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author:("Hu, huisheng")
1.  Distinct Geographical Distribution of the Miscanthus Accessions with Varied Biomass Enzymatic Saccharification 
PLoS ONE  2016;11(8):e0160026.
Miscanthus is a leading bioenergy candidate for biofuels, and it thus becomes essential to characterize the desire natural Miscanthus germplasm accessions with high biomass saccharification. In this study, total 171 natural Miscanthus accessions were geographically mapped using public database. According to the equation [P(H/L| East) = P(H/L∩East)/P(East)], the probability (P) parameters were calculated on relationships between geographical distributions of Miscanthus accessions in the East of China, and related factors with high(H) or low(L) values including biomass saccahrification under 1% NaOH and 1% H2SO4 pretreatments, lignocellulose features and climate conditions. Based on the maximum P value, a golden cutting line was generated from 42°25’ N, 108°22’ E to 22°58’ N, 116°28’ E on the original locations of Miscanthus accessions with high P(H|East) values (0.800–0.813), indicating that more than 90% Miscanthus accessions were originally located in the East with high biomass saccharification. Furthermore, the averaged insolation showed high P (H|East) and P(East|H) values at 0.782 and 0.754, whereas other climate factors had low P(East|H) values, suggesting that the averaged insolation is unique factor on Miscanthus distributions for biomass saccharification. In terms of cell wall compositions and wall polymer features, both hemicelluloses level and cellulose crystallinity (CrI) of Miscanthus accessions exhibited relative high P values, suggesting that they should be the major factors accounting for geographic distributions of Miscanthus accessions with high biomass digestibility.
doi:10.1371/journal.pone.0160026
PMCID: PMC4988763  PMID: 27532636
2.  miR-206 regulates cisplatin resistance and EMT in human lung adenocarcinoma cells partly by targeting MET 
Oncotarget  2016;7(17):24510-24526.
MicroRNAs (miRNAs) play a critical role in drug resistance and epithelial-mesenchymal transition (EMT). The aims of this study were to explore the potential role of miR-206 in governing cisplatin resistance and EMT in lung cancer cells. We found that both lung adenocarcinoma A549 cisplatin-resistant cells (A549/DDP) and H1299 cisplatin-resistant cells (H1299/DDP) acquired mesenchymal features and were along with low expression of miR-206 and high migration and invasion abilities. Ectopic expression of miR-206 mimics inhibited cisplatin resistance, reversed the EMT phenotype, decreased the migration and invasion in these DDP-resistant cells. In contrast, miR-206 inhibitors increased cisplatin resistance, EMT, cell migration and invasion in non-DDP-resistant cells. Furthermore, we found that MET is the direct target of miR-206 in lung cancer cells. Knockdown of MET exhibited an EMT and DDP resistant inhibitory effect on DDP-resistant cells. Conversely, overexpression of MET in non-DDP- resistant cells produced a promoting effect on cell EMT and DDP resistance. In lung adenocarcinoma tissues, we demonstrated that low expression of miR-206 were also correlated with increased cisplatin resistance and MET expression. In addition, we revealed that miR-206 overexpression reduced cisplatin resistance and EMT in DDP-resistant cells, partly due to inactivation of MET/PI3K/AKT/mTOR signaling pathway, and subsequent downregulation of MDR1, ZEB1 and Snail expression. Finally, we found that miR-206 could also sensitize A549/DDP cells to cisplatin in mice model. Taken together, our study implied that activation of miR-206 or inactivation of its target gene pathway could serve as a novel approach to reverse cisplatin resistance in lung adenocarcinomas cells.
doi:10.18632/oncotarget.8229
PMCID: PMC5029718  PMID: 27014910
miR-206; cisplatin resistance; epithelial-mesenchymal transition (EMT); MET; lung adenocarcinoma
3.  Slight uptake of 18F-FDG on positron emission tomography in pulmonary hamartoma: A case report 
Oncology Letters  2015;10(1):430-432.
The present study reports the case of a 77-year-old female that was asymptomatic at presentation and was found to possess a lesion that was incidentally identified on a computed tomography (CT) scan. The CT scan revealed a non-homogeneous, hypodense, non-lobulated solid mass, ~1.2 cm in diameter, in the left upper lobe of the lung that demonstrated minimal contrast enhancement. The following CT scan was performed only two years later. This scan revealed that the non-homogeneous round mass had increased in size to ~1.7 cm in diameter, and possessed an irregular margin, in addition to being slightly lobulated with no calcification or fat. Combined positron emission tomography and CT revealed a lobulated mass that was ~1.9 cm in diameter, demonstrating an irregular margin with involvement of the mediastinal pleura. Slight uptake of 18F-fluorodeoxyglucose was also detected. The final histological diagnosis was pulmonary hamartoma.
doi:10.3892/ol.2015.3245
PMCID: PMC4487091  PMID: 26171045
lung neoplasms; radiological diagnosis; positron emission tomography/computed tomography; computed tomography; pulmonary hamartoma
4.  Survivin promoter-regulated oncolytic adenovirus with Hsp70 gene exerts effective antitumor efficacy in gastric cancer immunotherapy 
Oncotarget  2013;5(1):150-160.
Gene therapy is a promising adjuvant therapeutic strategy for cancer treatment. To overcome the limitations of current gene therapy, such as poor transfection efficiency of vectors, low levels of transgene expression and lack of tumor targeting, the Survivin promoter was used to regulate the selective replication of oncolytic adenovirus in tumor cells, and the heat shock protein 70 (Hsp70) gene was loaded as the anticancer transgene to generate an AdSurp-Hsp70 viral therapy system. The efficacy of this targeted immunotherapy was examined in gastric cancer. The experiments showed that the oncolytic adenovirus can selectively replicate in and lyse the Survivin-positive gastric cancer cells, without significant toxicity to normal cells. AdSurp-Hsp70 reduced viability of cancer cells and inhibited tumor growth of gastric cancer xenografts in immuno-deficient and immuno-reconstruction mouse models. AdSurp-Hsp70 produced dual antitumor effects due to viral replication and high Hsp70 expression. This therapeutic system used the Survivin promoter-regulated oncolytic adenovirus vector to mediate targeted expression of the Hsp70 gene and ensure safety and efficacy for subsequent gene therapy programs against a variety of cancers.
PMCID: PMC3960197  PMID: 24473833
Gastric cancer; Survivin gene; Heat shock protein; Oncolytic adenovirus; Gene therapy
5.  A case of pulmonary sclerosing hemangioma with low 18FDG uptake in PET 
Oncology Letters  2011;3(3):646-648.
Pulmonary sclerosing hemangioma (PSH) is a relatively rare benign neoplasm, often asymptomatic and presenting as a solitary pulmonary nodule on radiological imaging studies. In the present case report, we examined a case of PSH in a young adult female, and reviewed the literature pertaining to PSH with an emphasis on 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18FDG PET/CT) and pathology. Immunohistochemical staining was also performed to confirm the diagnosis of sclerosing hemangioma. The results revealed that the tumor cells were immunopositive for epithelial membrane antigen, thyroid transcription factor-1 and vimentin and cytoskeleton 7. The patient recovered and was discharged. Thus, 18FDG PET/CT may be used in the diagnosis of a solitary benign pulmonary nodule.
doi:10.3892/ol.2011.524
PMCID: PMC3362480  PMID: 22740968
pulmonary sclerosing hemangioma; solitary pulmonary nodule; 18FDG PET; CT
6.  Expression profiling and integrative analysis of the CESA/CSL superfamily in rice 
BMC Plant Biology  2010;10:282.
Background
The cellulose synthase and cellulose synthase-like gene superfamily (CESA/CSL) is proposed to encode enzymes for cellulose and non-cellulosic matrix polysaccharide synthesis in plants. Although the rice (Oryza sativa L.) genome has been sequenced for a few years, the global expression profiling patterns and functions of the OsCESA/CSL superfamily remain largely unknown.
Results
A total of 45 identified members of OsCESA/CSL were classified into two clusters based on phylogeny and motif constitution. Duplication events contributed largely to the expansion of this superfamily, with Cluster I and II mainly attributed to tandem and segmental duplication, respectively. With microarray data of 33 tissue samples covering the entire life cycle of rice, fairly high OsCESA gene expression and rather variable OsCSL expression were observed. While some members from each CSL family (A1, C9, D2, E1, F6 and H1) were expressed in all tissues examined, many of OsCSL genes were expressed in specific tissues (stamen and radicles). The expression pattern of OsCESA/CSL and OsBC1L which extensively co-expressed with OsCESA/CSL can be divided into three major groups with ten subgroups, each showing a distinct co-expression in tissues representing typically distinct cell wall constitutions. In particular, OsCESA1, -3 & -8 and OsCESA4, -7 & -9 were strongly co-expressed in tissues typical of primary and secondary cell walls, suggesting that they form as a cellulose synthase complex; these results are similar to the findings in Arabidopsis. OsCESA5/OsCESA6 is likely partially redundant with OsCESA3 for OsCESA complex organization in the specific tissues (plumule and radicle). Moreover, the phylogenetic comparison in rice, Arabidopsis and other species can provide clues for the prediction of orthologous gene expression patterns.
Conclusions
The study characterized the CESA/CSL of rice using an integrated approach comprised of phylogeny, transcriptional profiling and co-expression analyses. These investigations revealed very useful clues on the major roles of CESA/CSL, their potentially functional complement and their associations for appropriate cell wall synthesis in higher plants.
doi:10.1186/1471-2229-10-282
PMCID: PMC3022907  PMID: 21167079

Results 1-6 (6)