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Applied and Environmental Microbiology (1)
Journal of Nematology (1)
Morra, Matthew J. (2)
Dick, Warren A. (1)
Meyer, Susan L. F. (1)
Orisajo, Samuel B. (1)
Zasada, Inga A. (1)
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Mustard seed meal mixtures: management of Meloidogyne incognita on pepper and potential phytotoxicity
Meyer, Susan L. F.
Zasada, Inga A.
Orisajo, Samuel B.
Journal of Nematology
Meals produced when oil is extracted from seeds in the Brassicaceae have been shown to suppress weeds and soilborne pathogens. These seed meals are commonly used individually as soil amendments; the goal of this research was to evaluate seed meal mixes of Brassica juncea (Bj) and Sinapis alba (Sa) against Meloidogyne incognita. Seed meals from Bj ‘Pacific Gold’ and Sa ‘IdaGold’ were tested alone and in combinations to determine rates and application times that would suppress M. incognita on pepper (Capsicum annuum) without phytotoxicity. Rates of soil application (% w/w) for the phytotoxicity study were: 0.5 Sa, 0.2 Bj, 0.25 Sa + 0.25 Bj, 0.375 Sa + 0.125 Bj, 0.125 Sa + 0.375 Bj, and 0, applied 0 – 5 weeks before transplant. Overall, 0.2% Bj was the least toxic meal to pepper seedlings. By comparison, 0.5% S. alba seed meal did not reduce lettuce (Lactuca sativa) seed germination at week 0, but all seed meal treatments containing B. juncea prevented or significantly reduced germination at week 0. The seed meals did not affect lettuce seed germination at weeks 1-5, but hypocotyl growth was reduced by all except 0.2% Bj at weeks 1, 4 and 5. Brassica juncea and Sa meals were tested for M. incognita suppression at 0.2, 0.15, 0.1 and 0.05%; mixtures were 0.1% Sa + 0.1% Bj, 0.15% Sa + 0.05% Bj, and 0.05% Sa + 0.15% Bj. All treatments were applied 2 weeks before transplant. The 0.2% Bj and 0.05% Sa + 0.15% Bj treatments overall had the longest shoots and highest fresh weights. Eggs per g root were lowest with 0.1 – 0.2% Bj amendments and the seed meal mixtures. The results indicate that Bj and some Bj + Sa mixtures can be applied close to transplant to suppress M. incognita populations on pepper; consequently, a seed meal mixture could be selected to provide activity against more than one pest or pathogen. For pepper, care should be taken in formulating mixtures so that Sa rates are low compared to Bj.
amendment; biofuel byproducts; Brassica; glucosinolate; management; Meloidogyne incognita; mustard seed meal; root-knot nematode; Sinapis
Mechanisms of H2S Production from Cysteine and Cystine by Microorganisms Isolated from Soil by Selective Enrichment
Dick, Warren A.
Applied and Environmental Microbiology
Hydrogen sulfide (H2S) is a major component of biogenic gaseous sulfur emissions from terrestrial environments. However, little is known concerning the pathways for H2S production from the likely substrates, cysteine and cystine. A mixed microbial culture obtained from cystine-enriched soils was used in assays (50 min, 37°C) with 0.05 M Tris-HCl (pH 8.5), 25 μmol of l-cysteine, 25 μmol of l-cystine, and 0.04 μmol of pyridoxal 5′-phosphate. Sulfide was trapped in a center well containing zinc acetate, while pyruvate was measured by derivatization with 2,4-dinitrophenylhydrazine. Sulfide and total pyruvate production were 17.6 and 17.2 nmol mg of protein-1 min-1, respectively. Dithiothreitol did not alter reaction stoichiometry or the amount of H2S and total pyruvate, whereas N-ethylmaleimide reduced both H2S and total pyruvate production equally. The amount of H2S produced was reduced by 96% when only l-cystine was included as the substrate in the assay and by 15% with the addition of propargylglycine, a specific suicide inhibitor of cystathionine γ-lyase. These data indicate that the substrate for the reaction was cysteine and the enzyme responsible for H2S and pyruvate production was cysteine desulfhydrase (EC 188.8.131.52). The enzyme had a Km of 1.32 mM and was inactivated by temperatures greater than 60°C. Because cysteine is present in soil and cysteine desulfhydrase is an inducible enzyme, the potential for H2S production by this mechanism exists in terrestrial environments. The relative importance of this mechanism compared with other processes involved in H2S production from soil is unknown.
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