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Stem Cells Translational Medicine (1)
Arya, Sunil K. (1)
Beadle, Bernadette (1)
Chen, Huaying (1)
Cooper-White, Justin J. (1)
Evoy, Stephane (1)
Glass, Nick (1)
Glass, Nick R. (1)
Naidoo, Ravendra (1)
Singh, Amit (1)
Szymanski, Christine M. (1)
Tanha, Jamshid (1)
Titmarsh, Drew M. (1)
Year of Publication
Concise Review: Microfluidic Technology Platforms: Poised to Accelerate Development and Translation of Stem Cell-Derived Therapies
Titmarsh, Drew M.
Cooper-White, Justin J.
Stem Cells Translational Medicine
Stem cells are a powerful resource for producing a variety of cell types with utility in clinically associated applications, but substantial barriers to clinical translation must be overcome to realize full clinical potential. This concise review describes how microfluidic technologies can contribute to the translation of stem cell research outcomes and provides an update on innovative research efforts in this area.
Stem cells are a powerful resource for producing a variety of cell types with utility in clinically associated applications, including preclinical drug screening and development, disease and developmental modeling, and regenerative medicine. Regardless of the type of stem cell, substantial barriers to clinical translation still exist and must be overcome to realize full clinical potential. These barriers span processes including cell isolation, expansion, and differentiation; purification, quality control, and therapeutic efficacy and safety; and the economic viability of bioprocesses for production of functional cell products. Microfluidic systems have been developed for a myriad of biological applications and have the intrinsic capability of controlling and interrogating the cellular microenvironment with unrivalled precision; therefore, they have particular relevance to overcoming such barriers to translation. Development of microfluidic technologies increasingly utilizes stem cells, addresses stem cell-relevant biological phenomena, and aligns capabilities with translational challenges and goals. In this concise review, we describe how microfluidic technologies can contribute to the translation of stem cell research outcomes, and we provide an update on innovative research efforts in this area. This timely convergence of stem cell translational challenges and microfluidic capabilities means that there is now an opportunity for both disciplines to benefit from increased interaction.
Microfluidics; Stem cells; Investigative techniques; Stem cell research; Translational medical research
Surface-immobilization of chromatographically purified bacteriophages for the optimized capture of bacteria
Arya, Sunil K.
Szymanski, Christine M.
Bacteriophages offer interesting alternatives to antibodies for the specific capture and detection of pathogenic bacteria onto biosensing surfaces. Procedures for the optimal chemical immobilization of lytic bacteriophages onto surfaces are presented. More specifically, the removal of lysate contaminants from bacteriophage suspensions by size exclusion chromatography significantly increases the resultant planar surface density of immobilized bacteriophages. E. coli T4 and Salmonella enterica serovar Typhimurium P22 phage systems seem to undergo highly heterogeneous adsorption to the surface, possibly explaining the observed phage clustering at higher surface densities. The T4 phage and its E. coli host were initially employed as a model system where we discovered an optimal planar surface density of phages for best bacterial capture: 18.9 ± 0.8 phages/μm2 capturing 18.0 ± 0.3 bacteria/100 μm2. Phage surface clustering ultimately limits the T4 phage-immobilized surface’s ability to specifically capture its host bacteria. Nevertheless, this is to our knowledge the largest surface capture density of E. coli reported using intact T4 bacteriophages. Two additional purified bacteriophage systems (P22 and Campylobacter jejuni phage NCTC 12673) were then similarly studied for their ability to capture their corresponding host bacteria (Salmonella enterica serovar Typhimurium and Campylobacter jejuni respectively) on a surface.
bacteriophage; biosensor; food contamination; pathogenic bacteria; purification; surface adsorption; virus immobilization
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