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1.  Subunit Approach to Evaluation of the Immune Protective Potential of Leptospiral Antigens ▿ 
Clinical and Vaccine Immunology : CVI  2011;18(12):2026-2030.
Leptospirosis is the most widespread zoonosis in the world. Current vaccines are based on whole-cell preparations that cause severe side effects and do not induce satisfactory immunity. In light of the leptospiral genome sequences recently made available, several studies aimed at identification of protective recombinant immunogens have been performed; however, few such immunogens have been identified. The aim of this study was to evaluate 27 recombinant antigens to determine their potential to induce an immune response protective against leptospirosis in the hamster model. Experiments were conducted with groups of female hamsters immunized with individual antigen preparations. Hamsters were then challenged with a lethal dose of Leptospira interrogans. Thirteen antigens induced protective immune responses; however, only recombinant proteins LIC10325 and LIC13059 induced significant protection against mortality. These results have important implications for the development of an efficacious recombinant subunit vaccine against leptospirosis.
doi:10.1128/CVI.05297-11
PMCID: PMC3232701  PMID: 22030369
2.  In vitro antimicrobial resistance of staphylococci isolated from canine urinary tract infection 
The Canadian Veterinary Journal  2010;51(7):738-742.
This study determined the diversity of species and antimicrobial resistance of staphylococci isolated from dogs with a presumptive diagnosis of urinary tract infection (UTI). Urine samples from 348 dogs with clinical signs of UTI, according to clinical examination and urinalysis, were processed for isolation of Staphylococcus. Colonies in pure culture were identified by biochemical reactions and tested for susceptibility to 15 antimicrobials. Seventy isolates of staphylococci were obtained (20.1%). Staphylococcus pseudintermedius was the most frequent species (32.8%), followed by S. epidermidis (18.6%), S. simulans (15.7%), S. schleiferi schleiferi (11.4%), S. aureus (11.4%), S. schleiferi coagulans (7.2%) and S. saprophyticus (2.9%). All the isolates were resistant to at least 1 drug and 77.1% were multiresistant. The study reports the alarming antimicrobial resistance of members of the Staphylococcus genus isolated from canine UTI and highlights the importance of coagulase-negative staphylococci in its etiology.
PMCID: PMC2885114  PMID: 20885826
3.  Potential Application Of New Diagnostic Methods For Controlling Bovine Tuberculosis In Brazil 
Brazilian Journal of Microbiology  2010;41(3):531-541.
Bovine tuberculosis, a chronic infection in cattle caused by Mycobacterium bovis, remains an economic and public health problem for several countries. Due to its economic impact on international trade, contagious nature, and implications for human health, global programs to eradicate the disease were implemented worldwide. Those programs are based on slaughtering PPD-reactive animals. Despite the National Programs in Brazil, complete eradication has not been achieved, and the disease remains, albeit at a lower prevalence.
The central purpose of this review is to address diagnostic tests for tuberculosis. Considering the course of the infection in cattle, at least two tests, ideally complementary to one another, may be necessary for an adequate diagnosis: the first based on the cellular response, and the second capable of identifying anergic animals by detection of specific anti-M.bovis antibodies.
doi:10.1590/S1517-83822010005000002
PMCID: PMC3768653  PMID: 24031527
Bovine Tuberculosis; Mycobacterium bovis; Diagnosis
4.  Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR 
Brazilian Journal of Microbiology  2010;41(2):386-390.
Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
doi:10.1590/S1517-838220100002000020
PMCID: PMC3768681  PMID: 24031509
Mycobacterium bovis; multiplex-PCR; nasal swab; bovine tuberculosis
5.  Identification of Mycobacterium bovis Isolates by a multiplex PCR 
Brazilian Journal of Microbiology  2009;40(2):231-233.
Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.
doi:10.1590/S1517-83822009000200004
PMCID: PMC3769727  PMID: 24031349
Mycobacterium bovis; multiplex-PCR; bovine tuberculosis
6.  An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum 
Brazilian Journal of Microbiology  2008;39(3):511-513.
ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
doi:10.1590/S1517-838220080003000019
PMCID: PMC3768432  PMID: 24031256
Mycobacteria; ELISA; M. fortuitum; M. phlei

Results 1-6 (6)