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author:("hawley, D")
1.  Immune response of sows and their offspring to pseudorabies virus: serum neutralization response to vaccination and field virus challenge. 
One month prior to breeding, sows were vaccinated with an attenuated pseudorabies virus vaccine or challenged with a field strain of pseudorabies virus. A third group of sows were not vaccinated or challenged before breeding. Pigs from these sows were vaccinated at 3, 6, or 12 weeks of age and challenged with virulent virus three weeks later. One pig from each litter served as an unvaccinated, unchallenged control. Serum neutralization titers of these pigs were monitored from birth until 22 weeks of age. Titers of the sows were monitored through breeding, gestation and farrowing. The maximum prefarrowing anti-pseudorabies virus titer in the field virus challenged sows occurred four weeks following challenge. A significant decline in titers occurred at farrowing. Titers rose from one week postfarrowing and then declined. Titers in the field virus infected sows were consistently two to threefold greater than those of the vaccinated sows. The maximum prefarrowing anti-pseudorabies virus titer in the vaccinated sows occurred six weeks following vaccination. The geometric mean titer in these sow's then decreased and increased for two weeks after farrowing. The results in the pigs can be summarized as follows: Pigs from control sows had a greater serological response following field virus challenge than following vaccination with a modified live virus. Pigs from control sows responded serologically to vaccination at 3, 6 and 12 weeks of age. Pigs from control sows which were challenged at 6, 9 and 15 weeks of age had similar antibody responses. Pigs from vaccinated sows had no increase in titer following vaccination at three and six weeks of age. Titers increased when these pigs were vaccinated at 12 weeks of age. There was no significant increase in mean titers of pigs from challenged sows following vaccination at 3, 6 and 12 weeks of age. Vaccinated pigs from control and vaccinated sows had a secondary response following challenge three weeks after vaccination.(ABSTRACT TRUNCATED AT 250 WORDS)
PMCID: PMC1236035  PMID: 6326982
2.  Field evaluation of test-and-removal and vaccination as control measures for pseudorabies in Missouri swine. 
Eighteen Missouri swine herds were serologically monitored to determine the efficiency of two methods for the control of pseudorabies. A serum neutralization test-and-removal procedure was effective in ten of ten herds using this method. Vaccination procedures were less reliable. Virus still circulated in five of eight vaccinated herds and a thorough epidemiological evaluation of herd status was impossible. Titers caused by vaccination could not be distinguished from those by natural infection. Vaccination also was carried out in three seronegative herds. Antipseudorabies virus titers in these herds ranged from negative to 1:16 two to three months postvaccination. A majority of the sows in these herds responded with low 1:2 or 1:4 titers following vaccination. A direct comparison of "test and removal" farms and farms that used vaccination was not possible because of the differences between the two groups of farms.
PMCID: PMC1320309  PMID: 6293689
3.  Electrophoretic studies on alkaline phosphatases in normal and zinc intoxicated rats. 
Polyacrylamide gel electrophoresis was used to characterize the isoenzymes of serum alkaline phosphatase in the rat. The electrophoresis of serum from normal rats resulted in two bands of alkaline phosphatase activity. A prominent band in serum corresponded in electrophoretic mobility to the alkaline phosphatase from bone and intestinal tissue extracts and also a slower migrating liver isoenzyme. A less prominent, fast migrating band in serum had a mobility similar to a faster migrating liver tissue extract isoenzyme. This band only represented 1-2% of the total alkaline phosphatase present in the serum of normal rats but approximately 15% of the total alkaline phosphatase in the serum of rats fed excess levels of zinc. The study also revealed an alteration in the electrophoretic mobility of alkaline phosphatase in bone homogenate by the addition of deactivated serum to the homogenate. The addition of deactivated serum did not alter the electrophoretic mobility of the liver and intestinal alkaline phosphatases in rats.
PMCID: PMC1277696  PMID: 832193
4.  Bovine mastitis in Ontario due to Mycoplasma agalactiae subsp. bovis. 
Bovine mastitis caused by Mycoplasma agalactiae subsp. bovis was first diagnosed in 16 of 55 cows in an Ontario herd in Feburary 1972. A total of 182 of 598 (30.4%) cows from 33 of 64 (51.5%) farms in widely separated areas of the province were culturally positive. Herd incidence varied from 15 to 40% with one closed herd having an incidence of 61%. Four herds were investigated culturally and serologically by the growth inhibition test for 15 months. In the acute phase the organism was present in the milk in extremely high numbers and could still be isolated from a few cows after eight to 12 months. The sera from 89.5% of the animals with clinical mycoplasma mastitis produced a zone of surface "film" and/or colony inhibition and some cows remained positive for six to 12 months. The disease was experimentally reproduced with a pure culture of the organism isolated from the milk of a cow from one of the herds.
PMCID: PMC1277538  PMID: 1000385

Results 1-4 (4)