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1.  Effects of slow breathing exercise on cardiovascular functions, pulmonary functions & galvanic skin resistance in healthy human volunteers - a pilot study 
Background & objectives:
Regular practice of slow breathing has been shown to improve cardiovascular and respiratory functions and to decrease the effects of stress. This pilot study was planned to evaluate the short term effects of pranayama on cardiovascular functions, pulmonary functions and galvanic skin resistance (GSR) which mirrors sympathetic tone, and to evaluate the changes that appear within a short span of one week following slow breathing techniques.
Methods:
Eleven normal healthy volunteers were randomized into Pranayama group (n=6) and a non-Pranayama control group (n=5); the pranayama volunteers were trained in pranayama, the technique being Anuloma-Viloma pranayama with Kumbhak. All the 11 volunteers were made to sit in similar environment for two sessions of 20 min each for seven days, while the pranayama volunteers performed slow breathing under supervision, the control group relaxed without conscious control on breathing. Pulse, GSR, blood pressure (BP) and pulmonary function tests (PFT) were measured before and after the 7-day programme in all the volunteers.
Results:
While no significant changes were observed in BP and PFT, an overall reduction in pulse rate was observed in all the eleven volunteers; this reduction might have resulted from the relaxation and the environment. Statistically significant changes were observed in the Pranayama group volunteers in the GSR values during standing phases indicating that regular practice of Pranayama causes a reduction in the sympathetic tone within a period as short as 7 days.
Interpretation & conclusions:
Beneficial effects of pranayama started appearing within a week of regular practice, and the first change appeared to be a reduction in sympathetic tone.
PMCID: PMC3734683  PMID: 23760377
Galvanic skin resistance; Pranayama; sympathetic tone
2.  Synthesis, Antimicrobial and cytotoxic activity of New Heterocyclic Hybrids Based on 2,5-Dimethylpyrrole and Pyrrole Scaffolds 
A series of 4-(2,5-dimethylpyrrol-1-yl)/4-pyrrol-1-yl benzoic acid hydrazide analogs, some derived triazoles, azetidinones, thiazolidinones, and pyrroles have been synthesized in good yields and structures of these compounds were established by IR, 1H NMR, 13C NMR, mass spectral, and elemental analysis. These compounds were evaluated for their preliminary in vitro antibacterial, antifungal, and antitubercular activity against Mycobacterium tuberculosis H37 Rv strain by the broth dilution assay method. Twenty one of these compounds displayed good antimicrobial activity, with a MIC value of 1-4 μg/ml. Several compounds 4c, 8-10, 15b-15h, and 16b-16d exhibited good in vitro antitubercular activity with MIC value 1-2 μg/ml. Further, some title compounds were also assessed for their cytotoxic activity (IC50) against mammalian Vero cell lines and A549 (lung adenocarcinoma) cell lines using the MTT assay method. The results revealed that these compounds exhibit antitubercular activity at non-cytotoxic concentrations.
doi:10.4103/0250-474X.117439
PMCID: PMC3783749  PMID: 24082347
Pyrroles; acid hydrazide derivatives; antibacterial activity; antitubercular activity; antifungal activity; broth dilution assay method; cytotoxicity
3.  A case of hepatomegaly 
Postgraduate Medical Journal  2007;83(984):e1-e2.
doi:10.1136/pgmj.2007.062471
PMCID: PMC2600125  PMID: 17916864
AL amyloid; Congo red; hepatomegaly
6.  Continuous, non-invasive measurement of the haemodynamic response to submaximal exercise in patients with diabetes mellitus: evidence of impaired cardiac reserve and peripheral vascular response 
Heart  2009;96(1):36-41.
Background:
Reduced exercise capacity in diabetics has been attributed to limitations in cardiac function and microvascular dysfunction leading to impaired oxygen supply and nutritive perfusion to exercising muscles.
Objective:
To study changes in cardiac function and microvascular utilisation during exercise in diabetic individuals compared to age-matched controls.
Methods:
Diabetics with glycosylated haemoglobin (HbA1c) <8 (n = 31), diabetics with HbA1c ⩾8 (n = 38) and age-matched non-diabetic controls (n = 32) performed exercise at 50 W for 10 minutes followed by recovery, with continuous monitoring of cardiac function by impedance cardiography and regional flow and oxygen saturation by laser Doppler and white light spectroscopy.
Results:
In the diabetics, cardiac reserve during exercise and cardiac overshoot during recovery are significantly reduced because of reduction in capacity to increase stroke volume. Regional flow to the exercising muscle is reduced and there is also disproportionately greater desaturation of the regional flow. Abnormalities in cardiac function and regional perfusion are related to the severity of diabetes.
Conclusion:
Cardiac response to exercise is attenuated significantly in diabetic individuals. Simultaneously, there is impairment in the regional distribution. These changes could be the harbinger of reduced exercise capacity in diabetics.
doi:10.1136/hrt.2009.177113
PMCID: PMC3272706  PMID: 19850585
7.  Arsenic accumulation by Pseudomonas stutzeri and its response to some thiol chelators 
Objective
The aim of this study is to examine arsenic accumulation by Pseudomonas stutzeri and its response to some thiol chelators, DMPS and MiADMSA.
Methods
Determination of arsenic accumulation by Pseudomonas sp. was carried out using an atomic absorption spectrophotometer, a TEM and an EDAX. Arsenate reductase enzyme assay was carried out from a cell-free extract of Pseudomonas sp. The effect of chelating agents on arsenite accumulation was analyzed. Total cellular proteins were analyzed using 1-D SDS-PAGE.
Results
Pseudomonas sp. exhibited a maximum accumulation of 4 mg As g−1 (dry weight). TEM and EDAX analysis showed the presence of As-containing electron-dense particles inside the cells. Data on arsenate reductase enzyme kinetics yielded a Km of 0.40 mM for arsenate and a Vmax of 5,952 μmol arsenate reduced per minute per milligram of protein. The chelating agents MiADMSA and DMPS were found to reduce the arsenic accumulation by 60 and 35%, respectively, whereas the presence of both chelating agents in medium containing cells pretreated with arsenite reduced it by up to 90%. The total protein profile of the cellular extract, obtained by 1-D SDS-PAGE, indicated five upregulated proteins, and three of these proteins exhibited differential expression when the cells were grown with MiADMSA and DMPS.
Conclusion
This study shows a new approach towards arsenic detoxification. A combination treatment with MiADMSA and DMPS may be useful for removing intracellular arsenic. The proteins that were found to be induced in this study may play an important role in the extrusion of arsenic from the cells, and this requires further characterization.
doi:10.1007/s12199-008-0038-9
PMCID: PMC2698249  PMID: 19568912
Arsenic bioaccumulation; Arsenate reductase; Chelating agent; Arsenic removal; Arsenic-induced protein/s
8.  An unusual case of hepatosplenomegaly 
Gut  2005;54(9):1272.
doi:10.1136/gut.2005.064824
PMCID: PMC1774646  PMID: 16099794
hepatosplenomegaly; hepatic fibrosis; intrahepatic biliary ducts; Caroli’s disease; autosomal recessive polycystic kidney disease; congenital hepatic fibrosis
9.  An unusual case of colitis 
Gut  2004;53(12):1824.
doi:10.1136/gut.2004.040451
PMCID: PMC1774320  PMID: 15542522
non-Hodgkin’s lymphoma; T cell lymphoma; ulcerative colitis
10.  Veterinary public health in the Nepal Himalaya. 
The Canadian Veterinary Journal  2000;41(11):879-881.
Images
PMCID: PMC1476451  PMID: 11126497
11.  Purification and characterization of a 1,2,4-trihydroxybenzene 1,2-dioxygenase from the basidiomycete Phanerochaete chrysosporium. 
Journal of Bacteriology  1994;176(16):4838-4844.
1,2,4-Trihydroxybenzene (THB) is an intermediate in the Phanerochaete chrysosporium degradation of vanillate and aromatic pollutants. A P. chrysosporium intracellular enzyme able to oxidatively cleave the aromatic ring of THB was purified by ammonium sulfate precipitation, hydrophobic and ion-exchange chromatographies, and native gel electrophoresis. The native protein has a molecular mass of 90 kDa and a subunit mass of 45 kDa. The enzyme catalyzes an intradiol cleavage of the substrate aromatic ring to produce maleylacetate. 18O2 incorporation studies demonstrate that molecular oxygen is a cosubstrate in the reaction. The enzyme exhibits high substrate specificity for THB; however, catechol cleavage occurs at approximately 20% of the optimal rate. THB dioxygenase catalyzes a key step in the degradation pathway of vanillate, an intermediate in lignin degradation. Maleylacetate, the product of THB cleavage, is reduced to beta-ketoadipate by an NADPH-requiring enzyme present in partially purified extracts.
Images
PMCID: PMC196317  PMID: 8050996
12.  Degradation of 2,4,5-trichlorophenol by the lignin-degrading basidiomycete Phanerochaete chrysosporium. 
Under secondary metabolic conditions the white rot basidiomycete Phanerochaete chrysosporium rapidly mineralizes 2,4,5-trichlorophenol. The pathway for degradation of 2,4,5-trichlorophenol was elucidated by the characterization of fungal metabolites and oxidation products generated by purified lignin peroxidase (LiP) and manganese peroxidase (MnP). The multistep pathway involves cycles of peroxidase-catalyzed oxidative dechlorination reactions followed by quinone reduction reactions to yield the key intermediate 1,2,4,5-tetrahydroxybenzene, which is presumably ring cleaved. In the first step of the pathway, 2,4,5-trichlorophenol is oxidized to 2,5-dichloro-1,4-benzoquinone by either MnP or Lip. 2,5-Dichloro-1,4-benzoquinone is then reduced to 2,5-dichloro-1,4-hydroquinone. The 2,5-dichloro-1,4-hydroquinone is oxidized by MnP to generate 5-chloro-4-hydroxy-1,2-benzoquinone. The orthoquinone is in turn reduced to 5-chloro-1,2,4-trihydroxybenzene. Finally, the 5-chlorotrihydroxybenzene undergoes another cycle of oxidative dechlorination and reduction reactions to generate 1,2,4,5-tetrahydroxybenzene. The latter is presumably ring cleaved, with subsequent degradation to CO2. In this pathway, the substrate is oxidatively dechlorinated by LiP or MnP in a reaction which produces a quinone. The quinone intermediate is recycled by a reduction reaction to regenerate an intermediate which is again a substrate for peroxidase-catalyzed oxidative dechlorination. This pathway apparently results in the removal of all three chlorine atoms before ring cleavage occurs.
PMCID: PMC182161  PMID: 8328802
13.  Degradation of 2,4-dinitrotoluene by the lignin-degrading fungus Phanerochaete chrysosporium. 
Under ligninolytic conditions, the white rot basidiomycete Phanerochaete chrysosporium mineralizes 2,4-dinitrotoluene (I). The pathway for the degradation of I was elucidated by the characterization of fungal metabolites and oxidation products generated by lignin peroxidase (LiP), manganese peroxidase (MnP), and crude intracellular cell extracts. The multistep pathway involves the initial reduction of I to yield 2-amino-4-nitrotoluene (II). II is oxidized by MnP to yield 4-nitro-1,2-benzoquinone (XII) and methanol. XII is then reduced to 4-nitro-1,2-hydroquinone (V), and the latter is methylated to 1,2-dimethoxy-4-nitrobenzene (X). 4-Nitro-1,2-hydroquinone (V) is also oxidized by MnP to yield nitrite and 2-hydroxybenzoquinone, which is reduced to form 1,2,4-trihydroxybenzene (VII). 1,2-Dimethoxy-4-nitrobenzene (X) is oxidized by LiP to yield nitrite, methanol, and 2-methoxy-1,4-benzoquinone (VI), which is reduced to form 2-methoxy-1,4-hydroquinone (IX). The latter is oxidized by LiP and MnP to 4-hydroxy-1,2-benzoquinone, which is reduced to 1,2,4-trihydroxybenzene (VII). The key intermediate 1,2,4-trihydroxybenzene is ring cleaved by intracellular cell extracts to produce, after reduction, beta-ketoadipic acid. In this pathway, initial reduction of a nitroaromatic group generates the peroxidase substrate II. Oxidation of II releases methanol and generates 4-nitro-1,2-benzoquinone (XII), which is recycled by reduction and methylation reactions to regenerate intermediates which are in turn substrates for peroxidase-catalyzed oxidation leading to removal of the second nitro group. Thus, this unique pathway apparently results in the removal of both aromatic nitro groups before ring cleavage takes place.
PMCID: PMC195195  PMID: 1539977
14.  ANTI-RHEUMATIC FORMULATIONS FROM AYURVEDA 
Ancient Science of Life  1991;11(1-2):66-69.
Rheumatic disorders like Amavata, Sandhivata and Vatarakta are elaborately described in ayurvedic literature. Preliminary survey of literature shows that about 247 formulations are recommended for these rheumatic disorders. These formulations generally include guggulu compounds, compounds of plant powders, decoctions, medicated ghees, oils, electuaries etc. Therapeutic potential of ayurvedic concepts and a brief review of Ayurvedic formulations are also discussed.
PMCID: PMC3336571  PMID: 22556565
15.  CRITICAL STUDY OF THE ASAVAISHTA PREPARATIONS OF BRHATIRAYEE 
Ancient Science of Life  1990;9(3):125-133.
This study deals with Asavarishta preparations of the Ayurvedic System of medicine and scans various classical texts to find out the different types of constituents required for their preparation along with their proportions, the method of preparation, the time required to complete the process, the fermentations pots, the fermenting materials, the place and time (season) of fermentation etc. with a view to develop certain common norms for their preparation.
PMCID: PMC3331328  PMID: 22557687
16.  AN EXPERIMENTAL STUDY OF KUTAJARISHTA WITH SPECIAL REFERENCE TO AMOEBIASIS 
Ancient Science of Life  1988;8(2):100-102.
A study on Kutajarista’ was carried out and it was tested for anti-amoebic action which is presented in this paper.
PMCID: PMC3331359  PMID: 22557637
17.  EXPERIMENTAL MODEL FOR THE STUDY OF ANTIDIABETIC EFFECT OF AYURVEDIC METALLIC PREPARATIONS 
Ancient Science of Life  1987;7(1):49-54.
To Substantiate the claims of Ayurvedic classics, animal experimentation with modern technology has been carried out in this paper. Various methods have been described for induction diabetes Mellitus or any other disease. Now it is the duty of an Ayurvedist to select the suitable method on the guidelines of Ayurvedic aetiology to produce a condition stimulating to Prameha (Madhumeha) and the best suitable method (Folin-Wu) for estimating the sugar content of blood is adopted. Besides this one must do experiment in all species and should confirm the finding by clinical evaluation. Side by side toxicity studies are also necessary.
PMCID: PMC3331374  PMID: 22557588
18.  SVARNA – VANGA – A SHORT DURATION TOXICITY STUDY 
Ancient Science of Life  1985;5(2):86-90.
Swarna – Vanga, an Ayurvedic preparation, is used in the treatment mainly of Pramehas (genitor urinary and metabolic disorders), Sveta Pradara (Leucorrhoea), Kasa – Swasa (Respiratory disorders), etc. The drug contains tin and sulphur as major components along with traces of mercury, iron and aluminum. According to modern point of view certain metals have been claimed toxic to both human and animal. Since Svarna – Vanga contains these metals, it is essential to screen out its toxic effect, if any, although it is claimed in Ayurveda that when a metal is processed as prescribed, it become non – toxic or the least toxic. Considering the above facts, an animal experiment was carried out for short duration (14 days) to screen the toxic effects of Svarna – Vanga (SV) in increasing doses of the drug starting from the maximum therapeutic dose (12.5 mg / 100 gm b.wt / day). The drug was found to have no toxic effects in tissues of the animal at doses of 12.5 mg and 25 mg / 100 gm b.wt. / day. Fine fatty vacuolization in liver and focal superficial mucosal degeneration and necrosis of small intestine confined to one animal each at dose of 50 mg / 100gm b.wt. and 100 mg/ 100 gm. b.wt. / day were observed. Our study indicates that the drug has no toxic effect on tissues at therapeutic dose.
PMCID: PMC3331445  PMID: 22557505
19.  STUDY ON THE VRSYA PROPERTY (TESTICULAR REGENERATIVE POTENTIAL) OF VANGA BHASMA 
Ancient Science of Life  1985;5(1):42-48.
Vanga bhasma, a popular Ayurvedic Medicine is prepared from Tin metal. This drug is claimed to have ‘Sukrala’ (Semenaugmentator), ‘vrsya’ (good for testis) properties in the literature and is widely in vogue for genito – urinary disorders in Ayurvedic practice. An experimental evaluation of the claim has been undertaken and the results have been presented in this paper. The drug in reference is found to have testicular regenerative potential on cadmium induced testicular degeneration in albino tats, when administered orally. This effect on ‘Sukravaha Srotomula’ (testis) appears to corroborate “Sukrala” and “Vrsya” properties attributed to the drug in Ayuredic literature.
PMCID: PMC3331434  PMID: 22557499
Vanga bhasma; Sukrala; Vrsyam Sukravaha Srotas; Cadmium induced partial testicular degeneration; zinc sulphate
20.  ROLE OF MERCURY IN SVARNA VANGA PREPARATION 
Ancient Science of Life  1985;4(4):202-204.
Svarna Vanga, an important Ayurvedic tin preparation having mercury as one of its ingredients, is mainly indicated in the treatment of Pramehas (genitourinary disorders). What role does mercury play in its preparation is not known. Hence present study has been planned with a view to prepare SvarnaVanga using mercury in different amounts. It was observed that the amount of mercury atleast in half proportion to in tin Kajjali, is considered necessary in making the standard Svarna-Vanga preparation.
PMCID: PMC3331525  PMID: 22557478
21.  TOXICITY STUDIES ON VANGA BHASMA (Part I – with special reference to G. I. T. Liver and Pancreas) 
Ancient Science of Life  1984;4(1):32-35.
Vangabhasma is a popular and effective dosage form prepared from tin metal in Ayurvedic practice. Since modern literature attributes certain toxicity to tin salts, an attempt is made to screen the acute and sub-acute toxicity of Vangabhasma in the form, dose and route as is in the practice or Ayurveda. In this paper, dose-effect relation of Vangabhasma on Digestive system (G.I. T., Liver and Pancreas) has been presented. But for local irritation, no significant toxicity attributable to Vangabhasma has been observed even in eight times higher dose than therapeutic dose, on exposure to the drug for ten days.
PMCID: PMC3331483  PMID: 22557446
23.  No Apology for Geriatrics 
British Medical Journal  1973;4(5887):277-280.
A geriatric department is described where turnover has more than kept pace with demand over a period of 17 years. The department provides two basic services—a hospital service to the pensionable population in the community, and support to other hospital departments that care for the elderly.
Community emphasis is on a high turnover of patients, enabling early contract and treatment. Over the years a fall in the proportion of “chronic” to “acute” beds has occurred and this has been achieved by having the majority of beds in the general hospital, where it is possible to provide a comprehensive medical service. The hospital role has been to prevent overloading acute resources with potential long-stay cases, and this has been possible without compromising our community obligations.
PMCID: PMC1587359  PMID: 4753243

Results 1-24 (24)