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1.  Cancerous patients and outbreak of Escherichia coli: an important issue in oncology 
The widespread of the Escherichia coli outbreak in Europe becomes an important public concern at global level. The infection can be serious and might result in death. The retrospective literature review on this specific topic is performed. In this specific brief article, the author presented and discussed on the problem of Escherichia coli infection in the cancerous patients. This is an actual important issue in medical oncology for the scenario of Escherichia coli epidemic.
doi:10.1016/S2222-1808(14)60506-5
PMCID: PMC4032035
Escherichia coli; Epidemic; Cancerous; Oncology
2.  Management of Pyogenic Discitis 
Asian Spine Journal  2014;8(3):391.
doi:10.4184/asj.2014.8.3.391
PMCID: PMC4068862  PMID: 24967056
3.  Angiotensin-converting Enzyme Genetic Polymorphism 
Arquivos Brasileiros de Cardiologia  2014;102(6):611-612.
doi:10.5935/abc.20140077
PMCID: PMC4079026
Polymorphism, Genetic; Peptidyl-Dipeptidase A
4.  Vitamin D and influenza vaccination 
doi:10.4161/hv.23847
PMCID: PMC3899164  PMID: 23419509
administration; immune; influenza; status; vitamin
5.  Tamarind seed coat extract restores reactive oxygen species through attenuation of glutathione level and antioxidant enzyme expression in human skin fibroblasts in response to oxidative stress 
Objective
To investigate the role and mechanism of tamarind seed coat extract (TSCE) on normal human skin fibroblast CCD-1064Sk cells under normal and oxidative stress conditions induced by hydrogen peroxide (H2O2).
Methods
Tamarind seed coats were extracted with boiling water and then partitioned with ethyl acetate before the cell analysis. Effect of TSCE on intracellular reactive oxygen species (ROS), glutathione (GSH) level, antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activity including antioxidant protein expression was investigated.
Results
TSCE significantly attenuated intracellular ROS in the absence and presence of H2O2 by increasing GSH level. In the absence of H2O2, TSCE significantly enhanced SOD and catalase activity but did not affected on GPx. Meanwhile, TSCE significantly increased the protein expression of SOD and GPx in H2O2-treated cells.
Conclusions
TSCE exhibited antioxidant activities by scavenging ROS, attenuating GSH level that could protect human skin fibroblast cells from oxidative stress. Our results highlight the antioxidant mechanism of tamarind seed coat through an antioxidant enzyme system, the extract potentially benefits for health food and cosmeceutical application of tamarind seed coat.
doi:10.12980/APJTB.4.2014C806
PMCID: PMC3985053
Tamarindus indica; Seed coat; Antioxidant; Reactive oxygen species; Glutathione; Antioxidant enzymes; CCD-1064Sk cells
6.  Interpretation of microscopic agglutination test for leptospirosis diagnosis and seroprevalence 
Determination of antibody titer by microscopic agglutination test (MAT) has been used as a tool for leptospirosis diagnosis. Four fold or greater rise in antibody titers between acute and convalescent sera suggests recent Leptospira infection. In addition, results obtained by MAT have been used to predict infecting serovars. However, cross reactivity among various Leptospira serovars have been reported when patient sera were tested with a battery of Leptospira serovars. This study demonstrates cross- reactivity among several Leptospira serovars when MAT was performed on leptospirosis sera. The data support a role of MAT as a tool for diagnosis. However, for information on infecting serovars, Leptospira isolation and molecular identification should be performed.
doi:10.12980/APJTB.4.2014C580
PMCID: PMC4025277
Leptospirosis; Microscopic agglutination test (MAT); Seroprevalence
7.  In vitro Staphylococcus aureus-induced oxidative stress in mice murine peritoneal macrophages: a duration-dependent approach 
Objective
To evaluate the free radical generation and status of the antioxidant enzymes in murine peritoneal macrophage during in vitro vancomycin sensitive Staphylococcus aureus (VSSA) treatment with different time interval.
Methods
Peritoneal macrophages were treated with 5×106 CFU/mL VSSA cell suspension in vitro for different time interval (1, 2, 3, 6, 12, and 24 h) and superoxide anion generation, NADPH oxidase activity, myeloperoxidase activity, nitric oxide generation, antioxidant enzyme status and components of glutathione cycle were analyzed.
Results
Superoxide anion generation, NADPH oxidase activity, myeloperoxidase activity and nitric oxide generation got peak at 3 h, indicating maximum free radical generation through activation of NADPH oxidase in murine peritoneal macrophages during VSSA infection. Reduced glutathione level, glutathione peroxidase, glutathione reductase, and glutathione-s-transferase activity were decreased significantly (P<0.05) with increasing time of VSSA infection. But the oxidized glutathione level was time dependently increased significantly (P<0.05) in murine peritoneal macrophages. All the changes in peritoneal macrophages after 3 h in vitro VSSA treatment had no significant difference.
Conclusions
From this study, it may be summarized that in vitro VSSA infection not only generates excess free radical but also affects the antioxidant status and glutathione cycle in murine peritoneal macrophages.
doi:10.12980/APJTB.4.2014B341
PMCID: PMC4025306
Antioxidant enzyme; Oxidative stress; Peritoneal macrophage; Vancomycin sensitive Staphylococcus aureus
9.  Insect gut microbiome - An unexploited reserve for biotechnological application 
Metagenomics research has been developed over the past decade to elucidate the genomes of the uncultured microorganisms with an aim of understanding microbial ecology. On the other hand, it has also been provoked by the increasing biotechnological demands for novel enzymes, antibiotic and signal mimics. The gut microbiota of insects plays crucial roles in the growth, development and environmental adaptation to the host insects. Very recently, the insect microbiota and their genomes (microbiome), isolated from insects were recognized as a major genetic resources for bio-processing industry. Consequently, the exploitation of insect gut microbiome using metagenomic approaches will enable us to find novel biocatalysts and to develop innovative strategies for identifying smart molecules for biotechnological applications. In this review, we discuss the critical footstep in extraction and purification of metagenomic DNA from insect gut, construction of metagenomic libraries and screening procedure for novel gene identification. Recent innovations and potential applications in bioprocess industries are highlighted.
doi:10.12980/APJTB.4.2014C95
PMCID: PMC4025310
Microbiome; Metagenomics; Non-cultivable microbes; 16S rRNA; Gypsy moth; Termite gut
10.  Asiatic bitter yam intoxication 
doi:10.12980/APJTB.4.2014C429
PMCID: PMC4025322
11.  High seroprevalence of bluetongue virus antibodies in goats in southeast Iran 
Objective
To describe the seroprevalence rate of bluetongue virus (BTV) in goat flocks in southeast of Iran.
Methods
The blood samples were collected randomly from herds of southeast of Iran. A total of 93 sera samples were collected between 2011 and 2012. Antibodies to BTV in sera were detected by using a commercial competitive ELISA 3 according to manufacturer's instructions.
Results
The seroprevalence rates were 67.7% for goats. Within a herd, prevalence of BTV seropositive animals ranged from 33.3% to 100.0%. All goat flocks were positive to BTV antibodies.
Conclusions
This study describes a high seroprevalence rate of BTV in goat flocks in southeast of Iran for the first time.
doi:10.12980/APJTB.4.2014B599
PMCID: PMC4025340
Seroprevalence; Bluetongue virus; Goat; Iran
14.  Myeloma and diabetes mellitus 
doi:10.4103/2278-330X.130469
PMCID: PMC4014647  PMID: 24818112
15.  Tuberculosis and lung cancer 
doi:10.4103/2278-330X.130471
PMCID: PMC4014648  PMID: 24818113
20.  Multiple Sparganosis 
Archives of Plastic Surgery  2014;41(2):181.
doi:10.5999/aps.2014.41.2.181
PMCID: PMC3961618  PMID: 24665429
21.  Discordant HIV Couple 
doi:10.4103/0019-5154.127689
PMCID: PMC3969687  PMID: 24700946
22.  Paederus Dermatitis 
doi:10.4103/0019-5154.127687
PMCID: PMC3969685  PMID: 24700944
23.  Tuberculosis of Sacrum 
Asian Spine Journal  2014;8(1):112.
doi:10.4184/asj.2014.8.1.112
PMCID: PMC3939363  PMID: 24596614
25.  Fine Needle Aspiration 
Trauma Monthly  2014;19(1):e16848.
doi:10.5812/traumamon.16848
PMCID: PMC3955927  PMID: 24719828
Diagnosis; Biopsy, Fine-Needle; Sensitivity and Specificity; Predictive Value of Tests

Results 1-25 (351)