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1.  Synuclein-γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF-7 cells 
Oncology Letters  2013;5(4):1347-1352.
The aim of the present study was to investigate the effects of synuclein-γ (SNCG) downregulation by RNA interference (RNAi) on the clonogenicity and invasiveness of MCF-7 breast cancer cells. This study used four pairs of SNCG-specific siRNAs which were designed and cloned into the pGPU6 plasmid for introduction into an MCF-7 cell line. The SNCG knockdown efficacies of the four siRNAs were compared using the reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry. The cells’ clonogenic and invasive phenotypes were examined with clonogenic and Boyden chamber assays. In comparison with the non-specific siRNA and empty vector controls, all four SNCG siRNAs were observed to significantly inhibit SNCG expression at the mRNA and protein levels (F=481.06, P<0.001; F=147.42, P<0.0001). SNCG suppression mediated by RNAi successfully inhibited the clonogenicity (P=0.002) and invasiveness (P<0.001) of transfected MCF-7 cells. According to the results of the present study, we concluded that SNCG suppression mediated by RNAi significantly suppressed SNCG expression at the mRNA and protein levels, suggesting that SNCG suppression mediated by an RNAi strategy may become a novel approach for treating advanced breast cancer.
PMCID: PMC3629178  PMID: 23599792
breast cancer; MCF-7 cell line; RNA interference; synuclein-γ
2.  Bromidotricarbon­yl[2-phenyl-5-(pyridin-2-yl-κN)-1,3,4-oxadiazole-κN 4]rhenium(I) dichloro­methane monosolvate 
In the title rhenium(I) complex, [ReBr(C13H9N3O)(CO)3]·CH2Cl2, the dichloro­methane solvent mol­ecule is disordered over two positions with an occupancy ratio of 0.81 (15):0.19 (15). The ReI atom is coordinated by two N atoms from a 2-phenyl-5-(pyridin-2-yl-κN)-1,3,4-oxadiazole (L) ligand, three C atoms from three carbonyl groups and a Br atom in a distorted octa­hedral geometry. The three rings in L are almost coplanar (a mean plane fitted through all non-H atoms of this ligand has an r.m.s. deviation of 0.063 Å), and the carbonyl ligands are coordinated in a fac arrangement.
PMCID: PMC3050326  PMID: 21522603
3.  2-Chloro-N-methyl-N-phenyl­acetamide 
In the title compound, C9H10ClNO, the non-H atoms, excluding the phenyl group, are almost coplanar (r.m.s. deviation of the non-H atoms = 0.1015 Å). The dihedral angle formed between this plane and the benzene ring is 87.07 (5)°. Weak inter­molecular C—H⋯O inter­actions help to stabilize the packing.
PMCID: PMC3050318  PMID: 21522779
4.  Bromidotricarbon­yl[2-(pyridin-2-yl-κN)-5-p-tolyl-1,3,4-oxadiazole-κN 3]rhenium(I) dichloro­methane monosolvate 
In the title compound, [ReBr(C14H11N3O)(CO)3]·CH2Cl2, the coordination geometry of the ReI atom is a distorted ReC3N2Br octa­hedron with the carbonyl C atoms in a fac arrangement. Within the 2-(pyridin-2-yl)-5-p-tolyl-1,3,4-oxadiazole ligand, the dihedral angles between the oxadiazole ring and the pyridine (py) and benzene (bz) rings are 1.7 (2) and 7.1 (2)°, respectively, and the dihedral angle between the py and bz rings is 5.5 (2)°. In the crystal, aromatic π–π stacking between the oxadiazole rings of adjacent mol­ecules [centroid–centroid separation = 3.465 (3) Å] is seen.
PMCID: PMC3050244  PMID: 21522604
5.  Molecular Approaches to Identify Cryptic Species and Polymorphic Species within a Complex Community of Fig Wasps 
PLoS ONE  2010;5(11):e15067.
Cryptic and polymorphic species can complicate traditional taxonomic research and both of these concerns are common in fig wasp communities. Species identification is very difficult, despite great effort and the ecological importance of fig wasps. Herein, we try to identify all chalcidoid wasp species hosted by one species of fig, using both morphological and molecular methods. We compare the efficiency of four different DNA regions and find that ITS2 is highly effective for species identification, while mitochondrial COI and Cytb regions appear less reliable, possibly due to the interference signals from either nuclear copies of mtDNA, i.e. NUMTs, or the effects of Wolbachia infections. The analyses suggest that combining multiple markers is the best choice for inferring species identifications as any one marker may be unsuitable in a given case.
PMCID: PMC2993961  PMID: 21124735
6.  (E,E)-3,3′-Dimethyl-1,1′-diphenyl-4,4′-{[3-aza­pentane-1,5-diylbis(aza­nedi­yl)]bis­(phenyl­methyl­idyne)}di-1H-pyrazol-5(4H)-one 
The asymmetric unit of the title compound, C38H37N7O2, contains one half-mol­ecule, situated on a twofold rotational axis, in which one amino group is involved in intra­molecular N—H⋯O hydrogen bond and the two phenyl rings are twisted from the plane of pyrazolone ring by 26.69 (10) and 79.64 (8)°. The crystal packing exhibits no classical inter­molecular contacts.
PMCID: PMC3011678  PMID: 21589602
7.  Ethyl 3,4-dimethyl-1H-pyrrole-2-carboxyl­ate 
The non-H atoms of the title compound, C9H13NO2, are almost coplanar (r.m.s. deviation = 0.0358 Å). Weak inter­molecular N—H⋯O hydrogen bonds link the mol­ecules into zigzag chains along the b axis with graph-set motif C(5). The chains are further linked into a three-dimensional network by C—H⋯O hydrogen bonds and C—H⋯π inter­actions.
PMCID: PMC3008020  PMID: 21588658
8.  N-(4-Chloro­phen­yl)-2-(8-quinol­yloxy)acetamide monohydrate 
In the title compound, C17H13ClN2O2·H2O, the dihedral angle between the quinoline ring system and the benzene ring is 13.0 (1)°. An intra­molecular N—H⋯O hydrogen bond may influence the mol­ecular conformation. In the crystal structure, acetamide mol­ecules are linked to water mol­ecules via inter­molecular O—H⋯ N and N—H⋯O hydrogen bonds and in turn linked into chains along [010] via O—H⋯O hydrogen bonds.
PMCID: PMC3007349  PMID: 21588294

Results 1-8 (8)