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3.  CTAD as a universal anticoagulant 
The feasibility of CTAD (a mixture of citrate, theophylline, adenosine and dipyridamole) as a new anticoagulant for medical laboratory use was studied prospectively. Whole blood anticoagulated with CTAD exhibited results very similar to those of blood anticoagulated with EDTA on complete blood count and automated white cell differential except for a slight decrease in platelet count and mean platelet volume. Chemistry test data for plasma obtained from CTAD whole blood were close to those obtained for matched sera. Among coagulation tests, prothrombin time, activated partial thromboplastin time and fibrinogen concentrations were close to those obtained with citrate plasma. Based on the results, CTAD was judged to be a good candidate as a new anticoagulant.
doi:10.1155/S1463924603000038
PMCID: PMC2548379  PMID: 18924886
4.  Transfer of antibody against Borrelia duttonii from mother to young in ddY mice. 
Infection and Immunity  1993;61(10):4147-4152.
The route of transfer of anti-Borrelia duttonii antibody subclasses from mother to young and their role in protection against borrelial challenge infection in ddY mice were investigated. Offspring from infected and noninfected mice were segregated and nursed by noninfected or infected mothers. Enzyme-linked immunosorbent assay analysis of antibodies of the cross-suckled offspring revealed that anti-B. duttonii immunoglobulin G1 (IgG1) is transferred exclusively in milk and that IgG2a is transferred mainly in milk but also slightly through the yolk sac route. On the other hand, IgG3 is transferred mainly through the yolk sac route but also slightly in milk, whereas IgG2b is transferred through both routes but to a lesser extent. Anti-borrelial IgM was not detected in any offspring. The protective role of transferred IgG subclasses was examined by challenge infection with B. duttonii. Offspring from noninfected mice fed by infected mothers had IgG1, IgG2a, and IgG3 at challenge and were completely protected against the challenge infection. On the other hand, offspring from infected mice fed by noninfected mothers had only IgG3, and 8 of 10 were completely protected from challenge infection whereas the other 2 contracted slight and transient spirochetemia. These findings suggested that anti-borrelial IgG3 alone has considerable protective activity and that IgG1, IgG2a, or both, either by themselves or together with IgG3, have a complete protective activity against borrelial infection.
PMCID: PMC281137  PMID: 8406804
5.  Treponema pallidum specific IgM haemagglutination test for serodiagnosis of syphilis. 
The Treponema pallidum specific IgM haemagglutination (TP-IgM-HA) test uses erythrocytes sensitised with antiserum to human IgM to separate IgM from IgG in serum. Specific antitreponemal IgM captured in this way is detected by adding a second reagent comprising erythrocytes sensitised with T pallidum antigen. Eighty two serum samples from 82 patients with untreated syphilis, 521 samples from 73 patients with treated syphilis, and 1872 samples from people who did not have syphilis were examined by the 19S(IgM)-TPHA (T pallidum haemagglutination), IgM-FTA-ABS (fluorescent treponemal antibody absorbed), TP-IgM-ELISA (enzyme linked immunosorbent assay), and TP-IgM-HA tests for the presence of 19S(IgM) antibodies specific to treponemes. The sensitivity of the TP-IgM-HA test was 97.6% and the specificity was 99.7%. We also traced IgM specific to treponemes in untreated patients with primary syphilis by four different tests. The TP-IgM-HA test results clearly reflected the effect of the treatment.
PMCID: PMC1046382  PMID: 6394097
7.  Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II. 
Nucleic Acids Research  1984;12(24):9299-9307.
The nucleotide sequence of the cloned DNA, 1,478 bp in length coding for glutathione synthetase (GSH-II) of E. coli B has been determined. Amino acid and nucleotide sequence analyses have assigned the open reading frame for GSH-II, starting with the ATG near its 5' terminus. The molecular weight calculated from the predicted amino acid sequence is 35,559 daltons, being in good agreement with that of a GSH-II subunit estimated by the SDS-PAGE method. Several signal sequences conserved in the promoter regions of E. coli were found in the non-coding regions of the gsh-II gene. They include the Shine-Dalgarno sequence, the Pribnow box and the sequence conserved in the "-35 region" with a preferable spacing from each other for an efficient transcription. Downstream from the termination codon, the inverted repeat sequences were present, followed by 6 successive T's. These structural features found in the non-coding regions have suggested to be involved in regulatory functions for the gsh-II gene expression.
PMCID: PMC320462  PMID: 6393055

Results 1-7 (7)