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1.  A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay 
AMB Express  2012;2:51.
A method for the detection of haloalkane conversion to the corresponding alcohols by haloalkane dehalogenases is described. It is based on a multistage enzyme reaction which allows for the analysis of alkyl halides in buffered systems. Irreversible hydrolytic dehalogenation catalyzed by haloalkane dehalogenase DhaA from Rhodococcus erythropolis transfers an alkyl halide into a corresponding alcohol that is further oxidized by alcohol oxidase AOX from Pichia pastoris yielding a respective aldehyde and hydrogen peroxide easily detectable via the horseradish peroxidase catalyzed oxidation of chromogenic molecules. Due to its high sensitivity (0.025 mM, 0.43 ppm for 1,3-dibromopropane), low expenditure and the ability of handling a large number of samples in parallel, this method is an attractive alternative to existing procedures for the monitoring of both haloalkanes and dehalogenases.
doi:10.1186/2191-0855-2-51
PMCID: PMC3487978  PMID: 23006907
Alcohol oxidase; Haloalkane dehalogenase; Haloalkanes; Horseradish peroxidase; Multistage enzyme reaction

Results 1-1 (1)