Enter Your Search:
Results 1-2 (2)
Go to page number:
Select a Filter Below
Journal of Medical Case Reports (1)
Journal of neuroendocrinology (1)
Amin, Jenny (1)
Gupta, Ravi K (1)
Hariri, Omid R (1)
Hariri, Omid R. (1)
Kuo, John (1)
Lawandy, Shokry (1)
Micevych, Paul (1)
Minasian, Tanya (1)
Miulli, Dan E (1)
Siddiqi, Javed (1)
Year of Publication
Supratentorial primitive neuroectodermal tumor in an adult: a case report and review of the literature
Miulli, Dan E
Gupta, Ravi K
Journal of Medical Case Reports
Supratentorial primitive neuroectodermal tumors predominantly occur in children, and are rare in the adult population. Less than 100 cases of supratentorial primitive neuroectodermal tumor have been reported in adults internationally. Our case study reports this rare incident.
A 22-year-old Hispanic man presented with headaches, blurry vision, diplopia, intermittent vomiting, and grossly decreased vision. A magnetic resonance image showed a left posterior parietal heterogeneously enhancing mass measuring 4.2cm × 7.2cm × 7.0cm. After craniotomy for resection and decompression, the mass was histologically revealed to be a supratentorial primitive neuroectodermal tumor. Standardized immunohistochemical studies for this mass were carried out.
We have concluded that immunohistochemical and genetic workup should be included in the standardized pathological workup for primitive neuroectodermal tumors in order to provide more prognostic information. Based on our current literature review, we propose an immunohistochemical panel.
An Interaction of Oxytocin Receptors with Metabotropic Glutamate Receptors in Hypothalamic Astrocytes
Journal of neuroendocrinology
Hypothalamic astrocytes play a critical role in the regulation and support of many different neuroendocrine events, and are affected by estradiol. Both nuclear and membrane estrogen receptors (ERs) are expressed in astrocytes. Upon estradiol activation, membrane-associated ER (mER) signals through the type 1a metabotropic glutamate receptor (mGluR1a) to induce an increase of free cytoplasmic calcium concentration ([Ca2+]i). Since the expression of oxytocin receptors (OTRs) is modulated by estradiol, we tested if estradiol also influences oxytocin signaling. Oxytocin at 1 nM, 10 nM, and 100 nM induced a [Ca2+]i flux measured as a change in relative fluorescence (ΔF Ca2+ = 330 ± 17 relative fluorescent units (RFU), ΔF Ca2+ = 331 ± 22 RFU, and ΔF Ca2+ = 347 ± 13 RFU, respectively) in primary cultures of female post-pubertal hypothalamic astrocytes. Interestingly, OTRs interacted with mGluRs. The mGluR1a antagonist, LY 367385 (20 nM), blocked the oxytocin (1 nM)-induced [Ca2+]i flux (ΔF Ca2+ = 344 ± 19 vs. 127 ± 11 RFU, P < 0.001). Conversely, the mGluR1a receptor agonist, (RS)-3,5-dihydroxyphenyl-glycine (DHPG, 100 nM), increased the oxytocin (1 nM)-induced [Ca2+]i response (ΔF Ca2+ = 670 ± 31 RFU) compared with either compound alone (P < 0.001). Since both oxytocin and estradiol rapidly signal through the mGluR1a, we treated hypothalamic astrocytes sequentially with oxytocin and estradiol to determine whether stimulation with one hormone affected the subsequent [Ca2+]i response to the second hormone. Estradiol treatment did not change the subsequent [Ca2+]i flux to oxytocin (P > 0.05) and previous oxytocin exposure did not affect the [Ca2+]i response to estradiol (P > 0.05). Furthermore, simultaneous estradiol and oxytocin stimulation failed to yield a synergistic [Ca2+]i response. These results suggest that the OTR signals through the mGluR1a to release Ca2+ from intracellular stores and rapid, non-genomic estradiol stimulation does not influence OTR signaling in astrocytes.
OTR; mGluR1a; [Ca2+]i; rapid cell signaling; mER
Results 1-2 (2)
Go to page number:
Remove citation from clipboard
Add citation to clipboard
This will clear all selections from your clipboard. Do you wish proceed?
Clipboard is full! Please remove an item and try again.
PubMed Central Canada is a service of the
Canadian Institutes of Health Research
(CIHR) working in partnership with the National Research Council's
Canada Institute for Scientific and Technical Information
in cooperation with the
National Center for Biotechnology Information
U.S. National Library of Medicine
(NCBI/NLM). It includes content provided to the
PubMed Central International archive
by participating publishers.