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1.  Defective cranial skeletal development, larval lethality and haploinsufficiency in Myod mutant zebrafish 
Developmental biology  2011;358(1):102-112.
Summary
Myogenic regulatory factors of the myod family (MRFs) are transcription factors essential for mammalian skeletal myogenesis. Here we show that a mutation in the zebrafish myod gene delays and reduces early somitic and pectoral fin myogenesis, reduces miR-206 expression, and leads to a persistent reduction in somite size until at least the independent feeding stage. A mutation in myog, encoding a second MRF, has little obvious phenotype at early stages, but exacerbates the loss of somitic muscle caused by lack of Myod. Mutation of both myod and myf5 ablates all skeletal muscle. Haploinsufficiency of myod leads to reduced embryonic somite muscle bulk. Lack of Myod causes a severe reduction in cranial musculature, ablating most muscles including the protractor pectoralis, a putative cucullaris homologue. This phenotype is accompanied by a severe dysmorphology of the cartilaginous skeleton and failure of maturation of several cranial bones, including the opercle. As myod expression is restricted to myogenic cells, the data show that myogenesis is essential for proper skeletogenesis in the head.
doi:10.1016/j.ydbio.2011.07.015
PMCID: PMC3360969  PMID: 21798255
muscle; zebrafish; myosin; slow; fiber; fast; myod; myogenin; myf5; miR-206; skeleton; bone; cartilage; head; fin; haploinsufficiency
2.  Reverse genetics in zebrafish by TILLING 
TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Drosophila, Caenorhabditis elegans, rat, medaka and zebrafish and for the discovery of naturally occurring polymorphisms in humans. This review summarizes current TILLING methodologies as they have been applied to the zebrafish, ongoing TILLING projects and resources in the zebrafish community, and the future of zebrafish TILLING.
doi:10.1093/bfgp/eln046
PMCID: PMC2899843  PMID: 19028802
zebrafish; TILLING; Cel1 mismatch cleavage; resequencing; reverse genetics

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