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Anatomy & Cell Biology (1)
Asian Journal of Andrology (1)
Bju International (1)
Journal of Assisted Reproduction and Genetics (1)
Takenaka, Atsushi (4)
Chancellor, Michael B. (1)
Georgiou, Ioannis (1)
Georgiou, Ioannis A. (1)
Hatzi, Elissavet (1)
Hatzi, Elissavet G. (1)
Honda, Masashi (1)
Inoue, Seiya (1)
Kaponis, Apostolos (1)
Kaponis, Apostolos I. (1)
Kosmas, Ioannis P. (1)
Lazaros, Leandros (1)
Lazaros, Leandros A. (1)
Makrydimas, Georgios (1)
Makrydimas, Georgios V. (1)
Masumoto, Hiroshi (1)
Matsubara, Akio (1)
Murakami, Gen (1)
Rodríguez-Vázquez, Jose Francisco (1)
Sofikitis, Nikolaos (1)
Sofikitis, Nikolaos V. (1)
Stefos, Theodoros (1)
Stefos, Theodoros I. (1)
Xita, Nectaria (1)
Xita, Nectaria V. (1)
Yoshimura, Naoki (1)
Zikopoulos, Konstantinos (1)
Zikopoulos, Konstantinos A. (1)
Year of Publication
Sensory Neuron-Specific Receptor-Mediated Regulation of Micturition Reflex in Urethane-Anesthetized Rats
Chancellor, Michael B.
To investigate the effect of sensory neuron-specific receptors (SNSRs) activation on the micturition reflex in rats.
MATERIALS AND METHODS
Continuous cystometrograms (CMG, 0.04ml/min) were performed in female Sprague-Dawley rats under urethane anesthesia. After stable micturition cycles were established, a selective rat SNSR1 agonist, bovine adrenal medulla 8–22 (BAM8-22), was administered intravenously or intrathecally in normal rats or rats pretreated with capsaicin 4 days before the experiments. Micturition parameters were recorded and compared before and after drug administration.
Intravenous administration of BAM8-22 (3 to 100 μg/kg) significantly increased intercontraction intervals in dose dependent fashion, but did not affect residual urine or baseline pressure at any doses tested. Intrathecal administration of BAM8-22 (0.01 to 0.3 μg) also increased intercontraction intervals in dose dependent fashion, but did not affect residual urine or baseline pressure at any doses tested. These inhibitory effects of intravenous (30 μg/kg) or intrathecal (0.3 μg) administration of BAM8-22 were still observed after capsaicin pretreatment.
These results indicate that in urethane-anesthetized rats activation of SNSRs can inhibit the micturition reflex via the pathways independent of capsaicin sensitive C-fibers. Thus SNSRs could be a potential target for the treatment of bladder dysfunction such as overactive bladder.
bladder; sensory-neuron-specific receptors; capsaicin; spinal cord; rats
Aromatase (CYP19) gene variants influence ovarian response to standard gonadotrophin stimulation
Lazaros, Leandros A.
Hatzi, Elissavet G.
Xita, Nectaria V.
Makrydimas, Georgios V.
Kaponis, Apostolos I.
Kosmas, Ioannis P.
Sofikitis, Nikolaos V.
Stefos, Theodoros I.
Zikopoulos, Konstantinos A.
Georgiou, Ioannis A.
Journal of Assisted Reproduction and Genetics
The association of cytochrome P450 aromatase gene CYP19(TTTA)n polymorphism with ovarian response to FSH stimulation was explored.
Three hundred women undergoing medically assisted reproduction and 300 women with at least one spontaneous pregnancy participated in the study. CYP19(TTTA)n polymorphism was genotyped, while serum hormones were determined. During oocyte retrieval, the follicular size, the follicle and oocyte numbers were recorded.
Six CYP19(TTTA)n alleles with 7 to 12 repeats were revealed. Women homozygous for long CYP19(TTTA)n alleles presented with lower serum FSH levels at the third day of the menstrual cycle (p < 0.001) and higher large follicle numbers (p < 0.01), compared to women homozygous for short CYP19(TTTA)n alleles. The CYP19(TTTA)7 allele was associated with higher serum FSH levels (p < 0.003), with lower total follicle (p < 0.02) and large follicle numbers (p < 0.03), while CYP19(TTTA)7 allele-carriers presented more frequently with small follicles than CYP19(TTTA)7 allele-non carriers (p < 0.01).
CYP19 genetic variants were associated with ovarian reserve and response to standard gonadotrophin stimulation of women undergoing in vitro fertilization.
Controlled ovarian stimulation; CYP19; IVF; Ovarian response; Tubal infertility
Phosphatidylethanolamine N-methyltransferase and choline dehydrogenase gene polymorphisms are associated with human sperm concentration
Asian Journal of Andrology
Choline is a crucial factor in the regulation of sperm membrane structure and fluidity, and this nutrient plays an important role in the maturation and fertilizing capacity of spermatozoa. Transcripts of phosphatidylethanolamine N-methyltransferase (PEMT) and choline dehydrogenase (CHDH), two basic enzymes of choline metabolism, have been observed in the human testis, demonstrating their gene expression in this tissue. In the present study, we explored the contribution of the PEMT and CHDH gene variants to sperm parameters. Two hundred oligospermic and 250 normozoospermic men were recruited. DNA was extracted from the spermatozoa, and the PEMT −774G>C and CHDH +432G>T polymorphisms were genotyped. The genotype distribution of the PEMT −774G>C polymorphism did not differ between oligospermic and normozoospermic men. In contrast, in the case of the CHDH +432G>T polymorphism, oligospermic men presented the CHDH 432G/G genotype more frequently than normozoospermic men (62% vs. 42%, P<0.001). The PEMT 774G/G genotype was associated with a higher sperm concentration compared to the PEMT 774G/C and 774C/C genotypes in oligospermic men (12.5±5.6×106 spermatozoa ml−1 vs. 8.3±5.2×106 spermatozoa ml−1, P<0.002) and normozoospermic men (81.5±55.6×106 vs. 68.1±44.5×106 spermatozoa ml−1, P<0.006). In addition, the CHDH 432G/G genotype was associated with higher sperm concentration compared to CHDH 432G/T and 432T/T genotypes in oligospermic (11.8±5.1×106 vs. 7.8±5.3×106 spermatozoa ml−1, P<0.003) and normozoospermic men (98.6±62.2×106 vs. 58.8±33.6×106 spermatozoa ml−1, P<0.001). In our series, the PEMT −774G>C and CHDH +432G>T polymorphisms were associated with sperm concentration. This finding suggests a possible influence of these genes on sperm quality.
choline dehydrogenase; male infertility; phosphatidylethanolamine N-methyltransferase; phospholipids; sperm concentration
Reappraisal of intergender differences in the urethral striated sphincter explains why a completely circular arrangement is difficult in females: a histological study using human fetuses
Rodríguez-Vázquez, Jose Francisco
Anatomy & Cell Biology
To investigate why the development of a completely circular striated sphincter is so rare, we examined histological sections of 11 female and 11 male mid-term human fetuses. In male fetuses, the striated muscle initially extended in the frontal, rather than in the horizontal plane. However, a knee-like portion was absent in the female fetal urethra because, on the inferior side of the vaginal end, a wide groove for the future vestibule opened inferiorly. Accordingly, it was difficult for the developing striated muscle to surround the groove, even though there was not a great difference in width or thickness between the female vestibule and the male urethra. The development of a completely circular striated sphincter seems to be impossible in females because of interruption of the frontal plane by the groove-like vestibule. However, we cannot rule out the possibility that before descent of the vagina, the urethral striated muscle extends posteriorly.
Urethal rhabdosphincter; Genital tract; Urogenital sinus; Colliculus; Human fetus
Results 1-4 (4)
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