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2.  Changes in Hippocampal Adenosine Efflux, ATP Levels, and Synaptic Transmission Induced by Increased Temperature 
Synapse (New York, N.Y.)  2001;41(1):58-64.
Previous studies have demonstrated that when the temperature of hippocampal brain slices is increased, there is a corresponding depression of synaptic potentials mediated by an increased activation of presynaptic adenosine A1 receptors. The present experiments demonstrate that when the temperature of hippocampal slices is raised from 32.5°C to either 38.5°C or 40.0°C there is a marked, temperature-dependent increase in the efflux of endogenous adenosine and a corresponding decrease in excitatory synaptic responses. The increase in efflux is rapidly reversible on lowering the slice temperature and the temperature-induced efflux is repeatable. Control experiments suggest that this increased efflux of adenosine is not the result of hypoxia or ischemia secondary to a temperature-induced increase in the metabolic rate of the slice. The increase in adenosine efflux was not accompanied by any significant change in the ATP levels in the brain slice, whereas a hypoxic stimulus sufficient to produce a comparable depression of excitatory transmission produced an ~75% decrease in ATP levels. These experiments indicate that changes in brain slice temperature can alter purine metabolism in such a way as to increase the adenosine concentration in the extracellular space, as well as adenosine efflux from hippocampal slices, in the absence of significant changes in ATP levels.
PMCID: PMC2213910  PMID: 11354014
adenosine; adenosine receptors; hippocampus; hypoxia; hyperthermia

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