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Advances in Dental Research (1)
BioMed Research International (1)
Abrams, William R. (1)
Chen, Zongyuan (1)
Corstjens, Paul L. A. M. (1)
Geva, Eran (1)
Giannobile, W.V. (1)
González, Jesús M. (1)
Malamud, D. (1)
Malamud, Daniel (1)
McDevitt, J.T. (1)
Niedbala, R. Sam (1)
Niedbala, R.S. (1)
Tanke, Hans J. (1)
Wong, D.T.W. (1)
Zhou, Peng (1)
de Dood, Claudia J. (1)
Year of Publication
Translational and Clinical Applications of Salivary Diagnostics
Advances in Dental Research
There have been significant advances in techniques for the detection of biomarker signals in the oral cavity (e.g., ELISAs for proteins, PCR for RNA and DNA) as well as the engineering and development of microfluidic approaches to make oral-based point-of-care (POC) methods for the diagnosis for both local and systemic conditions a reality. In this section, we focus on three such approaches, namely, periodontal disease management, early markers for systemic diseases, and salivary markers useful for pharmacogenomic studies. Novel approaches using non-invasive, salivary samples and user-friendly devices offer results that are as sensitive and specific as laboratory-based analyses using blood or urine.
biomarkers; saliva; cardiovascular disease; pharmacogenomics; periodontal diseases; lab-on-a-chip
Development of a Generic Microfluidic Device for Simultaneous Detection of Antibodies and Nucleic Acids in Oral Fluids
Abrams, William R.
de Dood, Claudia J.
González, Jesús M.
Tanke, Hans J.
Corstjens, Paul L. A. M.
BioMed Research International
A prototype dual-path microfluidic device (Rheonix CARD) capable of performing simultaneously screening (antigen or antibody) and confirmatory (nucleic acid) detection of pathogens is described. The device fully integrates sample processing, antigen or antibody detection, and nucleic acid amplification and detection, demonstrating rapid and inexpensive “sample-to-result” diagnosis with performance comparable to benchtop analysis. For the chip design, a modular approach was followed allowing the optimization of individual steps in the sample processing process. This modular design provides great versatility accommodating different disease targets independently of the production method. In the detection module, a lateral flow (LF) protocol utilizing upconverting phosphor (UCP) reporters was employed. The nucleic acid (NA) module incorporates a generic microtube containing dry reagents. Lateral flow strips and PCR primers determine the target or disease that is diagnosed. Diagnosis of HIV infection was used as a model to investigate the simultaneous detection of both human antibodies against the virus and viral RNA. The serological result is available in less than 30 min, and the confirmation by RNA amplification takes another 60 min. This approach combines a core serological portable diagnostic with a nucleic acid-based confirmatory test.
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