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1.  Nematicides Enhance Growth and Yield of Rotylenchulus Reniformis Resistant Cotton Genotypes 
Journal of Nematology  2014;46(4):365-375.
Rotylenchulus reniformis resistant LONREN-1×FM966 breeding lines developed at Auburn University have demonstrated that the nematode resistance is accompanied by severe stunting, limited growth, and low yields. The objectives of this study were to evaluate the effects of applying nematicides to selected LONREN breeding lines on R. reniformis nematode populations, plant stunting, and yield. Four resistant breeding lines from the LONREN-1×FM966 cross, one susceptible line from the LONREN-1×FM966 cross, as well as LONREN-1, BARBREN-713, and the susceptible cultivar DP393 were evaluated with and without nematicides in the presence of R. reniformis. In the greenhouse, nematicides increased plant height across all genotypes compared with no nematicide. Rotylenchulus reniformis populations were 50% lower in the resistant lines compared with the susceptible lines at 45 days after planting (DAP). In microplot and field trials, the phenotypic stunting of all genotypes was reduced by aldicarb with increases in plant heights at 30 and 75 DAP. Increases in yields were evident across all genotypes treated with aldicarb. In all three trial environments, BARBREN-713 outperformed the LONREN-derived lines as well as ‘DP393’ in seed cotton yields, while having significantly lower R. reniformis egg densities than the susceptible genotypes.
PMCID: PMC4284089  PMID: 25580030
cotton; nematicides; reniform nematode; resistance; tolerance
2.  Our College mosaic 
Canadian Family Physician  2014;60(11):1049.
PMCID: PMC4229167  PMID: 25392445
4.  Reflecting on end-of-life care 
Canadian Family Physician  2014;60(10):953.
PMCID: PMC4196825  PMID: 25316754
6.  Family medicine hospital care 
Canadian Family Physician  2014;60(9):857.
PMCID: PMC4162705  PMID: 25217684
8.  Balancing life and work 
Canadian Family Physician  2014;60(8):761.
PMCID: PMC4131968  PMID: 25122822
10.  You are welcome here 
Canadian Family Physician  2014;60(7):681.
PMCID: PMC4096273  PMID: 25022646
12.  Looking back and moving forward 
Canadian Family Physician  2014;60(6):585.
PMCID: PMC4055334  PMID: 24925957
14.  Poetry, decisions, and societal need 
Canadian Family Physician  2014;60(5):489.
PMCID: PMC4020656  PMID: 24829013
17.  Finding the path together 
Canadian Family Physician  2014;60(4):393.
PMCID: PMC4046557  PMID: 24733334
18.  The CFPC and the SRPC 
Canadian Family Physician  2014;60(3):297.
PMCID: PMC3952769  PMID: 24627388
22.  An exciting time of year 
Canadian Family Physician  2014;60(1):93.
PMCID: PMC3994789  PMID: 24452572
24.  The Effect of Soil Texture and Irrigation on Rotylenchulus reniformis and Cotton 
Journal of Nematology  2013;45(2):99-105.
The reniform nematode, Rotylenchulus reniformis, is the most damaging nematode pathogen of cotton in Alabama. Soil texture is currently being explored as a basis for the development of economic thresholds and management zones within a field. Trials to determine the reproductive potential of R. reniformis as influenced by soil type were conducted in microplot and greenhouse settings during 2008 to 2010. Population density of R. reniformis was significantly influenced by soil texture and exhibited a general decrease with increasing median soil particle size (MSPS). As the MSPS of a soil increased from 0.04 mm in clay soil to > 0.30 mm in very fine sandy loam and sandy loam soils, R. reniformis numbers decreased. The R. reniformis population densities on all soil types were also greater with irrigation. Early season cotton development was significantly affected by increasing R. reniformis Pi, with plant shoot-weight-to-root-weight ratios increasing at low R. reniformis Pi and declining with increasing R. reniformis Pi. Plant height was increased by irrigation throughout the growing season. The results suggests that R. reniformis will reach higher population densities in soils with smaller MSPS; however, the reduction in yield or plant growth very well may be no greater than in a soil that is less preferential to the nematode.
PMCID: PMC3700743  PMID: 23833324
Gossypium hirsutum; median soil particle size (MSPS); soil moisture; soil texture; site-specific management
25.  Characterization of the Two Intra-Individual Sequence Variants in the 18S rRNA Gene in the Plant Parasitic Nematode, Rotylenchulus reniformis 
PLoS ONE  2013;8(4):e60891.
The 18S rRNA gene is fundamental to cellular and organismal protein synthesis and because of its stable persistence through generations it is also used in phylogenetic analysis among taxa. Sequence variation in this gene within a single species is rare, but it has been observed in few metazoan organisms. More frequently it has mostly been reported in the non-transcribed spacer region. Here, we have identified two sequence variants within the near full coding region of 18S rRNA gene from a single reniform nematode (RN) Rotylenchulus reniformis labeled as reniform nematode variant 1 (RN_VAR1) and variant 2 (RN_VAR2). All sequences from three of the four isolates had both RN variants in their sequences; however, isolate 13B had only RN variant 2 sequence. Specific variable base sites (96 or 5.5%) were found within the 18S rRNA gene that can clearly distinguish the two 18S rDNA variants of RN, in 11 (25.0%) and 33 (75.0%) of the 44 RN clones, for RN_VAR1 and RN_VAR2, respectively. Neighbor-joining trees show that the RN_VAR1 is very similar to the previously existing R. reniformis sequence in GenBank, while the RN_VAR2 sequence is more divergent. This is the first report of the identification of two major variants of the 18S rRNA gene in the same single RN, and documents the specific base variation between the two variants, and hypothesizes on simultaneous co-existence of these two variants for this gene.
doi:10.1371/journal.pone.0060891
PMCID: PMC3623918  PMID: 23593343

Results 1-25 (29)