and Guanidinium Linkages 
Organic letters  2013;16(1):158-161.
The first syntheses of neutral thiourea, urea, and carbodiimide analogs, along with two guanidinium analogs, of the bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) are reported. The key intermediate, obtained in nine steps, is a 3′-amino-5′-azido-3′,5′-dideoxy derivative. The 5′-azide serves as a masked amine from which the amine is obtained by Staudinger reduction, while the 3′-amine is converted to an isothiocyanate that, while stable to chromatography, and Staudinger conditions, nevertheless reacts well with the 5′-amine.
doi:10.1021/ol403154w
PMCID: PMC3933394  PMID: 24313312
2.  Editor’s Briefing 
doi:10.3399/bjgp14X676258
PMCID: PMC3876142  PMID: 24567548
3.  We’ve been Expecting You MR Boyd 
doi:10.3399/bjgp14X676546
PMCID: PMC3876149
4.  Editor’s Briefing 
doi:10.3399/bjgp13X675205
PMCID: PMC3839352
5.  Future Perfect 
doi:10.3399/bjgp14X682549
PMCID: PMC4240112  PMID: 25452507
6.  Flesh and Bone Francis Bacon/Henry Moore 
doi:10.3399/bjgp13X674558
PMCID: PMC3809417
7.  Your Tube 
doi:10.3399/bjgp14X682069
PMCID: PMC4220237  PMID: 25348960
8.  Editor’s Briefing 
doi:10.3399/bjgp13X673540
PMCID: PMC3782766
9.  Out-of-hours care 
doi:10.3399/bjgp13X673955
PMCID: PMC3782789  PMID: 24152471
10.  Editor’s Briefing 
doi:10.3399/bjgp13X671461
PMCID: PMC3750768  PMID: 23998810
11.  The BJGP at 60 
doi:10.3399/bjgp13X671470
PMCID: PMC3750769  PMID: 23998811
12.  Death of A Soldier: A Mother’s Story Margaret Evison 
doi:10.3399/bjgp13X671759
PMCID: PMC3750790
13.  Split Personality of a Potyvirus: To Specialize or Not to Specialize? 
PLoS ONE  2014;9(8):e105770.
Bean yellow mosaic virus (BYMV), genus Potyvirus, has an extensive natural host range encompassing both dicots and monocots. Its phylogenetic groups were considered to consist of an ancestral generalist group and six specialist groups derived from this generalist group during plant domestication. Recombination was suggested to be playing a role in BYMV's evolution towards host specialization. However, in subsequent phylogenetic analysis of whole genomes, group names based on the original hosts of isolates within each of them were no longer supported. Also, nine groups were found and designated I-IX. Recombination analysis was conducted on the complete coding regions of 33 BYMV genomes and two genomes of the related Clover yellow vein virus (CYVV). This analysis found evidence for 12 firm recombination events within BYMV phylogenetic groups I–VI, but none within groups VII–IX or CYVV. The greatest numbers of recombination events within a sequence (two or three each) occurred in four groups, three which formerly constituted the single ancestral generalist group (I, II and IV), and group VI. The individual sequences in groups III and V had one event each. These findings with whole genomes are consistent with recombination being associated with expanding host ranges, and call into question the proposed role of recombination in the evolution of BYMV, where it was previously suggested to play a role in host specialization. Instead, they (i) indicate that recombination explains the very broad natural host ranges of the three BYMV groups which infect both monocots and dicots (I, II, IV), and (ii) suggest that the three groups with narrow natural host ranges (III, V, VI) which also showed recombination now have the potential to reduce host specificity and broaden their natural host ranges.
doi:10.1371/journal.pone.0105770
PMCID: PMC4141833  PMID: 25148372
14.  Plant Virology and Next Generation Sequencing: Experiences with a Potyvirus 
PLoS ONE  2014;9(8):e104580.
Next generation sequencing is quickly emerging as the go-to tool for plant virologists when sequencing whole virus genomes, and undertaking plant metagenomic studies for new virus discoveries. This study aims to compare the genomic and biological properties of Bean yellow mosaic virus (BYMV) (genus Potyvirus), isolates from Lupinus angustifolius plants with black pod syndrome (BPS), systemic necrosis or non-necrotic symptoms, and from two other plant species. When one Clover yellow vein virus (ClYVV) (genus Potyvirus) and 22 BYMV isolates were sequenced on the Illumina HiSeq2000, one new ClYVV and 23 new BYMV sequences were obtained. When the 23 new BYMV genomes were compared with 17 other BYMV genomes available on Genbank, phylogenetic analysis provided strong support for existence of nine phylogenetic groupings. Biological studies involving seven isolates of BYMV and one of ClYVV gave no symptoms or reactions that could be used to distinguish BYMV isolates from L. angustifolius plants with black pod syndrome from other isolates. Here, we propose that the current system of nomenclature based on biological properties be replaced by numbered groups (I–IX). This is because use of whole genomes revealed that the previous phylogenetic grouping system based on partial sequences of virus genomes and original isolation hosts was unsustainable. This study also demonstrated that, where next generation sequencing is used to obtain complete plant virus genomes, consideration needs to be given to issues regarding sample preparation, adequate levels of coverage across a genome and methods of assembly. It also provided important lessons that will be helpful to other plant virologists using next generation sequencing in the future.
doi:10.1371/journal.pone.0104580
PMCID: PMC4125191  PMID: 25102175
15.  Editor’s Briefing 
doi:10.3399/bjgp13X670480
PMCID: PMC3722796  PMID: 23972162
16.  Old Battles 
doi:10.3399/bjgp14X680761
PMCID: PMC4111308  PMID: 25071028
17.  Editor’s Briefing 
doi:10.3399/bjgp13X668438
PMCID: PMC3693775  PMID: 23834860
18.  The future of primary and secondary care 
doi:10.3399/bjgp13X669400
PMCID: PMC3693793  PMID: 23834877
19.  Getting Through 
doi:10.3399/bjgp14X680353
PMCID: PMC4073702  PMID: 24982469
20.  Editor’s Briefing 
doi:10.3399/bjgp13X668014
PMCID: PMC3662421  PMID: 23735376
21.  Editor’s Briefing 
doi:10.3399/bjgp14X679909
PMCID: PMC4031985  PMID: 24868040
22.  Editor’s Briefing 
doi:10.3399/bjgp13X665161
PMCID: PMC3635546  PMID: 23643202
23.  Prime Subjects 
doi:10.3399/bjgp13X665413
PMCID: PMC3609463
24.  Effects of Introduced and Indigenous Viruses on Native Plants: Exploring Their Disease Causing Potential at the Agro-Ecological Interface 
PLoS ONE  2014;9(3):e91224.
The ever increasing movement of viruses around the world poses a major threat to plants growing in cultivated and natural ecosystems. Both generalist and specialist viruses move via trade in plants and plant products. Their potential to damage cultivated plants is well understood, but little attention has been given to the threat such viruses pose to plant biodiversity. To address this, we studied their impact, and that of indigenous viruses, on native plants from a global biodiversity hot spot in an isolated region where agriculture is very recent (<185 years), making it possible to distinguish between introduced and indigenous viruses readily. To establish their potential to cause severe or mild systemic symptoms in different native plant species, we used introduced generalist and specialist viruses, and indigenous viruses, to inoculate plants of 15 native species belonging to eight families. We also measured resulting losses in biomass and reproductive ability for some host–virus combinations. In addition, we sampled native plants growing over a wide area to increase knowledge of natural infection with introduced viruses. The results suggest that generalist introduced viruses and indigenous viruses from other hosts pose a greater potential threat than introduced specialist viruses to populations of native plants encountered for the first time. Some introduced generalist viruses infected plants in more families than others and so pose a greater potential threat to biodiversity. The indigenous viruses tested were often surprisingly virulent when they infected native plant species they were not adapted to. These results are relevant to managing virus disease in new encounter scenarios at the agro-ecological interface between managed and natural vegetation, and within other disturbed natural vegetation situations. They are also relevant for establishing conservation policies for endangered plant species and avoiding spread of damaging viruses to undisturbed natural vegetation beyond the agro-ecological interface.
doi:10.1371/journal.pone.0091224
PMCID: PMC3951315  PMID: 24621926
25.  Editor's Briefing 
doi:10.3399/bjgp13X663208
PMCID: PMC3582945

Results 1-25 (187)