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1.  ‎ Association Between Neuregulin-1 Gene Variant ‎‎(rs2439272) and Schizophrenia and Its Negative ‎Symptoms in an Iranian Population 
Iranian Journal of Psychiatry  2016;11(3):147-153.
Objective: Although the etiology of schizophrenia is unknown, it has a significant genetic component. ‎A number of studies have indicated that neuregulin-1 (NRG1) gene may play a role in the ‎pathogenesis of schizophrenia. In this study, we examined whether the rs2439272 of NRG1 ‎is associated with schizophrenia and its negative symptoms in an Iranian population.‎
Method: Rs2439272 was genotyped in 469 participants including 276 unrelated patients with schizophrenia and 193 healthy controls. The association of genetic risk with negative ‎symptoms (by using panss) was examined in the total, male and female samples. COCAPHASE and ‎CLUMP22 programs were used to compare the allele and genotype frequencies, and ‎general linear regression was used to analyze the quantitative dependent variables by the ‎selected variant.‎
Results: In this study, it was revealed that the G allele of rs2439272 might be an allele with the ‎increased risk of developing schizophrenia, especially in the male participants. In addition, ‎significant differences were found between the G allele and GG genotype frequencies, and negative symptoms in the total and male participants.‎
Conclusion: Our results supported the association between rs2439272 in NRG1 gene and risk of ‎schizophrenia and its negative symptoms in an Iranian population. ‎
PMCID: PMC5139949  PMID: 27928246
Negative Symptoms; Neuregulin-1 (NRG1); Positive and Negative Syndrome Scale ‎‎ (PANSS); Schizophrenia; Single Nucleotide Polymorphism (SNP)
2.  Expression, purification, and characterization of a diabody against the most important angiogenesis cell receptor: Vascular endothelial growth factor receptor 2 
Antibodies and their derivative fragments have long been used as tools in a variety of applications, in fundamental research work, biotechnology, diagnosis, and therapy. Camels produce single heavy-chain antibodies (VHH) in addition to usual antibodies. These minimal-sized binders are very robust and bind the antigen with high affinity in a monomeric state. Vascular endothelial growth factor recepror-2 (VEGFR2) is an important tumor-associated receptor that blockade of its signaling can lead to the inhibition of neovascularization and tumor metastasis. Here, we describe the construction, expression, and purification VEGFR2-specific Diabody. Two variable fragments of a same camel anti-VEGFR2 antibody were linked together by the upper hinge segment of antibody to make a diabody. We showed the ability of diabody to recognition of VEGFR2 on the cell surface by FACS. Diabodies can be produced in the low-cost prokaryotic expression system, so they are suitable molecules for diagnostic and therapeutic issues.
PMCID: PMC3544076  PMID: 23326765
Diabody; Nanobody; vascular endothelial growth factor recepror-2
3.  Thyroid Peroxidase Gene Mutation in Patients with Congenital Hypothyroidism in Isfahan, Iran 
Background. Thyroid peroxidase gene (TPO) mutations are one of the most common causes of thyroid dyshormonogenesis in patients with congenital hypothyroidism (CH). In this study, the prevalence of TPO gene mutations in patients with thyroid dyshormonogenesis in Isfahan was investigated. Methods. In this cross-sectional study, genomic DNA of 41 patients with permanent CH due to thyroid dyshormonogenesis was extracted using the salting out method. The 17 exonic regions of the TPO gene were amplified. SSCP technique was performed for scanning of the exonic regions of the TPO gene, except exon 8. DNA sequencing was performed for those with different migration patterns in SSCP by chain termination method. Exon 8 was sequenced directly in all patients. In 4 patients, all fragments were also sequenced. Results. One missense mutation c.2669G > A (NM_000547.5) at exon 15 (14th coding exon) in one patient in homozygous form and seven different single nucleotide polymorphisms (SNPs) in exons 1, 7, 8, 11, and 15 of TPO gene. Conclusion. The TPO gene mutations among CH patients with dyshormonogenesis in Isfahan were less frequent in comparison with other similar studies. It may be due to the presence of other unknown gene mutations which could not be detected by SSCP and sequencing methods.
PMCID: PMC3419406  PMID: 22919382

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