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author:("Goyal, kapila")
1.  Neurocysticercosis: A disease of neglect 
Tropical Parasitology  2013;3(2):106-113.
Neurocysticercosis (NCC) is a neglected tropical disease caused by larval forms of the parasite Taenia solium lodging in central nervous system (CNS). There is a huge morbidity and debilitation due to CNS manifestations of NCC in developing and underdeveloped regions of the globe, mainly Asian, African and Latin American countries. It is the cause of epilepsy in about 1% of the population of endemic countries and is the underlying etiology in about 15-50% persons with epilepsy, depending upon the geographical region. There is no perfect diagnostic method and the diagnosis relies on a combination of clinical, radio-imaging, immunologic and epidemiologic data. Treatment includes anti-parasitic treatment by cysticidal drugs and management of associated symptoms and complications. The disease is eradicable and control depends on an integrated and coordinated involvement of international bodies like the World Health Organization along with scientific institutions and political and administrative strata of the endemic countries to provide the essential tools such as adequate sanitation, live-stock management, health education and improved socio-economic conditions.
PMCID: PMC3889086  PMID: 24470993
Neglected tropical disease; neurocysticercosis; Taenia solium
2.  Multiplex PCR for Rapid Diagnosis of Gastrointestinal Tuberculosis 
Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance.
To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB.
Materials and Methods:
MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 Non GITB (control group) patients.
MPB64 PCR had sensitivity and specificity of 90% and 100% for confirmed GITB cases. In 29 clinically diagnosed but unconfirmed GITB cases, MPCR was positive in 72.41%. MPCR was negative in all control group patients. The overall sensitivity and specificity of microscopy, culture, histopathology and MPCR was 5%, 2% 20% and 77.5% and 100%, 100%, 100% and 100% respectively.
MPCR has good sensitivity and specificity in diagnosing gastrointestinal tuberculosis.
PMCID: PMC3703210  PMID: 23853431
Gastrointestinal tract; Multiplex PCR; Tuberculosis
3.  Trichomoniasis and Lactoferrin: Future Prospects 
Trichomonas vaginalis is a parasitic protozoan which infects the urogenital tract and requires iron as an essential nutrient. Iron is known to upregulate various adhesins required for cytoadherance and other factors involved in pathogenesis. At mucosal surfaces, iron is chelated by lactoferrin resulting in low levels of free iron. However, pathogens have evolved mechanisms for an increased uptake of iron. The present review highlights the role of iron in survival of Trichomonas during fluctuating concentrations of iron at mucosal surfaces during the menstrual cycle. Future prospects in terms of new drug and vaccine targets related to iron and its receptors have also been described.
PMCID: PMC3439953  PMID: 22988421
4.  Evaluation of dried blood spots collected on filter paper for serodiagnosis of human hydatidosis by enzyme-linked immunosorbent assay 
Tropical Parasitology  2012;2(2):119-123.
Background and Objectives:
Serological diagnosis of hydatidosis is usually performed by detecting the circulating antibodies in serum by ELISA. The present study was carried out to standardize and evaluate procedure of the ELISA using elute from dried blood spots (DBS) on filter paper and blood stored at different temperatures and at different durations for its further application under field conditions.
Materials and Methods:
Dried blood spots were collected from fifty study subjects and fifty control subjects and evaluated for the detection of IgG antibodies against hydatid. Samples were stored at room temperature and 4°C and tested by ELISA at 0, 15 and 30 days.
The present study shows that elute of DBS on filter paper can be stored at room temperature for a maximum of 30 days without a decrease in antibody titer as compared to serum samples tested by ELISA.
The collection of blood sample on filter paper may serve useful purpose in resource limited countries for carrying out sero-epidemiological surveys at a cost effective level.
PMCID: PMC3680872  PMID: 23767019
Dried blood spots; enzyme-linked immunosorbent assay; hydatidosis
5.  Nonstructural Protein NS1: Giving a New Structure to Dengue Diagnosis 
Journal of Clinical Microbiology  2010;48(12):4688-4689.
PMCID: PMC3008478  PMID: 21115848

Results 1-5 (5)