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Journal of Biomedicine and Biotechnology (1)
PLoS ONE (1)
Maranhão, Andrea Q. (2)
Almeida, Sandro R. (1)
Brigido, Marcelo M. (1)
Brígido, Marcelo M. (1)
Dantas-Barbosa, Carmela (1)
Faria, Fabrícia P. (1)
Ferreira, Karen S. (1)
Garcia, Maria C. C. (1)
Lopes, José D. (1)
Nielsen, Kirsten (1)
Santos, Suelen S. (1)
Year of Publication
Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis
Ferreira, Karen S.
Garcia, Maria C. C.
Brígido, Marcelo M.
Santos, Suelen S.
Lopes, José D.
Almeida, Sandro R.
Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naïve T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model.
Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library
Faria, Fabrícia P.
Brigido, Marcelo M.
Journal of Biomedicine and Biotechnology
Osteosarcoma, a highly malignant disease, is the most common primary bone tumor and is frequently found in children and adolescents. In order to isolate antibodies against osteosarcoma antigens, a combinatorial osteosarcoma Fab library displayed on the surface of phages was used. After three rounds of selection on the surface of tumor cells, several osteosarcoma-reactive Fabs were detected. From these Fabs, five were better characterized, and despite having differences in their VH (heavy chain variable domain) and Vκ (kappa chain variable domain) regions, they all bound to a protein with the same molecular mass. Further analysis by cell ELISA and immunocytochemistry suggested that the Fabs recognize a membrane-associated tumor antigen expressed in higher amounts in neoplasic cells than in normal tissue. These results suggest that the human Fabs selected in this work are a valuable tool for the study of this neoplasia.
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