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Acta Crystallographica Section B: Structural Science (1)
Nishi, Koji (2)
Fujiyama, Shinjiro (1)
Fukai, Toshio (1)
Ikeda, Toshihiko (1)
Iwama, Wataru (1)
Iwase, Yumiko (1)
Kamiya, Natsumi (1)
Komatsu, Hajime (1)
Kudo, Tomokazu (1)
Momose, Yasunori (1)
Nasuno, Tomomi (1)
Okudaira, Kazuho (1)
Onodera, Kenji (1)
Takeda, Kazuyoshi (1)
Umemura, Shin-ichiro (1)
Yokomori, Yoshinobu (1)
Yumita, Nagahiko (1)
Year of Publication
Involvement of Reactive Oxygen Species in Sonodynamically Induced Apoptosis Using a Novel Porphyrin Derivative
In this study, we investigated the induction of apoptosis by ultrasound in the presence of the novel porphyrin derivative DCPH-P-Na(I). HL-60 cells were exposed to ultrasound for up to 3 min in the presence and absence of DCPH-P-Na(I), and the induction of apoptosis was examined by analyzing cell morphology, DNA fragmentation, and caspase-3 activity. Reactive oxygen species were measured by means of ESR and spin trapping technique. Cells treated with 8 μM DCPH-P-Na(I) and ultrasound clearly showed membrane blebbing and cell shrinkage, whereas significant morphologic changes were not observed in cells exposed to either ultrasound or DCPH-P-Na(I) alone. Also, DNA ladder formation and caspase-3 activation were observed in cells treated with both ultrasound and DCPH-P-Na(I) but not in cells treated with ultrasound or DCPH-P-Na(I) alone. In addition, the combination of DCPH-P-Na(I) and the same acoustical arrangement of ultrasound substantially enhanced nitroxide generation by the cells. Sonodynamically induced apoptosis, caspase-3 activation, and nitroxide generation were significantly suppressed by histidine. These results indicate that the combination of ultrasound and DCPH-P-Na(I) induced apoptosis in HL-60 cells. The significant reduction in sonodynamically induced apoptosis, nitroxide generation, and caspase-3 activation by histidine suggests active species such as singlet oxygen are important in the sonodynamic induction of apoptosis. These experimental results support the possibility of sonodynamic treatment for cancer using the induction of apoptosis.
Apoptosis; Sonodynamic therapy; Ultrasound; DCPH-P-Na(I); HL-60 cells, Reactive Oxygen, Caspase-3.
Determining the structure of a benzene7.2-silicalite-1 zeolite using a single-crystal X-ray method
Acta Crystallographica Section B: Structural Science
An orthorhombic benzene-silicalite-1 single crystal was obtained from a monoclinic twin crystal, and the structure was determined by a single-crystal method for the first time.
A simple method for preparing orthorhombic single crystals of benzene-silicalite-1 was developed. A silicalite-1 crystal was pressed with a weight of 2 g along the +c and −c crystallographic axes while the temperature was increased to 473 K. The temperature was then slowly reduced to 313 K, and these heating and cooling steps were repeated three times. After the orthorhombic single crystals adsorbed benzene, the crystal structure of the resulting benzene-silicalite-1 was determined. There were two kinds of benzene molecules in the asymmetric unit. One was located at the intersection of the straight channels and the sinusoidal channels with the benzene ring parallel to the ac plane. The other benzene was located in the middle of the straight channel.
ZSM-5; MFI; silicalite-1; benzene-silicalite-1
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