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BMC Biotechnology (1)
The Malaysian Journal of Medical Sciences : MJMS (1)
Lila, Mohd Azmi Mohd (2)
Abdullah, Jafri Malin (1)
Allaudin, Zeenathul Nazariah (1)
Bahaman, Abdul Rani (1)
Ni, Lim Shen (1)
Rezaei, Morvarid Akhavan (1)
Saad, Suria Mohd (1)
Siew, John Shia Kwong (1)
Tam, Yew Joon (1)
Tan, Joo Shun (1)
Zakaria, Hayati (1)
Year of Publication
Cell Targeting in Anti-Cancer Gene Therapy
Siew, John Shia Kwong
Saad, Suria Mohd
Ni, Lim Shen
Abdullah, Jafri Malin
The Malaysian Journal of Medical Sciences : MJMS
Gene therapy is a promising approach towards cancer treatment. The main aim of the therapy is to destroy cancer cells, usually by apoptotic mechanisms, and preserving others. However, its application has been hindered by many factors including poor cellular uptake, non-specific cell targeting and undesirable interferences with other genes or gene products. A variety of strategies exist to improve cellular uptake efficiency of gene-based therapies. This paper highlights advancements in gene therapy research and its application in relation to anti-cancer treatment.
Gene therapy; anti-cancer; cell targeting; apoptosis
Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology
Tam, Yew Joon
Allaudin, Zeenathul Nazariah
Bahaman, Abdul Rani
Tan, Joo Shun
Rezaei, Morvarid Akhavan
Cell disruption strategies by high pressure homogenizer for the release of recombinant Hepatitis B surface antigen (HBsAg) from Pichia pastoris expression cells were optimized using response surface methodology (RSM) based on the central composite design (CCD). The factors studied include number of passes, biomass concentration and pulse pressure. Polynomial models were used to correlate the above mentioned factors to project the cell disruption capability and specific protein release of HBsAg from P. pastoris cells.
The proposed cell disruption strategy consisted of a number of passes set at 20 times, biomass concentration of 7.70 g/L of dry cell weight (DCW) and pulse pressure at 1,029 bar. The optimized cell disruption strategy was shown to increase cell disruption efficiency by 2-fold and 4-fold for specific protein release of HBsAg when compared to glass bead method yielding 75.68% cell disruption rate (CDR) and HBsAg concentration of 29.20 mg/L respectively.
The model equation generated from RSM on cell disruption of P. pastoris was found adequate to determine the significant factors and its interactions among the process variables and the optimum conditions in releasing HBsAg when validated against a glass bead cell disruption method. The findings from the study can open up a promising strategy for better recovery of HBsAg recombinant protein during downstream processing.
Hepatitis B surface antigen; Cell disruption; Glass bead; High pressure homogenizer; Pichia pastoris; Recombinant protein
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