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Aquatic Biosystems (1)
Evidence-based Complementary and Alternative Medicine : eCAM (1)
Kumar, Ameeta Ravi (2)
Bhargava, Shobha Y. (1)
Bhat, Menakshi (1)
Dam, Sushovan (1)
Dusane, Devendra H (1)
Joshi, Bimba N. (1)
Nancharaiah, Yarlagadda V (1)
Venugopalan, Vayalam P (1)
Zinjarde, Smita S (1)
Zinjarde, Smita S. (1)
Year of Publication
Disruption of Yarrowia lipolytica biofilms by rhamnolipid biosurfactant
Dusane, Devendra H
Nancharaiah, Yarlagadda V
Venugopalan, Vayalam P
Zinjarde, Smita S
Yarrowia lipolytica is an ascomycetous dimorphic fungus that exhibits biofilm mode of growth. Earlier work has shown that biosurfactants such as rhamnolipids are efficient dispersants of bacterial biofilms. However, their effectiveness against fungal biofilms (particularly Y. lipolytica) has not been investigated. The aim of this study was to determine the effect of rhamnolipid on a biofilm forming strain of Y. lipolytica. Two chemical surfactants, cetyl-trimethyl ammonium bromide (CTAB) and sodium dodecyl sulphate (SDS) were used as controls for comparison.
The methylene blue dye exclusion assay indicated an increase in fungal cell permeability after rhamnolipid treatment. Microtiter plate assay showed that the surfactant coating decreased Y. lipolytica biofilm formation by 50%. Rhamnolipid treatment disrupted pre-formed biofilms in a more effective manner than the other two surfactants. Confocal laser scanning microscopic studies showed that biofilm formation onto glass surfaces was decreased by 67% after sub-minimum inhibitory concentration (sub-MIC) treatment with rhamnolipids. The disruption of biofilms after rhamnolipid treatment was significant (P<0.05) when compared to SDS and CTAB.
The results indicate a potential application of the biological surfactant to disrupt Y. lipolytica biofilms.
Biofilm; Biosurfactant; CTAB; Rhamnolipid; SDS; Yarrowia lipolytica
Antidiabetic Indian Plants: A Good Source of Potent Amylase Inhibitors
Zinjarde, Smita S.
Bhargava, Shobha Y.
Joshi, Bimba N.
Evidence-based Complementary and Alternative Medicine : eCAM
Diabetes is known as a multifactorial disease. The treatment of diabetes (Type II) is complicated due to the inherent patho-physiological factors related to this disease. One of the complications of diabetes is post-prandial hyperglycemia (PPHG). Glucosidase inhibitors, particularly α-amylase inhibitors are a class of compounds that helps in managing PPHG. Six ethno-botanically known plants having antidiabetic property namely, Azadirachta indica Adr. Juss.; Murraya koenigii (L.) Sprengel; Ocimum tenuflorum (L.) (syn: Sanctum); Syzygium cumini (L.) Skeels (syn: Eugenia jambolana); Linum usitatissimum (L.) and Bougainvillea spectabilis were tested for their ability to inhibit glucosidase activity. The chloroform, methanol and aqueous extracts were prepared sequentially from either leaves or seeds of these plants. It was observed that the chloroform extract of O. tenuflorum; B. spectabilis; M. koenigii and S. cumini have significant α-amylase inhibitory property. Plants extracts were further tested against murine pancreatic, liver and small intestinal crude enzyme preparations for glucosidase inhibitory activity. The three extracts of O. tenuflorum and chloroform extract of M. koenigi showed good inhibition of murine pancreatic and intestinal glucosidases as compared with acarbose, a known glucosidase inhibitor.
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