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1.  Fragile X protein mitigates TDP-43 toxicity by remodeling RNA granules and restoring translation 
Human Molecular Genetics  2015;24(24):6886-6898.
RNA dysregulation is a newly recognized disease mechanism in amyotrophic lateral sclerosis (ALS). Here we identify Drosophila fragile X mental retardation protein (dFMRP) as a robust genetic modifier of TDP-43-dependent toxicity in a Drosophila model of ALS. We find that dFMRP overexpression (dFMRP OE) mitigates TDP-43 dependent locomotor defects and reduced lifespan in Drosophila. TDP-43 and FMRP form a complex in flies and human cells. In motor neurons, TDP-43 expression increases the association of dFMRP with stress granules and colocalizes with polyA binding protein in a variant-dependent manner. Furthermore, dFMRP dosage modulates TDP-43 solubility and molecular mobility with overexpression of dFMRP resulting in a significant reduction of TDP-43 in the aggregate fraction. Polysome fractionation experiments indicate that dFMRP OE also relieves the translation inhibition of futsch mRNA, a TDP-43 target mRNA, which regulates neuromuscular synapse architecture. Restoration of futsch translation by dFMRP OE mitigates Futsch-dependent morphological phenotypes at the neuromuscular junction including synaptic size and presence of satellite boutons. Our data suggest a model whereby dFMRP is neuroprotective by remodeling TDP-43 containing RNA granules, reducing aggregation and restoring the translation of specific mRNAs in motor neurons.
doi:10.1093/hmg/ddv389
PMCID: PMC5007633  PMID: 26385636
2.  HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus 
Journal of Virology  2016;90(11):5353-5367.
ABSTRACT
Epstein-Barr virus (EBV) establishes latent infections as multicopy episomes with complex patterns of viral gene transcription and chromatin structure. The EBV origin of plasmid replication (OriP) has been implicated as a critical control element for viral transcription, as well as viral DNA replication and episome maintenance. Here, we examine cellular factors that bind OriP and regulate histone modification, transcription regulation, and episome maintenance. We found that OriP is enriched for histone H3 lysine 4 (H3K4) methylation in multiple cell types and latency types. Host cell factor 1 (HCF1), a component of the mixed-lineage leukemia (MLL) histone methyltransferase complex, and transcription factor OCT2 (octamer-binding transcription factor 2) bound cooperatively with EBNA1 (Epstein-Barr virus nuclear antigen 1) at OriP. Depletion of OCT2 or HCF1 deregulated latency transcription and histone modifications at OriP, as well as the OriP-regulated latency type-dependent C promoter (Cp) and Q promoter (Qp). HCF1 depletion led to a loss of histone H3K4me3 (trimethylation of histone H3 at lysine 4) and H3 acetylation at Cp in type III latency and Qp in type I latency, as well as an increase in heterochromatic H3K9me3 at these sites. HCF1 depletion resulted in the loss of EBV episomes from Burkitt's lymphoma cells with type I latency and reactivation from lymphoblastoid cells (LCLs) with type III latency. These findings indicate that HCF1 and OCT2 function at OriP to regulate viral transcription, histone modifications, and episome maintenance. As HCF1 is best known for its function in herpes simplex virus 1 (HSV-1) immediate early gene transcription, our findings suggest that EBV latency transcription shares unexpected features with HSV gene regulation.
IMPORTANCE EBV latency is associated with several human cancers. Viral latent cycle gene expression is regulated by the epigenetic control of the OriP enhancer region. Here, we show that cellular factors OCT2 and HCF1 bind OriP in association with EBNA1 to maintain elevated histone H3K4me3 and transcriptional enhancer function. HCF1 is known as a transcriptional coactivator of herpes simplex virus (HSV) immediate early (IE) transcription, suggesting that OriP enhancer shares aspects of HSV IE transcription control.
doi:10.1128/JVI.00239-16
PMCID: PMC4934754  PMID: 27009953
3.  Proceedings of the 7th Biannual International Symposium on Nasopharyngeal Carcinoma 2015 
Tan, IB | Chang, Ellen T. | Chen, Chien-Jen | Hsu, Wan-Lun | Chien, Yin-Chu | Hildesheim, Allan | McKay, James D. | Gaborieau, Valerie | Kaderi, Mohamed Arifin Bin | Purnomosari, Dewajani | Voegele, Catherine | LeCalvez-Kelm, Florence | Byrnes, Graham | Brennan, Paul | Devi, Beena | Li, L. | Zhang, Y. | Fan, Y. | Sun, K. | Du, Z. | Sun, H. | Chan, A. T. | Tsao, S. W. | Zeng, Y. X. | Tao, Q. | Busson, Pierre | Lhuillier, Claire | Morales, Olivier | Mrizak, Dhafer | Gelin, Aurore | Kapetanakis, Nikiforos | Delhem, Nadira | Mansouri, Sheila | Cao, Jennifer | Vaidya, Anup | Frappier, Lori | Wai, Lo Kwok | Chen, Sui-Hong | Du, Jin-lin | Ji, Ming-Fang | Huang, Qi-Hong | Liu, Qing | Cao, Su-Mei | Doolan, Denise L. | Coghill, Anna | Mulvenna, Jason | Proietti, Carla | Lekieffre, Lea | Bethony, Jeffrey | Hildesheim, and Allan | Fles, Renske | Indrasari, Sagung Rai | Herdini, Camelia | Martini, Santi | Isfandiari, Atoillah | Rhomdoni, Achmad | Adham, Marlinda | Mayangsari, Ika | van Werkhoven, Erik | Wildeman, Maarten | Hariwiyanto, Bambang | Hermani, Bambang | Kentjono, Widodo Ario | Haryana, Sofia Mubarika | Schmidt, Marjanka | Tan, IB | O’Sullivan, Brian | Ozyar, Enis | Lee, Anne W. M. | Zeng, Mu-Sheng | Gao, Xiaojiang | Tang, Minzhong | Martin, Pat | Zeng, Yi | Carrington, Mary | Coghill, Anna E. | Bu, Wei | Nguyen, Hanh | Hsu, Wan-Lun | Yu, Kelly J. | Lou, Pei-Jen | Wang, Cheng-Ping | Chen, Chien-Jen | Hildesheim, Allan | Cohen, Jeffrey I. | King, Ann D. | Chien, Yin-Chu | Hsu, Wan-Lun | Yu, Kelly J. | Chen, Tseng-Cheng | Lin, Ching-Yuan | Tsou, Yung-An | Leu, Yi-Shing | Laio, Li-Jen | Chang, Yen-Liang | Wang, Cheng-Ping | Hua, Chun-Hun | Wu, Ming-Shiang | Hsiao, Chu-Hsing Kate | Lee, Jehn-Chuan | Tsai, Ming-Hsui | Cheng, Skye Hung-Chun | Lou, Pei-Jen | Hildesheim, Allan | Chen, Chien-Jen | Hsu, Wan-Lun | Yu, Kelly J. | Chien, Yin-Chu | Chen, Tseng-Cheng | Lin, Ching-Yuan | Tsou, Yung-An | Leu, Yi-Shing | Liao, Li-Jen | Chang, Yen-Liang | Yang, Tsung-Lin | Hua, Chun-Hun | Wu, Ming-Shiang | Hsiao, Chu-Hsing Kate | Lee, Jehn-Chuan | Tsai, Ming-Hsui | Cheng, Skye Hung-Chun | Ko, Jenq-Yuh | Hildesheim, Allan | Chen, Chien-Jen | Ko, Josephine Mun Yee | Dai, Wei | Kwong, Dora | Ng, Wai Tong | Lee, Anne | Ngan, Roger Kai Cheong | Yau, Chun Chung | Tung, Stewart | Lung, Maria Li | Ji, Mingfang | Sheng, Wei | Ng, Mun Hon | Cheng, Weimin | Yu, Xia | Wu, Biaohua | Wei, Kuangrong | Zhan, Jun | Zeng, Yi Xin | Cao, Su Mei | Xia, Ningshao | Yuan, Yong | Cui, Qian | Xu, Miao | Bei, Jin-Xin | Zeng, Yi-Xin | Şahin, B | Dizman, A | Esassolak, M | İkizler, A Saran | Yıldırım, HC | Çaloğlu, M | Atalar, B | Akman, F | Demiroz, C | Atasoy, BM | Canyilmaz, E | Igdem, S | Ugurluer, G | Kütük, T | Akmansoy, M | Ozyar, E | Sommat, Kiattisa | Wang, Fu Qiang | Kwok, Li-Lian | Tan, Terence | Fong, Kam Weng | Soong, Yoke Lim | Cheah, Shie Lee | Wee, Joseph | Casanova, M | Özyar, E | Patte, C | Orbach, D | Ferrari, A | Cristine, VF | Errihani, H | Pan, J | Zhang, L | Liji, S | Grzegorzewski, K | Gore, L | Varan, A | Hutajulu, Susanna Hilda | Khuzairi, Guntara | Herdini, Camelia | Kusumo, Henry | Hardianti, Mardiah Suci | Taroeno-Hariadi, Kartika Widayati | Purwanto, Ibnu | Kurnianda, Johan | Messick, Troy E. | Malecka, Kimberly | Tolvinski, Lois | Soldan, Samantha | Deakyne, Julianna | Song, Hui | van den Heuvel, Antonio | Gu, Baiwei | Cassel, Joel | McDonnell, Mark | Smith, Garry R. | Velvadapu, Venkata | Bian, Haiyan | Zhang, Yan | Carlsen, Marianne | Chen, Shuai | Donald, Alastair | Lemmen, Christian | Reitz, Allen B. | Lieberman, Paul M. | Chan, King Chi | Chan, Lai Sheung | Lo, Kwok Wai | Yip, Timothy Tak Chun | Ngan, Roger Kai Cheong | Kahn, Michael | Lung, Maria Li | Mak, Nai Ki | Liu, Fei-Fei | Khaali, Wafa | Thariat, Juliette | Fantin, Laurence | Spirito, Flavia | Khyatti, Meriem | Driss, El Khalil Ben | Olivero, Sylvain | Maryanski, Janet | Doglio, Alain | Xia, Mengxue | Xia, Yunfei | Chang, Hui | Shaw, Rachel | Rahaju, Pudji | Hardianti, Mardiah Suci | Wisesa, Sindhu | Taroeno-Harijadi, Kartika Widayati | Purwanto, Ibnu | Hariwiyanto, Bambang | Dhamiyati, Wigati | Kurnianda, Johan | Tan, Sang-Nee | Sim, Sai-Peng | Yusuf, Muhtarum | Romdhoni, Ahmad C. | K, Widodo Ario | Rantam, Fedik Abdul | Sugiyanto | Aryati, Lina | Adi-Kusumo, Fajar | Hardianti, Mardiah Suci | Bintoro, SY | Oktriani, R. | Herawati, C. | Surono, A. | Haryana, Sofia M. | Zhong, L. | Li, L. | Ma, B. B. | Chan, A. T. | Tao, Q. | Kalra, M. | Ngo, M. | Perna, S. | Leen, A. | Lapteva, N. | Rooney, C. M. | Gottschalk, S. | Mustikaningtyas, Elida | Herawati, Sri | Romdhoni, Achmad C. | Ji, Mingfang | Xu, Yarui | Cheng, Weimin | Ge, Shengxiang | Li, Fugui | Ng, M. H. | Tan, Louise SY | Wong, Benjamin | Lim, C. M. | Romdhoni, Achmad C. | Rantam, Fedik A. | Kentjono, Widodo Ario | Madani, Deasy Z. | Akbar, Nur | Permana, Agung Dinasti | Herdini, Camelia | Indrasari, Sagung Rai | Fachiroh, Jajah | Hartati, Dwi | Rahayudjati, T. Baning | Darwis, Iswandi | Hutajulu, Susanna Hilda | Hariwiyanto, Bambang | Dhamiyati, Wigati | Purwanto, Ibnu | Taroeno-Hariadi, Kartika Widayati | Kurnianda, Johan | Wisesa, Sindhu | Hardianti, Mardiah Suci | Hutajulu, Susanna Hilda | Taroeno-Harijadi, Kartika Widayati | Purwanto, Ibnu | Herdini, Camelia | Dhamiyati, Wigati | Kurnianda, Johan | Anwar, Khoirul | Hutajulu, Susanna Hilda | Indrasari, Sagung Rai | Dwidanarti, Sri Retna | Purwanto, Ibnu | Taroeno-Hariadi, Kartika Widayati | Kurnianda, Johan | Pramana, Dominicus Wendhy | Hutajulu, Susanna Hilda | Hariwiyanto, Bambang | Dhamiyati, Wigati | Purwanto, Ibnu | Taroeno-Hariadi, Kartika Widayati | Kurnianda, Johan | Safitri, Diah Ari | Hutajulu, Susanna Hilda | Herdini, Camelia | Danarti, Sri Retna Dwi | Purwanto, Ibnu | Taroeno-Hariadi, Kartika Widayati | Kurnianda, Johan | Taroeno, Suryo A | Wisesa, Sindhu | Taroeno-Hariadi, Kartika Widayati | Purwanto, Ibnu | Hariwiyanto, Bambang | Dhamiyati, Wigati | Kurnianda, Johan | Wijaya, I. | Oehadian, A. | Prasetya, D. | Hsu, Wan-Lun | Chien, Yin-Chu | Yu, Kelly J | Wang, Cheng-Ping | Lin, Ching-Yuan | Tsou, Yung-An | Leu, Yi-Shing | Liao, Li-Jen | Chang, Yen-Liang | Ko, Jenq-Yuh | Hua, Chun-Hun | Wu, Ming-Shiang | Hsiao, Chu-Hsing Kate | Lee, Jehn-Chuan | Tsai, Ming-Hsui | Cheng, Skye Hung-Chun | Lou, Pei-Jen | Hildesheim, Allan | Chen, Chien-Jen | Rahman, Sukri | Budiman, Bestari J. | Novialdi | Rahmadona | Lestari, Dewi Yuri | Yin, C. | Foussadier, A. | Blein, E. | Chen, C. | Ammour, N. Bournet | Khiatti, M. | Cao, S. | Marzaini, Dewi Syafriyetti Soeis | Hartati, Dwi | Rahayujati, Baning | Herdini, Camelia | Fachiroh, Jajah | Gunawan, L. | Mubarika Haryana, S. | Surono, A. | Herawati, C. | Hartono, Michael | Fachiroh, Jajah | Intansari, Umi | Paramita, Dewi Kartikawati | Akbar, Akmal | Fachiroh, Jajah | Paramita, Dewi Kartikawati | Hermawan, Benny | Rahayudjati, T. Baning | Paramita, Dewi K. | Fachiroh, Jajah | Argy, Gabriella | Fachiroh, Jajah | Paramita, Dewi Kartikawati | Hutajulu, Susanna Hilda | Sihotang, Theodora Caroline | Fachiroh, Jajah | Intansari, Umi | Paramita, Dewi Kartikawati | Wahyono, Daniel Joko | Soeharso, Purnomo | Suryandari, Dwi Anita | Lisnawati | Musa, Zanil | Hermani, Bambang | Daker, Maelinda | Tzen, Yeo Jiun | Bakar, Norhasimah | Rahman, Asma’ Saiyidatina Aishah Abdul | Ahmad, Munirah | Chia, Yeo Tiong | Beng, Alan Khoo Soo | Sasikirana, Widyandani | Wardana, Tirta | Radifar, Muhammad | Herawati, Cita | Surono, Agus | Haryana, Sofia Mubarika
BMC Proceedings  2016;10(Suppl 1):1.
Table of contents
A1 Hope and despair in the current treatment of nasopharyngeal cancer
IB Tan
I1 NPC international incidence and risk factors
Ellen T Chang
I2 Familial nasopharyngeal carcinoma and the use of biomarkers
Chien-Jen Chen, Wan-Lun Hsu, Yin-Chu Chien
I3 Genetic susceptibility risk factors for sporadic and familial NPC: recent findings
Allan Hildesheim
I5 Genetic and environmental risk factors for nasopharyngeal cancer in Southeast Asia
James D McKay, Valerie Gaborieau, Mohamed Arifin Bin Kaderi, Dewajani Purnomosari, Catherine Voegele, Florence LeCalvez-Kelm, Graham Byrnes, Paul Brennan, Beena Devi
I6 Characterization of the NPC methylome identifies aberrant epigenetic disruption of key signaling pathways and EBV-induced gene methylation
Li L, Zhang Y, Fan Y, Sun K, Du Z, Sun H, Chan AT, Tsao SW, Zeng YX, Tao Q
I7 Tumor exosomes and translational research in NPC
Pierre Busson, Claire Lhuillier, Olivier Morales, Dhafer Mrizak, Aurore Gelin, Nikiforos Kapetanakis, Nadira Delhem
I8 Host manipulations of the Epstein-Barr virus EBNA1 protein
Sheila Mansouri, Jennifer Cao, Anup Vaidya, and Lori Frappier
I9 Somatic genetic changes in EBV-associated nasopharyngeal carcinoma
Lo Kwok Wai
I10 Preliminary screening results for nasopharyngeal carcinoma with ELISA-based EBV antibodies in Southern China
Sui-Hong Chen, Jin-lin Du, Ming-Fang Ji, Qi-Hong Huang, Qing Liu, Su-Mei Cao
I11 EBV array platform to screen for EBV antibodies associated with NPC and other EBV-associated disorders
Denise L. Doolan, Anna Coghill, Jason Mulvenna, Carla Proietti, Lea Lekieffre, Jeffrey Bethony, and Allan Hildesheim
I12 The nasopharyngeal carcinoma awareness program in Indonesia
Renske Fles, Sagung Rai Indrasari, Camelia Herdini, Santi Martini, Atoillah Isfandiari, Achmad Rhomdoni, Marlinda Adham, Ika Mayangsari, Erik van Werkhoven, Maarten Wildeman, Bambang Hariwiyanto, Bambang Hermani, Widodo Ario Kentjono, Sofia Mubarika Haryana, Marjanka Schmidt, IB Tan
I13 Current advances and future direction in nasopharyngeal cancer management
Brian O’Sullivan
I14 Management of juvenile nasopharyngeal cancer
Enis Ozyar
I15 Global pattern of nasopharyngeal cancer: correlation of outcome with access to radiotherapy
Anne WM Lee
I16 The predictive/prognostic biomarker for nasopharyngeal carcinoma
Mu-Sheng Zeng
I17 Effect of HLA and KIR polymorphism on NPC risk
Xiaojiang Gao, Minzhong Tang, Pat Martin, Yi Zeng, Mary Carrington
I18 Exploring the Association between Potentially Neutralizing Antibodies against EBV Infection and Nasopharyngeal Carcinoma
Anna E Coghill, Wei Bu, Hanh Nguyen, Wan-Lun Hsu, Kelly J Yu, Pei-Jen Lou, Cheng-Ping Wang, Chien-Jen Chen, Allan Hildesheim, Jeffrey I Cohen
I19 Advances in MR imaging in NPC
Ann D King
O1 Epstein-Barr virus seromarkers and risk of nasopharyngeal carcinoma: the gene-environment interaction study on nasopharyngeal carcinoma in Taiwan
Yin-Chu Chien, Wan-Lun Hsu, Kelly J Yu, Tseng-Cheng Chen, Ching-Yuan Lin, Yung-An Tsou, Yi-Shing Leu, Li-Jen Laio, Yen-Liang Chang, Cheng-Ping Wang, Chun-Hun Hua, Ming-Shiang Wu, Chu-Hsing Kate Hsiao, Jehn-Chuan Lee, Ming-Hsui Tsai, Skye Hung-Chun Cheng, Pei-Jen Lou, Allan Hildesheim, Chien-Jen Chen
O2 Familial tendency and environmental co-factors of nasopharyngeal carcinoma: the gene-environment interaction study on nasopharyngeal carcinoma in Taiwan
Wan-Lun Hsu, Kelly J Yu, Yin-Chu Chien, Tseng-Cheng Chen, Ching-Yuan Lin, Yung-An Tsou, Yi-Shing Leu, Li-Jen Liao, Yen-Liang Chang, Tsung-Lin Yang, Chun-Hun Hua, Ming-ShiangWu, Chu-Hsing Kate Hsiao, Jehn-ChuanLee, Ming-Hsui Tsai, Skye Hung-Chun Cheng, Jenq-Yuh Ko, Allan Hildesheim, Chien-Jen Chen
O3 The genetic susceptibility and prognostic role of TERT-CLPTM1L and genes in DNA damage pathways in NPC
Josephine Mun Yee Ko, Wei Dai, Dora Kwong, Wai Tong Ng, Anne Lee, Roger Kai Cheong Ngan, Chun Chung Yau, Stewart Tung, Maria Li Lung
O4 Long term effects of NPC screening
Mingfang Ji, Wei Sheng, Mun Hon Ng, Weimin Cheng, Xia Yu, Biaohua Wu, Kuangrong Wei, Jun Zhan, Yi Xin Zeng, Su Mei Cao, Ningshao Xia, Yong Yuan
O5 Risk prediction of nasopharyngeal carcinoma by detecting host genetic and Epstein-Barr virus variation in saliva
Qian Cui, Miao Xu, Jin-Xin Bei, Yi-Xin Zeng
O6 Patterns of care study in Turkish nasopharyngeal cancer patients (NAZOTURK): A Turkish Radiation Oncology Association Head and Neck Cancer Working Group Study
B Şahin, A Dizman, M Esassolak, A Saran İkizler, HC Yıldırım, M Çaloğlu, B Atalar, F Akman, C Demiroz, BM Atasoy, E Canyilmaz, S Igdem, G Ugurluer, T Kütük, M Akmansoy, E Ozyar
O7 Long term outcome of intensity modulated radiotherapy in nasopharyngeal carcinoma in National Cancer Centre Singapore
Kiattisa Sommat, Fu Qiang Wang, Li-Lian Kwok, Terence Tan, Kam Weng Fong, Yoke Lim Soong, Shie Lee Cheah, Joseph Wee
O8 International phase II randomized study on the addition of docetaxel to the combination of cisplatin and 5-fluorouracil in the induction treatment for nasopharyngeal carcinoma in children and adolescents
M Casanova, E Özyar, C Patte, D Orbach, A Ferrari, VF Cristine, H Errihani, J Pan, L Zhang, S Liji, K Grzegorzewski, L Gore, A Varan
O9 Prognostic impact of metastatic status in patients with nasopharyngeal carcinoma
Susanna Hilda Hutajulu, Guntara Khuzairi, Camelia Herdini, Henry Kusumo, Mardiah Suci Hardianti, Kartika Widayati Taroeno-Hariadi, Ibnu Purwanto, Johan Kurnianda
O10 Development of small molecule inhibitors of latent Epstein-Barr virus infection for the treatment of nasopharyngeal carcinoma
Troy E. Messick, Kimberly Malecka, Lois Tolvinski, Samantha Soldan, Julianna Deakyne, Hui Song, Antonio van den Heuvel, Baiwei Gu, Joel Cassel, Mark McDonnell, Garry R Smith, Venkata Velvadapu, Haiyan Bian, Yan Zhang, Marianne Carlsen, Shuai Chen, Alastair Donald, Christian Lemmen, Allen B Reitz, Paul M Lieberman
O11 Therapeutic targeting of cancer stem-like cells using a Wnt modulator, ICG-001, enhances the treatment outcome of EBV-positive nasopharyngeal carcinoma
King Chi Chan, Lai Sheung Chan, Kwok Wai Lo, Timothy Tak Chun Yip, Roger Kai Cheong Ngan, Michael Kahn, Maria Li Lung, Nai Ki Mak
O12 Role of micro-RNA in NPC biology
Fei-Fei Liu
O13 Expansion of EBNA1- and LMP2-specific effector T lymphocytes from patients with nasopharyngeal carcinoma without enhancement of regulatory T cells
Wafa Khaali; Juliette Thariat; Laurence Fantin; Flavia Spirito; Meriem Khyatti; El Khalil Ben Driss; Sylvain Olivero; Janet Maryanski; Alain Doglio
O14 The experience of patients’ life after amifostine radiotherapy treatment (ART) for nasopharyngeal carcinoma (NPC)
Mengxue Xia, Yunfei Xia, Hui Chang, Rachel Shaw
O15 Analysis of mitochondrial DNA mutation in latent membrane protein-1 positive nasopharyngeal carcinoma
Pudji Rahaju
O16 Factors influencing treatment adherence of nasopharyngeal cancer and the clinical outcomes: a hospital-based study
Mardiah Suci Hardianti, Sindhu Wisesa, Kartika Widayati Taroeno-Harijadi, Ibnu Purwanto, Bambang Hariwiyanto, Wigati Dhamiyati, Johan Kurnianda
O17 Chromosomal breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells: in relation to matrix association region/scaffold attachment region
Sang-Nee Tan, Sai-Peng Sim
O18 Expression of p53 (wild type) on nasopharyngeal carcinoma stem cell that resistant to radiotherapy
Muhtarum Yusuf, Ahmad C Romdhoni, Widodo Ario K, Fedik Abdul Rantam
O19 Mathematical model of nasopharyngeal carcinoma in cellular level
Sugiyanto, Lina Aryati, Fajar Adi-Kusumo, Mardiah Suci Hardianti
O20 Differential expression of microRNA-21 on nasopharyngeal carcinoma plasma patient
SY Bintoro, R Oktriani, C. Herawati, A Surono, Sofia M. Haryana
O21 Therapeutic targeting of an oncogenic fibroblast growth factor-FGF19, which promotes proliferation and induces EMT of carcinoma cells through activating ERK and AKT signaling
L. Zhong, L. Li, B. B. Ma, A. T. Chan, Q. Tao
O22 Resist nasopharyngeal carcinoma (NPC): next generation T cells for the adoptive immunotherapy of NPC
M. Kalra, M. Ngo, S. Perna, A. Leen, N. Lapteva, C. M. Rooney, S. Gottschalk
O23 The correlation of heat shock protein 70 expressions and staging of nasopharyngeal carcinoma
Elida Mustikaningtyas, Sri Herawati, Achmad C Romdhoni
O24 Epstein-Barr virus serological profiles of nasopharyngeal carcinoma - A tribute to Werner Henle
Mingfang Ji, YaruiXu, Weimin Cheng, ShengxiangGe, Fugui Li, M. H. Ng
O25 Targeting the apoptosis pathway using combination TLR3 agonist with anti-survivin molecule (YM-155) in nasopharyngeal carcinoma
Louise SY Tan, Benjamin Wong, CM Lim
O26 The resistance mechanism of nasopharyngeal cancer stem cells to cisplatin through expression of CD44, Hsp70, p53 (wild type), Oct-4, and ß-catenin encoded-genes
Achmad C Romdhoni, Fedik A. Rantam, Widodo Ario Kentjono
P1 Prevalence of nasopharyngeal carcinoma patients at Departement of Otorhinolaringology-Head and Neck Surgery, Dr. Hasan Sadikin general hospital, Bandung, Indonesia in 2010-2014
Deasy Z Madani, Nur Akbar, Agung Dinasti Permana
P2 Case report on pediatric nasopharyngeal carcinoma at Dr. Sardjito Hospital, Yogyakarta
Camelia Herdini, Sagung Rai Indrasari, Jajah Fachiroh, Dwi Hartati, T. Baning Rahayudjati
P3 Report on loco regionally advanced nasopharyngeal cancer patients treated with induction chemotherapy followed by concurrent chemo-radiation therapy
Iswandi Darwis, Susanna Hilda Hutajulu, Bambang Hariwiyanto, Wigati Dhamiyati, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda
P4 Sex and age differences in the survival of patients with nasopharyngeal carcinoma
Sindhu Wisesa, Mardiah Suci Hardianti, Susanna Hilda Hutajulu, Kartika Widayati Taroeno-Harijadi, Ibnu Purwanto, Camelia Herdini, Wigati Dhamiyati, Johan Kurnianda
P5 Impact of delayed diagnosis and delayed therapy in the treatment outcome of patients with nasopharyngeal carcinoma
Khoirul Anwar, Susanna Hilda Hutajulu, Sagung Rai Indrasari, Sri Retna Dwidanarti, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda
P6 Anaysis of pretreatment anemia in nasopharyngeal cancer patients undergoing neoadjuvant therapy
Dominicus Wendhy Pramana, Susanna Hilda Hutajulu, Bambang Hariwiyanto, Wigati Dhamiyati, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda
P7 Results of treatment with neoadjuvant cisplatin-5FU in locally advanced nasopharyngeal carcinoma: a local experience
Diah Ari Safitri, Susanna Hilda Hutajulu, Camelia Herdini, Sri Retna Dwi Danarti, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda
P8 Geriatrics with nasopharyngeal cancer
Suryo A Taroeno, Sindhu Wisesa, Kartika Widayati Taroeno-Hariadi, Ibnu Purwanto, Bambang Hariwiyanto, Wigati Dhamiyati, Johan Kurnianda
P9 Correlation of lymphocyte to monocyte and neutrophil to lymphocyte ratio to the response of cisplatin chemoradiotheraphy in locally advance nasopharyngeal carcinoma
I. Wijaya, A. Oehadian, D. Prasetya
P10 Prediction of nasopharyngeal carcinoma risk by Epstein-Barr virus seromarkers and environmental co-factors: the gene-environment interaction study on nasopharyngeal carcinoma in Taiwan
Wan-Lun Hsu, Yin-Chu Chien, Kelly J Yu, Cheng-Ping Wang, Ching-Yuan Lin, Yung-An Tsou, Yi-Shing Leu, Li-Jen Liao, Yen-Liang Chang191,192, Jenq-Yuh Ko, Chun-Hun Hua, Ming-Shiang Wu, Chu-Hsing Kate Hsiao, Jehn-Chuan Lee, Ming-Hsui Tsai, Skye Hung-Chun Cheng, Pei-Jen Lou, Allan Hildesheim, Chien-Jen Chen
P11 Non-viral risk factors for nasopharyngeal carcinoma in West Sumatra, Indonesia
Sukri Rahman, Bestari J. Budiman, Novialdi, Rahmadona, Dewi Yuri Lestari
P12 New prototype Vidas EBV IgA quick: performance on Chinese and Moroccan populations
C. Yin, A. Foussadier, E. Blein, C. Chen, N. Bournet Ammour, M. Khiatti, S. Cao
P13 The expression of EBV-LMP1 and VEGF as predictors and plasma EBV-DNA levels as early marker of distant metastasis after therapy in nasopharyngeal cancer
Dewi Syafriyetti Soeis Marzaini
P14 Characteristics and factors influencing subjects refusal for blood samples retrieval: lesson from NPC case control study in Yogyakarta – Indonesia
Dwi Hartati, Baning Rahayujati, Camelia Herdini, Jajah Fachiroh
P15 Expression of microRNA BART-7-3p and mRNA PTEN on blood plasma of patients with nasopharyngeal carcinoma
L. Gunawan, S. Mubarika Haryana, A. Surono, C. Herawati
P16 IgA response to native early antigen (IgA-EAext) of Epstein-Barr virus (EBV) in healthy population and nasopharyngeal carcinoma (NPC) patients: the potential for diagnosis and screening tools
Michael Hartono, Jajah Fachiroh, Umi Intansari, Dewi Kartikawati Paramita
P17 IgA responses against Epstein-Barr Virus Early Antigen (EBV-EA) peptides as potential candidates of nasopharyngeal carcinoma detection marker
Akmal Akbar, Jajah Fachiroh, Dewi Kartikawati Paramita
P18 Association between smoking habit and IgA-EBV titer among healthy individuals in Yogyakarta, Indonesia
Benny Hermawan, T Baning Rahayudjati, Dewi K Paramita, Jajah Fachiroh
P19 Epstein-Barr virus IgA titer comparison of healthy non-family individuals and healthy first degree family of NPV patients
Gabriella Argy, Jajah Fachiroh, Dewi Kartikawati Paramita, Susanna Hilda Hutajulu
P20 Identification of EBV Early Antigen (EA) derived peptides for NPC diagnosis
Theodora Caroline Sihotang, Jajah Fachiroh, Umi Intansari, Dewi Kartikawati Paramita
P21 Host-pathogen study: relative expression of mRNA BRLF1 Epstein-Barr virus as a potential biomarker for tumor progressivity and polymorphisms of TCRBC and TCRGC2 host genes related to genetic susceptibility on nasopharyngeal carcinoma
Daniel Joko Wahyono, Purnomo Soeharso, Dwi Anita Suryandari, Lisnawati, Zanil Musa, Bambang Hermani
P22 In vitro efficacy of silvestrol and episilvestrol, isolated from Borneo, on nasopharyngeal carcinoma, a major cancer in Borneo
Maelinda Daker, Yeo Jiun Tzen, Norhasimah Bakar, Asma’ Saiyidatina Aishah Abdul Rahman, Munirah Ahmad, Yeo Tiong Chia, Alan Khoo Soo Beng
P23 The expression of mir-141 in patients with nasopharyngeal cancer
Widyandani Sasikirana, Tirta Wardana, Muhammad Radifar, Cita Herawati, Agus Surono, Sofia Mubarika Haryana
doi:10.1186/s12919-016-0001-5
PMCID: PMC4896251
4.  Ubiquilin-2 (UBQLN2) binds with high affinity to the C-terminal region of TDP-43 and modulates TDP-43 levels in H4 cells: characterization of inhibition by nucleic acids and 4-aminoquinolines 
Biochimica et biophysica acta  2013;1834(6):964-971.
Recently, it was reported that mutations in the ubiqutin-like protein ubiquilin-2 (UBQLN2) are associated with X-linked amyotrophic lateral sclerosis (ALS), and that both wild-type and mutant UBQLN2 can co-localize with aggregates of C-terminal fragments of TAR DNA binding protein (TDP-43). Here, we describe a high affinity interaction between UBQLN2 and TDP-43 and demonstrate that overexpression of both UBQLN2 and TDP-43 reduces levels of both exogenous and endogenous TDP-43 in human H4 cells. UBQLN2 bound with high affinity to both full length TDP-43 and a C-terminal TDP-43 fragment (261-414 aa) with KD values of 6.2 nM and 8.7 nM, respectively. Both DNA oligonucleotides and 4-aminoquinolines, which bind to TDP-43, also inhibited UBQLN2 binding to TDP-43 with similar rank order affinities compared to inhibition of oligonucleotide binding to TDP-43. Inhibitor characterization experiments demonstrated that the DNA oligonucleotides noncompetitively inhibited UBQLN2 binding to TDP-43, which is consistent with UBQLN2 binding to the C-terminal region of TDP-43. Interestingly, the 4-aminoquinolines were competitive inhibitors of UBQLN2 binding to TDP-43, suggesting that these compounds also bind to the C-terminal region of TDP-43. In support of the biochemical data, co-immunoprecipation experiments demonstrated that both TDP-43 and UBQLN2 interact in human neuroglioma H4 cells. Finally, overexpression of UBQLN2 in the presence of overexpressed full length TDP-43 or C-terminal TDP-43 (170-414) dramatically lowered levels of both full length TDP-43 and C-terminal TDP-43 fragments (CTFs). Consequently, these data suggest that UBQLN2 enhances the clearance of TDP-43 and TDP-43 CTFs and therefore may play a role in the development of TDP-43 associated neurotoxicity.
doi:10.1016/j.bbapap.2013.03.020
PMCID: PMC3960997  PMID: 23541532
TDP-43; UBQLN2; 4-aminoquinolines; protein-protein interactions
5.  Characterization of a series of 4-aminoquinolines that stimulate caspase-7 mediated cleavage of TDP-43 and inhibit its function 
Biochimie  2012;94(9):1974-1981.
Dysfunction of the heterogeneous ribonucleoprotein TAR DNA binding protein 43 (TDP-43) is associated with neurodegeneration in diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Here we examine the effects of a series of 4-aminoquinolines with affinity for TDP-43 upon caspase-7-induced cleavage of TDP-43 and TDP-43 cellular function. These compounds were mixed inhibitors of biotinylated TG6 binding to TDP-43, binding to both free and occupied TDP-43. Incubation of TDP-43 and caspase-7 in the presence of these compounds stimulated caspase-7 mediated cleavage of TDP-43. This effect was antagonized by the oligonucleotide TG12, prevented by denaturing TDP-43, and exhibited a similar relation of structure to function as for the displacement of bt-TG6 binding to TDP-43. In addition, the compounds did not affect caspase-7 enzyme activity. In human neuroglioma H4 cells, these compounds lowered levels of TDP-43 and increased TDP-43 C-terminal fragments via a caspase-dependent mechanism. Subsequent experiments demonstrated that this was due to induction of caspases 3 and 7 leading to increased PARP cleavage in H4 cells with similar rank order of the potency among the compounds tests for displacement of bt-TG6 binding. Exposure to these compounds also reduced HDAC6, ATG7, and increased LC3B, consistent with the effects of TDP-43 siRNA described by other investigators. These data suggest that such compounds may be useful biochemical probes to further understand both the normal and pathological functions of TDP-43, and its cleavage and metabolism promoted by caspases.
doi:10.1016/j.biochi.2012.05.020
PMCID: PMC3402613  PMID: 22659571
TDP-43; caspase activation; mixed inhibition
6.  Development of a Novel Nonradiometric Assay for Nucleic Acid Binding to TDP-43 Suitable for High-Throughput Screening Using AlphaScreen® Technology 
Journal of biomolecular screening  2010;15(9):1099-1106.
TAR DNA binding protein 43 (TDP-43) is a nucleic acid binding protein that is associated with the pathology of cystic fibrosis and neurodegenerative diseases such as amyotrophic lateral sclerosis and frontotemporal lobar dementia. We have developed a robust, quantitative, nonradiometric high-throughput assay measuring oligonucleotide binding to TDP-43 using AlphaScreen® technology. Biotinylated single-stranded TAR DNA (bt-TAR-32) and 6 TG repeats (bt-TG6) bound with high affinity to TDP-43, with KD values of 0.75 nM and 0.63 nM, respectively. Both oligonucleotides exhibited slow dissociation rates, with half-lives of 750 min for bt-TAR-32 and 150 min for bt-TG6. The affinities of unlabeled oligonucleotides, as determined by displacement of either bt-TAR-32 or bt-TG6, were consistent with previous reports of nucleic acid interactions with TDP-43, where increasing TG or UG repeats yield greater affinity. A diversity library of 7360 compounds was screened for inhibition of TDP-43 binding to bt-TAR-32, and a series of compounds was discovered with nascent SAR and IC50 values ranging from 100 nM to 10 μM. These compounds may prove to be useful biochemical tools to elucidate the function of TDP-43 and may lead to novel therapeutics for indications where the TDP-43 nucleic acid interaction is causal to the associated pathology.
doi:10.1177/1087057110382778
PMCID: PMC3426361  PMID: 20855563
TDP-43; AlphaScreen; TAR DNA; ALS; cystic fibrosis
7.  Design and Synthesis of Imidazopyrimidine Derivatives as Potent iNOS Dimerization Inhibitors 
A series of imidazopyrimidine derivatives with the general formula I was synthesized and identified as potent inhibitors of iNOS dimer formation, a prerequisite for proper functioning of the enzyme. Stille and Negishi coupling reactions were used as key steps to form the carbon-carbon bond connecting the imidazopyrimidine core to the central cycloalkenyl, cycloalkyl and phenyl ring templates.
doi:10.2174/1874104500903010008
PMCID: PMC2788740  PMID: 19966921

Results 1-7 (7)