Search tips
Search criteria

Results 1-12 (12)

Clipboard (0)

Select a Filter Below

Year of Publication
1.  Intramuscular Hibernoma: A Rare Tumour in Buttock 
Hibernomas are benign tumours of brown fat that does not recur after complete excision. These tumours are found most often in adults and most commonly in thigh. Four morphologic variants of hibernoma are identified: typical, myxoid, spindle cell, and lipoma-like. The most common histologic type is typical variant. In this report, we present the clinical, morphological features and discuss the differential diagnosis of a typical variant of intramuscular hibernoma.
PMCID: PMC4525518  PMID: 26266129
Brown fat cells; Lipoblasts
2.  Mesenchymal Stem Cells and Co-stimulation Blockade Enhance Bone Marrow Engraftment and Induce Immunological Tolerance 
Organ transplantation currently requires long-term immunosuppression. This is associated with multiple complications including infection, malignancy and other toxicities. Immunologic tolerance is considered the optimal solution to these limitations.
To develop a simple and non-toxic regimen to induce mixed chimerism and tolerance using mesenchymal stem cell (MSC) in a murine model.
Wild type C57BL6 (H2Dk) and Bal/C (H2Dd) mice were used as donors and recipients, respectively. We studied to achieve tolerance to skin grafts (SG) through mixed chimerism (MC) by simultaneous skin graft and non-myeloablative donor bone marrow transplantation (DBMT) +/– MSC. All recipients received rapamycin and CTLA-4 Ig without radiation.
DBMT+MSC combined with co-stimulation blockage and rapamycin led to stable mixed chimerism, expansion of Tregs population and donor-specific skin graft tolerance. The flow cytometry analysis revealed that recipient mice developed 15%–85% chimerism. The skin allografts survived for a long time. Elimination of MSC failed to induce mixed chimerism and tolerance.
Our results demonstrate that donor-specific immune tolerance can be effectively induced by non-myeloablative DBMT-MSC combination without any additional cytoreductive treatment. This approach provides a promising and non-toxic allograft tolerance strategy.
PMCID: PMC4464279  PMID: 26082829
Organ transplantation; Immunosuppression; Infection
3.  Long-term outcomes following the surgical repair of traumatic cyclodialysis clefts 
Eye  2013;27(12):1347-1352.
To evaluate the long-term visual prognosis and intraocular pressure (IOP) control following direct and indirect cycloplexy for the surgical treatment of traumatic cyclodialysis clefts.
Retrospective consecutive case series of 17 eyes of 17 patients. All eyes showing signs of ocular hypotony were treated with either cleft cyclocryotherapy and/or direct surgical cycloplexy. Cycloplexy was performed by directly suturing the ciliary body to the scleral spur under a double-lamellar limbal-based scleral flap. The main outcome measures were IOP, best-corrected visual acuity (BCVA), and the occurrence of postoperative complications.
The cyclodialysis clefts were post-traumatic in all the 17 eyes and extended for 2.1±1.6 clock-hours (range, 0.5–6 clock-hours). The mean follow-up time was 43.7±24.6 months (range, 12–110 months). Preoperatively, the mean IOP was 6.9±4.0 mm Hg (range, 2–14 mm Hg). Postoperatively, painful reversible IOP spikes of up to 70 mm Hg developed in 13 eyes. The final mean postoperative IOP was 12.2±4.1 mm Hg with no cases of secondary glaucoma. Preoperatively, BCVA was 6/12 or better in 4 eyes (24%), which rose to 12 eyes (71%) at final follow-up. Of the 12 patients who underwent direct cycloplexy, 75% achieved a final BCVA of 6/12 or better. There were no serious complications related to direct cycloplexy, including suprachoroidal haemorrhage or endophthalmitis.
Successful cyclodialysis cleft repair can lead to a good long-term visual prognosis and stable IOP control, even in cases with a protracted history of ocular hypotony.
PMCID: PMC3869508  PMID: 23989121
cyclodialysis; cleft; glaucoma; hypotony
4.  Effect of tobacco, alcohol, and smoking habits in oral precancer with histological proven epithelial dysplasia 
To assess the effect of risk factors tobacco, alcohol, and smoking habits in oral precancer patients with epithelial dysplasia status.
Material and methods
The study sample included biopsy proven cases of 29 oral submucous fibrosis (OSMF) and 43 cases of clinical leukoplakia. Histopathological diagnosis was made from formalin fixed paraffin embedded tissues as per WHO criteria. The relevant clinical and demographic details were recorded after interviewing the patients. Risk for tobacco, alcohol and smoking was determined by logistic regression analysis by SPSS software.
Presence of epithelial dysplasia was significantly associated with tobacco in OSMF & both tobacco and smoking habits in leukoplakia. In OSMF tobacco was associated with 14.16-fold (95% CI, 1.36–147.07; p < 0.026) and in leukoplakia tobacco was associated with 4.66-fold (95% CI, 1.00–21.63; p < 0.047) and smoking was associated with 9.20-fold (95% CI, 1.65–51.28; p < 0.011) increased risk of epithelial dysplasia.
Tobacco consumption was independent risk factor for epithelial dysplasia in OSMF and both tobacco and cigarette smoking were independent factors for epithelial dysplasia in leukoplakia.
PMCID: PMC3942250  PMID: 25737859
Oral precancer; Epithelial dysplasia; OSMF; Leukoplakia; Tobacco
5.  Trends of organ donation and awareness in Ernakulam, Kerala 
BMC Proceedings  2012;6(Suppl 4):P48.
PMCID: PMC3426008
6.  Congenital myopathy caused by a novel missense mutation in the CFL2 gene 
Neuromuscular Disorders  2012;22(7):632-639.
Nemaline myopathy and myofibrillar myopathy are heterogeneous myopathies that both comprise early-onset forms. We present two sisters from a consanguineous Iraqi Kurdish family with predominant axial and limb girdle weakness. Muscle biopsies showed features of both nemaline myopathy and myofibrillar myopathy. We performed homozygosity mapping in both siblings using an Affymetrix 250K Nspl SNP array. One of the overlapping homozygous regions harbored the gene CFL2. Because a mutation in CFL2 was identified in a family with nemaline myopathy, we performed sequence analysis of the gene and a novel homozygous missense mutation in exon 2 (c.19G>A, p.Val7Met) of CFL2 was identified in both siblings. CFL2 encodes the protein cofilin-2, which plays an important role in regulation of sarcomeric actin filaments. To our knowledge, this is the second family in which a mutation in CFL2 causes an autosomal recessive form of congenital myopathy with features of both nemaline and myofibrillar myopathy. Given the clinical variability and the multitude of histological features of congenital myopathies, CFL2 sequence analysis should be considered in patients presenting with an autosomal recessive form of congenital myopathy.
PMCID: PMC3377783  PMID: 22560515
Nemaline myopathy; Congenital myopathy; CFL2; Cofilin-2; Myofibrillar myopathy
7.  The UK National Cyclodiode Laser Survey 
Eye  2010;25(2):168-173.
To evaluate current practice of transscleral diode laser cyclophotocoagulation (cyclodiode) laser treatment among consultant ophthalmologists in the United Kingdom.
A 31-question survey was emailed to all practising consultant ophthalmologists who were members of the Royal College of Ophthalmologists. All non-responders were sent a postal version of the questionnaire. This paper looked at cyclodiode practice patterns and consisted of questions on demographic data, transillumination, and power settings, factors influencing practice, post-operative care, and repeat treatment.
A total of 510 participants (53.6%) responded. A total of 180 (35.3%) responders reported performing cyclodiode laser treatment, of which 84 (46.7%) were glaucoma subspecialists (GSS). Initial median power settings used were 1500 mW and 2000 ms. The average number of applications delivered per sitting was 25.5±1.2 applications for GSS vs20.6±2.0 for non-GSS in a seeing eye (P=0.0013). In all, 65% routinely transilluminated the globe of which 78% were GSS and 52.3% were non-GSS (P=0.0009). In all, 43% of the GSS vs17% of the non-GSS lowered power settings in uveitic glaucoma (P=0.013). In blind eyes, 30% of the GSS vs12% of the non-GSS increased energy levels (P=0.0014). In all, 60% of the responders performed cyclodiode at any visual acuity, whereas 22% performed combined cyclodiode and cataract surgery.
This survey highlights a wide variation in the use of cyclodiode laser treatment amongst GSS and non-GSS. However, the most frequently used practice may not be the optimal practice. A more individualised parameter according to the condition of the eyes may optimise the outcome.
PMCID: PMC3169231  PMID: 21127509
glaucoma; raised intraocular pressure; laser coagulation; ciliary body
11.  Duane's retraction syndrome. 
PMCID: PMC506364  PMID: 6019819
PMCID: PMC510221  PMID: 18170799

Results 1-12 (12)