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1.  Sequence Analysis of a 101-Kilobase Plasmid Required for Agar Degradation by a Microscilla Isolate 
Applied and Environmental Microbiology  2001;67(12):5771-5779.
An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurred at a surprisingly high frequency, was associated with loss of agarase activity, supporting the sequence analysis results.
doi:10.1128/AEM.67.12.5771-5779.2001
PMCID: PMC93371  PMID: 11722934

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