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1.  Whole-Genome Sequence of Sungri/96 Vaccine Strain of Peste des Petits Ruminants Virus 
Genome Announcements  2014;2(1):e00056-14.
We report the complete genome sequence of the Sungri/96 vaccine strain of peste des petits ruminants virus (PPRV). The whole-genome nucleotide sequence has 89 to 99% identity with the available PPRV genome sequences in the NCBI database. This study helps to understand the epidemiological and molecular characteristics of the Sungri/96 strain.
doi:10.1128/genomeA.00056-14
PMCID: PMC3924372  PMID: 24526640
2.  Turmeric - A new treatment option for lichen planus: A pilot study 
Turmeric is dried rhizome of the perennial herbs curcumalonga. It is called Haldi in Hindi, turmeric in English, ukon in Japanese. It has been used in Asian Medicine since the second millennium BC. It's utility is referred to in the ancient Hindu script the Ayurveda. Pathogenesis of the OLP should be taken in consideration for the treatment point of view. The Cell mediated immunity to secondary antigenic change in oral mucous membrane is thought to play a major role in its pathogenesis modified keratocyte surface antigens are the primary target for cytotoxic cellular response. Curcumin also been shown to have immune modulatory effect involving activation of host macrophages and natural killer cells and modulation of lymphocytes mediated function.
doi:10.4103/0975-5950.127651
PMCID: PMC3961895  PMID: 24665176
Antioxidants; lichenplanus; turmeric
3.  Effect of walking (aerobic isotonic exercise) on physiological variants with special reference to Prameha (diabetes mellitus) as per Prakriti 
Ayu  2012;33(1):44-49.
Walking is an isotonic aerobic exercise and has been associated with reduced blood sugar level in diabetic patients and other physiological changes in observational studies. A study was conducted to determine the effect of isotonic aerobic exercise (walking) on blood sugar level and other important physiological parameters as per Prakriti. This study was carried out in Department of Kriya Sharira and Kayachikita, S. S. Hospital; Faculty of Ayurveda, IMS, BHU and some cases were enrolled from Kashi Mumukshu Bhawan, Assi, Varanasi. A total 83 (32 diabetic control and 51 diabetic exercise) diabetic cases were included with age ranging from 35 to 65 years during January 2009 to January 2011. Control cases enrolled in this study had not performed any exercise. The results of the study revealed that, there is a strong association in between Prakriti, Blood pressure and certain other Biochemical parameters.
doi:10.4103/0974-8520.100308
PMCID: PMC3456862  PMID: 23049183
Aerobic exercise; diabetes mellitus; exercise; Prakriti; walking
4.  Anti-histaminic, mast cell stabilizing and bronchodilator effect of hydroalcoholic extract of polyherbal compound– Bharangyadi 
Ancient Science of Life  2012;31(3):95-100.
Bronchial asthma is a chronic inflammatory disorder of the airways associated with reversible airway obstruction and increased airway responsiveness to a variety of stimuli. An intuitive inference from this definition is that a causal relationship may exist between airway inflammation and airway hyperresponsiveness. It can be say that “airway inflammation equal to airway hyperresponsiveness”. Attachment of antigen antibody complex to the mast cell causes its disruption and release of inflammatory mediators such as histamine.To evaluate the efficacy of anti-asthmatic property of a drug, evaluation of anti-histaminic, mast cell stabilizing and bronchodilator property can be use as pharmacodynamic parameter. Bharangyadi is a polyherbal compound having Bharangi (Clerodendrum serratum), Sati (Hedychium spicatum) and Pushkarmoola (Inula racemosa) as ingredient herbs The present study aimed to evaluate the anti-asthmatic activity of an indigenous polyherbal compound Bharangyadi through various in-vitro & in-vivo experimental models.
The results demonstrate that drug has potent histamine antagonism property with significant mast cell stabilizing and spasmolytic activity in the experimental animals. Compound 48/80, a potent mast cell degranulator, provoked 76% degranulation of mast cells in the control group. Ethanolic extract of Bharangyadi at the doses 500 and 1000 μg/ml protected from compound 48/80-evoked degranulation (P < 0.01) in dose dependent manner.
Pre-treatment with Bharangyadi extract showed 80% & 86% protection from histamine induced bronchoconstriction in guinea pigs with 27.8% and 36.1% increase in preconvoulsion time (equal to standard drug). Screening of Histamine antagonism activity on guinea pig ileum showed that drug reduces the smooth muscle contraction in dose dependent manner. Increasing concentration of Bharangyadi extract with maximum dose of histamine (1.6μg) showed maximum inhibition at the dose of 50mg (99.78%). Inhibition of smooth muscle contraction by addition of drug in organ bath before adding histamine showed that drug has preventive type antagonism.
doi:10.4103/0257-7941.103182
PMCID: PMC3530345  PMID: 23284214
Anti-asthmatic; anti-histaminic; Bharangyadi polyherbal compound; bronchodilator; Compound 48/80; mast cell stabilization
5.  Molecular Characterization of Classical swine fever virus Involved in the Outbreak in Mizoram 
Classical swine fever is the most insidious and devastating disease of pigs and wild boars. The virus is closely related to the other members of the genus Pestivirus. The outbreak recorded in Mizoram, India was strategically important as the state shares porous international boundary with East Asian countries. Both immunodiagnostic and molecular techniques were used to confirm the involvement of Classical swine fever virus (CSFV) in this outbreak. Sandwich ELISA and direct FAT could detect CSFV in the tissue samples. RT-nPCR specifically amplified E2 and 5’NTR product of 271 bp. Phylogenetic analysis showed, that the Mizoram isolate (MZ4/69) was very close to the Chinese strain Shimen-HVRI (93.0%) rather than other Indian isolate (CSF-30-03). Present study provides a valuable sequence based molecular data on Indian isolate of CSFV and will be useful in investigation on transmission of such disease from neighbour countries.
doi:10.1007/s13337-010-0006-z
PMCID: PMC3550764  PMID: 23637482
Classical swine fever virus ELISA; RT-PCR; Sequence analysis
6.  Clinical efficacy of Rasona Pinda in the management of Amavata (rheumatoid arthritis) 
Ayu  2010;31(3):280-286.
In the present clinical study, 63 patients of Amavata were registered from the Kayachikitsa out patient department/indoor patient department (OPD/IPD) of Sir Sunder Lal Hospital (Indian Medicine Wing), IMS, BHU, Varanasi-5. In group I (Rasona Pinda), 27 patients completed the study of a total of 33patients registered in the group (six patients dropped out mid–therapy). In group II (control group), 23 patients completed all three follow-ups out of 30 patients (there were seven dropouts in mid–therapy). In group I, complete remission in 29.6%, major improvement in 59.3% and minor improvement in change font so as to appear 11.1% were observed. In group II, complete remission in 13%, major improvement in 21.7%, minor improvement in 39.1% and unchanged in 26.9% of the patients were observed.
doi:10.4103/0974-8520.77149
PMCID: PMC3221059  PMID: 22131727
Amavata; Rheumatoid Arthritis; Agni; Ama; Rasona Pinda; Visual Analog Scale
7.  Role of serotonergic mechanism in gastric contractions induced by Indian Red Scorpion (Mesobuthus tamulus) venom 
Indian Journal of Pharmacology  2009;41(6):255-257.
Aim:
Gastric dysfunctions are commonly seen after scorpion envenomation, and the underlying mechanisms are not clear. Therefore, the present study was undertaken to investigate the effect of Indian red scorpion (Mesobuthus tamulus, MBT) venom on gastric fundus muscle contraction and the underlying mechanisms involved.
Materials and Methods:
In vitro isometric contraction was recorded from gastric fundus muscle strips on a chart recorder. The tissue was exposed to different concentrations of serotonin or crude MBT venom. The contractile responses to venom were expressed as the percentage of maximum contraction produced by serotonin at the beginning of each experiment. The contractile responses to 1.0 μg/ml of crude MBT venom were ascertained in the absence or presence of serotonin antagonist, methysergide.
Results:
Serotonin produced concentration-dependent fundus contractions (0.004–4.0 μM), and maximum contractile response was observed at 4.0 μM of serotonin. Hence, the contractile response obtained at 4.0 μM of serotonin was taken for normalization. The crude MBT venom (0.1–1.0 μg/ml) produced a concentration-dependent increase in fundus contractions (as % of maximum fundus contraction produced by serotonin at 4.0 μM). The maximum response was observed at 1.0 μg/ml of crude venom and a further increase in the concentration, up to 3.0 μg/ml, did not increase the response. In a separate series of experiments, pre-treatment with methysergide (1.0 μM) significantly attenuated the contractile response elicited by the venom (1.0 μg/ml) (P<0.05) and blocked the serotonin (4.0 μM) response.
Conclusion:
The results suggest that the crude MBT venom produces gastric fundus contractions by partially involving serotonin.
doi:10.4103/0253-7613.59923
PMCID: PMC2846498  PMID: 20407555
Gastric fundus contractions; Mesobuthus tamulus venom; serotonin; serotonergic mechanisms
8.  CLINICAL SURVEY OF CANNABIS USERS IN VARANASI 
Ancient Science of Life  1991;10(3):194-198.
A survey of Cannabis users reveal that long-term use of the drug does not produce any harmful effect. The findings of this scientific study is reported here minutely.
PMCID: PMC3331287  PMID: 22556533
9.  Structural features related to hydrolytic activity against ceftazidime of plasmid-mediated SHV-type CAZ-5 beta-lactamase. 
Antimicrobial Agents and Chemotherapy  1989;33(12):2160-2163.
Tryptic peptides of the novel ceftazidimase CAZ-5 were sequenced by manual Edman degradation and aligned according to strong homology (more than 98%) with SHV-1 and SHV-2 beta-lactamase sequences. CAZ-5 differed from SHV-1 by five amino acid substitutions. Unusually high activity of CAZ-5 towards ceftazidime was imputed to substitution of a Lys for a Glu at position 214 of the mature protein.
PMCID: PMC172844  PMID: 2694955
10.  EXPERIMENTAL AND CLINICAL STUDIES ON DIABETES MELLITUS EVALUATING THE EFFICACY OF AN INDIGENOUS ORAL HYPOGLYCAEMIC DRUG – ARANI (CLERODENDRON PHLOMIDIS) 
Ancient Science of Life  1984;3(4):216-224.
Diabetes mellitus is a common problem in clinical practice. An indigenous herbal drug Arani (Clerodendron phlomidis) was selected for this experimental and clinical study. The experimental study was conducted on albino rats. Arani inhibited the adrenaline induced hyperglycaemia effectively. The alcoholic extractive of Arani produced a well comparable fall in blood sugar to that of tolbutamide. Moreover, Arani caused a significant fall in hyperglycaemia ofalloxan diabetic patients along with clinical improvement. The results were found quite comparable to tolbutamide
PMCID: PMC3331572  PMID: 22557410
11.  CLINICAL TRIAL OF ADHATODA VASICA SYRUP (VASA) IN THE PATIENTS OF NON-ULCER DYSPEPSIA (AMLAPITTA) 
Ancient Science of Life  1983;3(1):19-23.
The efficacy of Vasa (Adhatoda vasica) in the form of syrup against Non-ulcer Dyspepsia (Amiapitta) through a clinical trial is attempted here. This trial showed hopeful results. The drug has reduced the total and free HCL in the patients of hyperacidity and hyperchlorhydria.
PMCID: PMC3331538  PMID: 22557371
12.  CLINICAL STUDIES ON KALMEGH (ANDROGRAPHIS PANICULATA NEES) IN INFECTIVE HEPATITIS 
Ancient Science of Life  1983;2(4):208-215.
Infective hepatitis ia an acute inflamatory condition of liver. It is usually manifested in the form of Jaundice. In this clinical study Kalmegh(Andrographis paniculata Nees) was given in the decoction form to the patients of infective hepitis. The results were assessed on the basis of clinical and biochemical parameters. A marked symptomatic improvement in majority of the cases was observed. A statistically highly significant decrease was noted in various liver function tests viz., serum bilirubin, thymol turbidity, alkaline phosphatase, S.G.O.T.; S.G.P.T. and serum globulin fraction of protein. Moreover it increased significantly total serum globulin fraction of protien. Moreover it increased significantly total serum protein level along with albumin fraction. On the total assessment 80% cases of this series were cured and 20% patients were relieved. Therefore, Kalmegh appears to be a useful remedy for the treatment of infective hepatitis.
PMCID: PMC3336768  PMID: 22556984

Results 1-13 (13)