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1.  New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA 
Nucleic Acids Research  2010;38(19):6533-6543.
Two archaeal tRNA methyltransferases belonging to the SPOUT superfamily and displaying unexpected activities are identified. These enzymes are orthologous to the yeast Trm10p methyltransferase, which catalyses the formation of 1-methylguanosine at position 9 of tRNA. In contrast, the Trm10p orthologue from the crenarchaeon Sulfolobus acidocaldarius forms 1-methyladenosine at the same position. Even more surprisingly, the Trm10p orthologue from the euryarchaeon Thermococcus kodakaraensis methylates the N1-atom of either adenosine or guanosine at position 9 in different tRNAs. This is to our knowledge the first example of a tRNA methyltransferase with a broadened nucleoside recognition capability. The evolution of tRNA methyltransferases methylating the N1 atom of a purine residue is discussed.
doi:10.1093/nar/gkq451
PMCID: PMC2965216  PMID: 20525789
2.  Monoclinic form of isopentenyl diphosphate isomerase: a case of polymorphism in biomolecular crystals 
Type 1 isopentenyl diphosphate isomerase (IDI-1) in a new crystal form.
Type 1 isopentenyl diphosphate isomerase (IDI-1) has been crystallized in a new crystal form. After data collection from small thin needle-shaped crystals, a new monoclinic form of the studied protein was identified. In this article, the three crystal forms of IDI-1 (orthorhombic, monoclinic and trigonal) are compared.
doi:10.1107/S174430910800568X
PMCID: PMC2374249  PMID: 18391416
IPP isomerase; polymorphism
3.  The yggH Gene of Escherichia coli Encodes a tRNA (m7G46) Methyltransferase 
Journal of Bacteriology  2003;185(10):3238-3243.
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-l-methionine-dependent formation of N7-methylguanosine at position 46 (m7G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m7G46) methyltransferase activity.
doi:10.1128/JB.185.10.3238-3243.2003
PMCID: PMC154064  PMID: 12730187

Results 1-3 (3)